LOCUS       sequence-190450-       11281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of MATN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11281)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 11281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: MATN2_pLX307
FEATURES             Location/Qualifiers
     source          1..11281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4132..4156)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4164..4205
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4316..4904
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4369..4389)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4787..4798)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4976..5209
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5287..5408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5324..5343)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5378..5397
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5448..5583
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5603..5622)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5604..5622)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5632..5649)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5632..5648)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5641..5663)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5790..6245
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5877..5896)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6087..6108
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6271..6375
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6376..6444,6445..7236)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6594..6613)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7407..7995
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7896..7915
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8149..8166
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8283..8304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8319..8324,8325..8342,8343..8349)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8357..8373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8362..8384
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8381..8397
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8381..8397
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8416..8436
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8418..8436
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8464..8690
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8691..8871
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8918..9043
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9536..9769
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9954..9998
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10229..10439
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10251..10447
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10298..10433
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10479..11081
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10479..10498)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10975..10995
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11124..11241
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggaaaagat gctcgcaggc tgctttctgc tgatcctcgg acagatcgtc ctcctccctg
     1381 ccgaggccag ggagcggtca cgtgggaggt ccatctctag gggcagacac gctcggaccc
     1441 acccgcagac ggcccttctg gagagttcct gtgagaacaa gcgggcagac ctggttttca
     1501 tcattgacag ctctcgcagt gtcaacaccc atgactatgc aaaggtcaag gagttcatcg
     1561 tggacatctt gcaattcttg gacattggtc ctgatgtcac ccgagtgggc ctgctccaat
     1621 atggcagcac tgtcaagaat gagttctccc tcaagacctt caagaggaag tccgaggtgg
     1681 agcgtgctgt caagaggatg cggcatctgt ccacgggcac catgaccggg ctggccatcc
     1741 agtatgccct gaacatcgca ttctcagaag cagagggggc ccggcccctg agggagaatg
     1801 tgccacgggt cataatgatc gtgacagatg ggagacctca ggactccgtg gccgaggtgg
     1861 ctgctaaggc acgggacacg ggcatcctaa tctttgccat tggtgtgggc caggtagact
     1921 tcaacacctt gaagtccatt gggagtgagc cccatgagga ccatgtcttc cttgtggcca
     1981 atttcagcca gattgagacg ctgacctccg tgttccagaa gaagttgtgc acggcccaca
     2041 tgtgcagcac cctggagcat aactgtgccc acttctgcat caacatccct ggctcatacg
     2101 tctgcaggtg caaacaaggc tacattctca actcggatca gacgacttgc agaatccagg
     2161 atctgtgtgc catggaggac cacaactgtg agcagctctg tgtgaatgtg ccgggctcct
     2221 tcgtctgcca gtgctacagt ggctacgccc tggctgagga tgggaagagg tgtgtggctg
     2281 tggactactg tgcctcagaa aaccacggat gtgaacatga gtgtgtaaat gctgatggct
     2341 cctacctttg ccagtgccat gaaggatttg ctcttaaccc agatgaaaaa acgtgcacaa
     2401 agatagacta ctgtgcctca tctaatcatg gatgtcagca tgagtgtgtt aacacagatg
     2461 attcctattc ctgccactgc ctgaaaggct ttaccctgaa tccagataag aaaacctgca
     2521 gaaggatcaa ctactgtgca ctgaacaaac cgggctgtga gcatgagtgc gtcaacatgg
     2581 aggagagcta ctactgccgc tgccaccgtg gctacactct ggaccccaat ggcaaaacct
     2641 gcagccgagt ggaccactgt gcacagcagg accatggctg tgagcagctg tgtctgaaca
     2701 cggaggattc cttcgtctgc cagtgctcag aaggcttcct catcaacgag gacctcaaga
     2761 cctgctcccg ggtggattac tgcctgctga gtgaccatgg ttgtgaatac tcctgtgtca
     2821 acatggacag atcctttgcc tgtcagtgtc ctgagggaca cgtgctccgc agcgatggga
     2881 agacgtgtgc aaaattggac tcttgtgctc tgggggacca cggttgtgaa cattcgtgtg
     2941 taagcagtga agattcgttt gtgtgccagt gctttgaagg ttatatactc cgtgaagatg
     3001 gaaaaacctg cagaaggaaa gatgtctgcc aagctataga ccatggctgt gaacacattt
     3061 gtgtgaacag tgacgactca tacacgtgcg agtgcttgga gggattccgg ctcgctgagg
     3121 atgggaaacg ctgccgaagg aaggatgtct gcaaatcaac ccaccatggc tgcgaacaca
     3181 tttgtgttaa taatgggaat tcctacatct gcaaatgctc agagggattt gttctagctg
     3241 aggacggaag acggtgcaag aaatgcactg aaggcccaat tgacctggtc tttgtgatcg
     3301 atggatccaa gagtcttgga gaagagaatt ttgaggtcgt gaagcagttt gtcactggaa
     3361 ttatagattc cttgacaatt tcccccaaag ccgctcgagt ggggctgctc cagtattcca
     3421 cacaggtcca cacagagttc accctgagaa acttcaactc agccaaagac atgaaaaaag
     3481 ccgtggccca catgaaatac atgggaaagg gctctatgac tgggctggcc ctgaaacaca
     3541 tgtttgagag aagttttacc caaggagaag gggccaggcc cctttccaca agggtgccca
     3601 gagcagccat tgtgttcacc gacggacggg ctcaggatga cgtctccgag tgggccagta
     3661 aagccaaggc caatggtatc actatgtatg ctgttggggt aggaaaagcc attgaggagg
     3721 aactacaaga gattgcctct gagcccacaa acaagcatct cttctatgcc gaagacttca
     3781 gcacaatgga tgagataagt gaaaaactca agaaaggcat ctgtgaagct ctagaagact
     3841 ccgatggaag acaggactct ccagcagggg aactgccaaa aacggtccaa cagccaacag
     3901 tgcaacacag atatctgttt gaagaagaca atcttttacg gtctacacaa aagctttccc
     3961 attcaacaaa accttcagga agccctttgg aagaaaaaca cgatcaatgc aaatgtgaaa
     4021 accttataat gttccagaac cttgcaaacg aagaagtaag aaaattaaca cagcgcttag
     4081 aagaaatgac acagagaatg gaagccctgg aaaatcgcct gagatacaga tgcccaactt
     4141 tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc ggtctcgatt
     4201 ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     4261 cttgtggaaa ggacgatagt cgcctttatc gtatggggta acgcgttaag tcgacaatca
