LOCUS       sequence-190434-       10525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of RNF6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: RNF6_pLX307
FEATURES             Location/Qualifiers
     source          1..10525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3376..3400)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3408..3449
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3560..4148
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3613..3633)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4031..4042)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4220..4453
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4531..4652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4568..4587)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4622..4641
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4692..4827
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4847..4866)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4848..4866)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4876..4893)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4876..4892)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4885..4907)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5034..5489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5121..5140)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5331..5352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5515..5619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5620..5688,5689..6480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5838..5857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6651..7239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7140..7159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7393..7410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7527..7548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7563..7568,7569..7586,7587..7593)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7601..7617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7606..7628
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7625..7641
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7625..7641
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7660..7680
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7662..7680
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7708..7934
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7935..8115
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8162..8287
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8780..9013
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9198..9242
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9473..9683
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9495..9691
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9542..9677
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9723..10325
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9723..9742)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10219..10239
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10368..10485
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgaatcagtc tagatcgaga tcagatggtg gcagtgaaga aaccttacct caagaccata
     1381 atcatcatga aaatgagaga agatggcagc aagagcgtct ccacagagaa gaggcctatt
     1441 atcagtttat taatgaactc aatgatgaag attatcggct tatgagagac cataatcttt
     1501 taggcacccc tggagaaata acatcagaag aactgcaaca gcggttagat ggcgtcaagg
     1561 aacaactagc atctcagcct gacttgagag atggaacgaa ttacagagac tcagaagtcc
     1621 ctagagaaag ttcacatgaa gattctcttc tagaatggtt gaacaccttt cggcgcacag
     1681 gaaatgcaac tcgaagtgga caaaatggga accaaacttg gagagctgtg agtcgaacaa
     1741 acccgaacaa tggagagttt cggtttagtt tggaaatcca cgtaaatcat gaaaatagag
     1801 gatttgaaat tcatggagaa gattatacag acattccact ttcagatagt aacagagatc
     1861 atactgcaaa taggcaacaa aggtcaacta gtcctgtggc taggcgaaca agaagccaaa
     1921 cctcagtgaa tttcaatggt agtagttcca acattccaag gactaggctt gcttcaaggg
     1981 ggcaaaatcc agctgaagga tctttctcaa cattgggaag gttaagaaat ggaattgggg
     2041 gagcagctgg cattcctcga gctaacgctt cacgcactaa tttcagtagt cacacaaacc
     2101 aatcaggtgg tagtgaactc aggcaaaggg aggggcaacg gtttggagca gcacatgttt
     2161 gggaaaatgg ggctagaagt aatgttacag tgaggaatac aaaccaaaga ttagagccaa
     2221 taagattacg atctacttcc aatagtcgaa gccgttcacc aattcagaga cagagtggca
     2281 ctgtttatca taattcccaa agggaaagta gaccagtaca gcaaaccact agaagatctg
     2341 ttaggaggag aggtagaact cgagtctttt tagagcaaga tagagaacga gaacgcagag
     2401 gtactgcata taccccattc tctaattcaa ggcttgtgtc aagaataaca gtagaagaag
     2461 gagaagaatc cagcagatcc tcaactgctg tacgacgaca tccaacaatc acactggacc
     2521 ttcaagtgag aaggatccgt cctggagaaa atagagatcg ggatagtatt gcaaatagaa
     2581 ctcgatccag agtagggcta gcagaaaata cagtcactat tgaaagcaat agtgggggct
     2641 ttcgccgaac catttctcgt ttagagcggt caggtattcg aacctatgtt agtaccataa
     2701 cagttcctct tcgtaggatt tctgagaatg agcttgttga gccatcatca gtggctcttc
     2761 ggtcaatttt aaggcagatc atgactgggt ttggagaact gagttctcta atggaggccg
     2821 attctgagtc agaacttcaa agaaatggcc agcatttacc agacatgcac tcagaactga
     2881 gtaacttagg tacagataac aacaggagcc agcacaggga aggttcctct caagacaggc
     2941 aggcccaagg agacagcact gaaatgcatg gtgaaaacga gaccacccag cctcatactc
     3001 gaaacagtga cagtaggggt ggcaggcagt tgcgaaatcc aaacaattta gttgaaactg
     3061 gaacactacc cattcttcgc cttgctcact tttttttact aaatgaaagt gatgatgatg
     3121 atcgaatacg tggtttaacc aaagagcaga ttgacaatct ttccaccagg cactatgagc
     3181 ataacagtat tgatagtgaa ctaggtaaaa tctgtagtgt ttgtattagt gactatgtaa
     3241 ctggaaacaa gctcaggcaa ttaccttgca tgcatgaatt tcacattcat tgtattgacc
     3301 gatggctctc agagaattgc acttgtccga tctgtcggca gcctgtttta gggtctaaca
     3361 tagcaaacaa tgggtaccca actttcttgt acaaagtggt tgatatcggt aagcctatcc
     3421 ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa cttgaaagta
     3481 tttcgatttc ttggctttat atatcttgtg gaaaggacga ggatttgaac taagagagcc
     3541 attcacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3601 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3661 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3721 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3781 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3841 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3901 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3961 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4021 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4081 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4141 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4201 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     4261 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4321 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4381 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4441 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     4501 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     4561 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4621 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     4681 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4741 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4801 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4861 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4921 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4981 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     5041 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5101 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5161 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5221 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5281 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5341 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5401 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5461 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     5521 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5581 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5641 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5701 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5761 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5821 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5881 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5941 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6001 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6061 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6121 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6181 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6241 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6301 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6361 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6421 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6481 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6781 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7261 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7321 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7381 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7441 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7501 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7561 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7621 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     7681 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7741 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7801 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7861 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7921 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7981 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8041 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8101 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     8161 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     8221 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     8281 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     8341 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     8401 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     8461 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8521 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8581 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     8641 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     8701 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8761 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8821 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8881 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8941 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     9001 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     9061 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     9121 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     9181 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     9241 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     9301 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     9361 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc
     9421 attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9481 aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9541 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9601 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9661 aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta
     9721 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     9781 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     9841 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     9901 acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg
     9961 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
    10021 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
    10081 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
    10141 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
    10201 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
    10261 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca
    10321 gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
    10381 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
    10441 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
    10501 gacagcagag atccactttg gcgcc
//
