LOCUS       sequence-190447-        9256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PRKCDPB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: PRKCDPB_pLX307
FEATURES             Location/Qualifiers
     source          1..9256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2107..2131)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2139..2180
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2291..2879
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2344..2364)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2762..2773)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2951..3184
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3262..3383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3299..3318)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3353..3372
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3423..3558
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3578..3597)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3579..3597)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3607..3624)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3607..3623)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3616..3638)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3765..4220
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3852..3871)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4062..4083
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4246..4350
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4351..4419,4420..5211)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4569..4588)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5382..5970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5871..5890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6124..6141
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6258..6279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6294..6299,6300..6317,6318..6324)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6332..6348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6337..6359
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6356..6372
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6356..6372
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6391..6411
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6393..6411
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6439..6665
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6666..6846
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6893..7018
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7511..7744
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7929..7973
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8204..8414
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8226..8422
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8273..8408
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8454..9056
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8454..8473)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8950..8970
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9099..9216
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 ccatgaggga gagtgcgttg gagccggggc ctgtgcccga ggcgccggcg gggggtcccg
     1381 tgcacgccgt gacggtggtg accctgctgg agaagctggc ctccatgctg gagactctgc
     1441 gggagcggca gggaggcctg gctcgaaggc agggaggcct ggcagggtcc gtgcgccgca
     1501 tccagagcgg cctgggcgct ctgagtcgca gccacgacac caccagcaac accttggcgc
     1561 agctgctggc caaggcggag cgcgtgagct cgcacgccaa cgccgcccaa gagcgcgcgg
     1621 tgcgccgcgc agcccaggtg cagcggctgg aggccaacca cgggctgctg gtggcgcgcg
     1681 ggaagctcca cgttctgctc ttcaaggagg agggtgaagt cccagccagc gctttccaga
     1741 aggcaccaga gcccttgggc ccggcggacc agtccgagct gggcccagag cagctggagg
     1801 ccgaagttgg agagagctcg gacgaggagc cggtggagtc cagggcccag cggctgcggc
     1861 gcaccggatt gcagaaggta cagagcctcc gaagggccct ttcgggccgg aaaggccctg
     1921 cagcgccacc gcccaccccg gtcaagccgc ctcgccttgg gcctggccgg agcgctgaag
     1981 cccagccgga agcccagcct gcgctggagc ccacgctgga gccagagcct ccgcaggaca
     2041 ccgaggaaga tcccgggaga cctggggctg ccgaagaagc tctgctccaa atggagagtg
     2101 tagccttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc
     2161 tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt atttcgattt
     2221 cttggcttta tatatcttgt ggaaaggacg atactaaaac ccttcctcgg aaccgacgcg
     2281 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     2341 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     2401 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     2461 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     2521 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     2581 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     2641 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     2701 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     2761 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     2821 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     2881 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     2941 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     3001 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     3061 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     3121 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     3181 agcagtacgt atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     3241 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     3301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3361 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     3421 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3481 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3541 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     3601 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     3661 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     3721 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     3781 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3841 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3901 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3961 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4021 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4081 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4141 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4201 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4261 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4321 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4381 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4441 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4501 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4561 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4621 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4681 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4741 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4801 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4861 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4921 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4981 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5041 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5101 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5161 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5221 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5281 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5341 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5401 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5461 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5521 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5581 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5641 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5701 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5761 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5821 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5881 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5941 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6001 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6061 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6121 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6181 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6241 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6301 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6361 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     6421 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     6481 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     6541 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     6601 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     6661 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6721 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6781 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6841 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     6901 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     6961 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     7021 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     7081 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     7141 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     7201 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     7261 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     7321 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     7381 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     7441 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     7501 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     7561 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     7621 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     7681 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     7741 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     7801 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     7861 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     7921 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     7981 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     8041 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     8101 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct
     8161 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8221 aagcatgcgt ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     8281 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8341 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8401 tggaggccta ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg
     8461 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     8521 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     8581 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     8641 aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg
     8701 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     8761 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     8821 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     8881 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     8941 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     9001 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa
     9061 ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     9121 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     9181 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     9241 gatccacttt ggcgcc
//
