LOCUS       sequence-190456-        9847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of OXSM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: OXSM_pLX307
FEATURES             Location/Qualifiers
     source          1..9847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2479..2490
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(2698..2722)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2730..2771
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2882..3470
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2935..2955)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3353..3364)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3542..3775
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3853..3974
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3890..3909)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3944..3963
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4014..4149
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4169..4188)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4170..4188)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4198..4215)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4198..4214)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4207..4229)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4356..4811
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4443..4462)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4653..4674
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4837..4941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4942..5010,5011..5802)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5160..5179)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5973..6561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6462..6481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6715..6732
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6849..6870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6885..6890,6891..6908,6909..6915)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6923..6939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6928..6950
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6947..6963
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6947..6963
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6982..7002
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6984..7002
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7030..7256
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7257..7437
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7484..7609
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8102..8335
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8520..8564
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8795..9005
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8817..9013
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8864..8999
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9045..9647
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9045..9064)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9541..9561
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9690..9807
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgtccaactg cctgcaaaat ttcctgaaaa ttacaagcac tcgtcttcta tgttcaagat
     1381 tatgccaaca gttaagaagt aaaaggaagt ttttcggaac tgtgccaata tccagattgc
     1441 ataggcgagt tgtcattaca ggcattggct tagtgactcc tcttggtgtt ggaactcacc
     1501 tggtttggga tcgtcttatc ggaggagaga gtggaattgt ttcactggtt ggtgaagagt
     1561 ataagagtat cccttgcagt gttgctgctt atgtgccaag aggtagtgat gaaggtcagt
     1621 tcaatgaaca aaactttgtg tccaaatcag atatcaagtc catgtcttct cccaccatca
     1681 tggccattgg ggctgcagaa ttagccatga aggattctgg ctggcatcct cagtcagaag
     1741 ctgatcaagt ggctactggt gttgcaattg gcatgggaat gattcctctt gaagttgttt
     1801 ctgaaactgc tttgaatttt cagacaaaag gttacaataa agttagccca ttttttgtcc
     1861 ctaagattct ggtcaatatg gcagcaggcc aggtcagcat tcgatataaa ctcaagggcc
     1921 caaatcatgc agtatccaca gcctgtacca caggagctca tgctgtggga gactcattta
     1981 gatttatagc ccatggtgat gctgatgtga tggtggctgg aggtacagat tcttgtatta
     2041 gccctttatc tcttgctggg ttttccagag cccgggctct gagcacaaac tcagatccca
     2101 agttggcatg tcgaccattt catccaaaga gagatggttt tgtaatggga gaaggtgcag
     2161 ctgtgctggt gctggaagaa tatgaacatg ctgttcaaag aagagcccgg atctatgcag
     2221 aagttttggg ctatggactc tcaggtgatg ctggtcacat aactgcccct gatcctgaag
     2281 gagaaggtgc cttaaggtgt atggctgctg ctttaaaaga tgcaggtgtg cagcctgagg
     2341 agatatccta tatcaatgca catgctactt ccacaccatt gggagatgct gctgaaaaca
     2401 aagctatcaa acatctcttc aaagaccatg catatgccct tgcagtttcc tcaactaagg
     2461 gagcaacagg acatctgctg ggagctgcag gggcagtcga ggcagctttt accacattag
     2521 cttgttatta tcaaaaacta ccacctactt taaacctgga ttgttcggaa ccagaatttg
     2581 atctcaacta tgttccacta aaggcacagg aatggaaaac tgagaaaaga tttattggcc
     2641 tcaccaattc ctttggtttt ggtggtacta atgcaacact ttgtattgct ggactgtacc
     2701 caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct ctcctcggtc
     2761 tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt
     2821 atatatcttg tggaaaggac gaccgactca cgcagtgaaa caggatacgc gttaagtcga
     2881 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2941 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3001 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3061 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3121 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3181 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3241 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3301 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3361 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3421 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     3481 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     3541 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     3601 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3661 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3721 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     3781 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     3841 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3901 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3961 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     4021 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4081 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4141 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     4201 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     4261 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4321 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     4381 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4441 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4501 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4561 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4621 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4681 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4741 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4801 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4861 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4921 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4981 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5041 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5101 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5161 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5221 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5281 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5341 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5401 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5461 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5521 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5581 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5641 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5701 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5761 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5821 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5881 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5941 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6001 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6061 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6121 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6181 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6241 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6301 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6361 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6421 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6481 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6541 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6601 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6661 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6721 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6781 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6841 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6901 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6961 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     7021 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     7081 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     7141 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     7201 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     7261 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7321 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7381 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     7441 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     7501 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     7561 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     7621 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     7681 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     7741 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     7801 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     7861 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     7921 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     7981 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     8041 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     8101 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     8161 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     8221 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     8281 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     8341 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     8401 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     8461 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     8521 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     8581 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     8641 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     8701 tatcgtttca gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc
     8761 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg
     8821 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     8881 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8941 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9001 aggcttttgc aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc
     9061 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     9121 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     9181 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     9241 tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     9301 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     9361 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     9421 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     9481 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     9541 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     9601 aaggaccgcg cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc
     9661 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     9721 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     9781 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     9841 tggcgcc
//
