LOCUS       sequence-190449-       10501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of LIMD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: LIMD1_pLX307
FEATURES             Location/Qualifiers
     source          1..10501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3352..3376)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3384..3425
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3536..4124
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3589..3609)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4007..4018)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4196..4429
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4507..4628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4544..4563)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4598..4617
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4668..4803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4823..4842)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4824..4842)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4852..4869)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4852..4868)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4861..4883)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5010..5465
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5097..5116)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5307..5328
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5491..5595
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5596..5664,5665..6456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5814..5833)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6627..7215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7116..7135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7369..7386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7503..7524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7539..7544,7545..7562,7563..7569)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7577..7593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7582..7604
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7601..7617
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7601..7617
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7636..7656
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7638..7656
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7684..7910
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7911..8091
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8138..8263
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8756..8989
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9174..9218
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9449..9659
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9471..9667
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9518..9653
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9699..10301
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9699..9718)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10195..10215
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10344..10461
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggataagta tgacgacctg ggcctggagg ccagtaaatt catcgaggac ctgaacatgt
     1381 atgaggcctc taaggatggg ctcttccgag tggacaaggg tgcaggcaac aaccccgagt
     1441 ttgaggaaac tcgcagggtg ttcgccacca agatggccaa aatccacctc cagcagcagc
     1501 agcagcagct cctgcaggag gagactctgc ccagggggag tagaggccct gtcaatggag
     1561 ggggccgcct gggcccacag gcccgttggg aagttgtggg cagcaagctg actgtggatg
     1621 gtgctgccaa gcctcctctt gctgcctcga caggggcacc tggggcagtc accaccctcg
     1681 ctgctgggca gcccccgtac ccaccgcagg agcagagatc caggccatac ctgcatggca
     1741 cgaggcatgg cagccaggac tgtggttcca gggagagcct ggcgacttct gagatgtctg
     1801 ctttccacca gccaggcccc tgtgaggatc cttcctgcct cactcatgga gactattatg
     1861 acaacctctc cttggcaagc ccaaagtggg gtgacaaacc aggagtgtcc cccagcatcg
     1921 gcctgagtgt agggagtggg tggcctagct ccccggggag tgacccacca ctgcccaaac
     1981 cctgcgggga ccatccccta aatcaccgac agctctccct gagctccagc aggtcttctg
     2041 agggtagcct cggtggtcag aatagtggca ttggtggccg cagcagcgag aagccaacag
     2101 gcctttggtc cactgcctcc tcccagcggg tgagccctgg cctgccttcc ccaaacttgg
     2161 agaacggagc accagctgtg gggcctgttc agcccaggac tccttctgtg tcagcaccct
     2221 tggccctgag ctgccccagg caaggaggtc ttccaagatc aaactcgggg ctggggggtg
     2281 aggtttcagg tgtgatgtcc aaacccaatg tggaccccca accctggttc caggatgggc
     2341 ccaaatctta cctttccagt tctgccccgt catcctcgcc agctggcctg gacggttcac
     2401 agcagggtgc ggtccctggg ctggggccga agcctggctg cacagacctt ggcactggtc
     2461 ccaagctcag ccccaccagt cttgtccatc cagtgatgtc caccctgcct gagttatctt
     2521 gtaaagaggg tcccctgggc tggtcttctg atggtagcct gggatctgtg ctcctggaca
     2581 gccccagctc ccctagggta aggctgccct gccagcccct cgtcccaggt cctgagctga
     2641 gaccctctgc tgctgagttg aaattagaag ccctcaccca acgtctggag cgagagatgg
     2701 atgctcaccc gaaggctgat tactttggag cctgtgtgaa atgcagcaaa ggggtgtttg
     2761 gggctggcca ggcctgtcag gccatgggga acctctacca tgacacatgc ttcacctgtg
     2821 cagcttgcag ccggaagctg agaggaaaag ccttttattt tgtcaacggc aaagtgtttt
     2881 gtgaagaaga cttcctgtac tctggtttcc agcagtcggc tgacaggtgt tttctttgtg
     2941 gacatctgat catggacatg atcctgcaag ccctggggaa gtcctaccac cccggctgtt
     3001 tccgctgtgt catctgtaat gagtgtttgg atggggtgcc cttcaccgtg gactcagaga
     3061 acaagatcta ctgtgtccga gattaccaca aggtgctggc ccccaagtgt gcagcctgtg
     3121 ggcttcccat ccttccacct gagggctcag atgagaccat ccgtgtcgtg tccatggaca
     3181 gagactacca cgtggagtgt taccactgcg aggactgtgg tctggagctc aatgatgaag
     3241 atggccaccg ctgttatccg ctggaggacc acctgttctg tcactcctgc cacgtgaaga
     3301 ggctggagaa gagaccctca tctacagccc ttcaccagca ccacttccca cccccaactt
     3361 tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc ggtctcgatt
     3421 ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     3481 cttgtggaaa ggacgatact aaaacccttc ctcggaaccg acgcgttaag tcgacaatca
     3541 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3601 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3661 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3721 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3781 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3841 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3901 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3961 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4021 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4081 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     4141 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     4201 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     4261 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4321 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     4381 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     4441 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     4501 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4561 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4621 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     4681 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4741 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4801 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     4861 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     4921 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     4981 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     5041 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5101 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5161 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5221 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5281 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5341 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5401 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     5461 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5521 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5581 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5641 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5701 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5761 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5821 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5881 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5941 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6001 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6061 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6121 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6181 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6241 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6301 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6361 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6421 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6481 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6541 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6601 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6661 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6721 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6781 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6841 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6901 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6961 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7021 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7081 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7141 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7201 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7261 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7321 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7381 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7441 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7501 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7561 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7621 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     7681 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     7741 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     7801 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     7861 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     7921 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7981 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8041 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     8101 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     8161 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     8221 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     8281 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     8341 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     8401 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     8461 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     8521 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     8581 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     8641 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     8701 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     8761 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     8821 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     8881 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     8941 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     9001 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     9061 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     9121 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     9181 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     9241 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     9301 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     9361 ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac
     9421 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa
     9481 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9541 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9601 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9661 ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg
     9721 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     9781 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     9841 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     9901 acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     9961 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
    10021 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
    10081 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
    10141 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
    10201 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
    10261 cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga
    10321 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
    10381 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
    10441 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
    10501 c
//