     4321 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4381 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4441 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4501 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4561 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4621 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4681 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     4741 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4801 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4861 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     4921 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     4981 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5041 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5101 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5161 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     5221 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5281 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5341 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5401 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     5461 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5521 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5581 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     5641 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5701 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5761 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     5821 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5881 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5941 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6001 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6061 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6121 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6181 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     6241 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6301 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6361 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6421 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6481 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6541 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6601 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6661 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6721 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6781 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6841 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6901 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6961 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7021 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7081 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7141 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7201 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7261 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7321 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7381 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7441 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7501 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7561 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7621 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7681 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7741 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7801 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7861 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7921 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7981 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8041 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8101 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8161 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     8221 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     8281 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     8341 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     8401 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     8461 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     8521 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     8581 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     8641 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     8701 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8761 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8821 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     8881 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     8941 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     9001 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     9061 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     9121 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     9181 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     9241 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     9301 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     9361 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     9421 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     9481 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     9541 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     9601 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     9661 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     9721 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     9781 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     9841 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     9901 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     9961 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
    10021 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
    10081 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
    10141 ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac
    10201 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa
    10261 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    10321 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    10381 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    10441 ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg
    10501 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
    10561 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
    10621 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
    10681 acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
    10741 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
    10801 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
    10861 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
    10921 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
    10981 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
    11041 cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga
    11101 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
    11161 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
    11221 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
    11281 c
//
