LOCUS       sequence-190440-        9610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of NPRL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9610)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: NPRL2_pLX307
FEATURES             Location/Qualifiers
     source          1..9610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2461..2485)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2493..2534
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2645..3233
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2698..2718)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3116..3127)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3305..3538
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3616..3737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3653..3672)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3707..3726
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3777..3912
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3932..3951)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3933..3951)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3961..3978)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3961..3977)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3970..3992)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4119..4574
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4206..4225)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4416..4437
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4600..4704
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4705..4773,4774..5565)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4923..4942)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5736..6324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6225..6244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6478..6495
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6612..6633
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6648..6653,6654..6671,6672..6678)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6686..6702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6691..6713
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6710..6726
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6710..6726
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6745..6765
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6747..6765
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6793..7019
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7020..7200
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7247..7372
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7865..8098
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8283..8327
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8558..8768
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8580..8776
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8627..8762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8808..9410
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8808..8827)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9304..9324
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9453..9570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgggcagcgg ctgccgcatc gaatgcatat tcttcagcga gttccacctc acgctgggac
     1381 ccaagatcac ctatcaggtc cctgaagact tcatctcccg agagctgttt gacacagtcc
     1441 aagtgtacat catcaccaag ccagagctgc agaacaagct tatcactgtc acagctatgg
     1501 aaaagaagct gatcggctgt cctgtgtgca tcgaacacaa gaagtacagc cgcaatgctc
     1561 tcctcttcaa cctgggcttc gtgtgtgatg cccaggccaa gacctgcgcc ctcgagccca
     1621 ttgttaaaaa gctggctggc tatctgacca cactagagct agagagcagc ttcgtgtcca
     1681 tggaggagag caagcagaag ttggtgccca tcatgaccat cttgctggag gagctaaatg
     1741 cctcaggccg gtgcactctg cccattgatg agtccaacac catccacttg aaggtgattg
     1801 agcagcggcc agaccctccg gtggcccagg agtatgatgt acctgtcttt accaaagaca
     1861 aggaggattt cttcaactca cagtgggacc tcactacaca acaaatcctg ccctacattg
     1921 atgggttccg ccacatccag aagatttcag cagaggcaga tgtggagctc aacctggtgc
     1981 gcattgctat ccagaacctg ctgtactacg gcgttgtgac actggtgtcc atcctccagt
     2041 actccaatgt atactgccca acgcccaagg tccaggacct ggtagatgac aagtccctgc
     2101 aagaggcatg tctatcctac gtgaccaagc aagggcacaa gagggccagt ctccgggatg
     2161 tgttccagct atactgcagc ctgagccctg gcactaccgt gcgagacctc attggccgcc
     2221 acccccagca gctgcagcat gttgatgaac ggaagctgat ccagttcggg cttatgaaga
     2281 acctcatcag gcgactacag aagtatcctg tgcgggtgac tcgggaagag cagagccacc
     2341 ctgcccggct ttatacaggc tgccacagct atgacgagat ctgctgcaag acaggcatga
     2401 gctaccatga gctggatgag cggcttgaaa atgaccccaa catcatcatc tgctggaagt
     2461 gcccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac cctctcctcg
     2521 gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc
     2581 tttatatatc ttgtggaaag gacgactttc actaggtata gctatctgaa cgcgttaagt
     2641 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2701 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2761 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2821 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2881 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2941 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3001 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3061 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3121 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3181 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     3241 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     3301 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     3361 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     3421 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     3481 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     3541 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     3601 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3661 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3721 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     3781 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3841 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3901 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     3961 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     4021 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4081 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     4141 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4201 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4261 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4321 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4381 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4441 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4501 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4561 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4621 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4681 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4741 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4801 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4861 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4921 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4981 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5041 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5101 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5161 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5221 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5281 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5341 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5401 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5461 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5521 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5581 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5641 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5701 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5761 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5821 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5881 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5941 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6001 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6061 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6121 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6181 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6241 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6301 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6361 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6421 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6481 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6541 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6601 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6661 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     6721 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     6781 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     6841 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     6901 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     6961 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     7021 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7081 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7141 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7201 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     7261 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     7321 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     7381 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     7441 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     7501 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     7561 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     7621 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     7681 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     7741 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     7801 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     7861 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     7921 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     7981 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     8041 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     8101 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     8161 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     8221 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     8281 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     8341 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     8401 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     8461 cattatcgtt tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta
     8521 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8581 gcgtctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     8641 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8701 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8761 cctaggcttt tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca
     8821 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     8881 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     8941 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     9001 gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg
     9061 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     9121 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     9181 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     9241 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     9301 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     9361 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag
     9421 atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     9481 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     9541 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     9601 ctttggcgcc
//
