LOCUS       sequence-190457-       11791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of POLD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: POLD1_pLX307
FEATURES             Location/Qualifiers
     source          1..11791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2115..2126)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(4642..4666)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4674..4715
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4826..5414
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4879..4899)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5297..5308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5486..5719
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5797..5918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5834..5853)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5888..5907
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5958..6093
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6113..6132)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6114..6132)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6142..6159)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6142..6158)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6151..6173)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6300..6755
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6387..6406)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6597..6618
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6781..6885
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6886..6954,6955..7746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7104..7123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7917..8505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8406..8425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8659..8676
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8793..8814
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8829..8834,8835..8852,8853..8859)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8867..8883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8872..8894
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8891..8907
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8891..8907
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8926..8946
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8928..8946
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8974..9200
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9201..9381
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9428..9553
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10046..10279
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10464..10508
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10739..10949
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10761..10957
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10808..10943
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10989..11591
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10989..11008)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11485..11505
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11634..11751
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggatggcaa gcggcggcca ggcccagggc ccggggtgcc cccaaagcgg gcccgtgggg
     1381 gcctctggga tgatgatgat gcacctcggc catcccaatt cgaggaggac ctggcactga
     1441 tggaggagat ggaggcagaa cacaggctgc aggagcagga ggaggaggag ctgcagtcag
     1501 tcctggaggg ggttgcagac gggcaggtcc caccatcagc catagatcct cgctggcttc
     1561 ggcccacacc accagcgctg gacccccaga cagagcccct catcttccaa cagttggaga
     1621 ttgaccatta tgtgggccca gcacagcctg tgcctggggg gcccccacca tcccacggct
     1681 ccgtgcctgt gctccgcgcc ttcggggtca ccgatgaggg gttctctgtc tgctgccaca
     1741 tccacggctt cgctccctac ttctacaccc cagcgccccc tggtttcggg cccgagcaca
     1801 tgggtgacct gcaacgggag ctgaacttgg ccatcagccg ggacagtcgc ggggggaggg
     1861 agctgactgg gccggccgtg ctggctgtgg aactgtgctc ccgagagagc atgtttgggt
     1921 accacgggca cggcccctcc ccgttcctgc gcatcaccgt ggcgctgccg cgcctcgtgg
     1981 ccccggcccg ccgtctcctg gaacagggca tccgtgtggc aggcctgggc acgcccagct
     2041 tcgcgcccta cgaggccaac gtcgactttg agatccggtt catggtggac acggacatcg
     2101 tcggctgcaa ctggctggag ctcccagctg ggaaatacgc cctgaggctg aaggagaagg
     2161 ctacgcagtg ccagctggag gcggacgtgc tgtggtctga cgtggtcagt cacccaccgg
     2221 aagggccatg gcagcgcatt gcgcccttgc gcgtgctcag cttcgatatc gagtgcgccg
     2281 gccgcaaagg catcttccct gagcctgagc gggaccctgt catccagatc tgctcgctgg
     2341 gcctgcgctg gggggagccg gagcccttcc tacgcctggc gctcaccctg cggccctgtg
     2401 cccccatcct gggtgccaag gtgcagagct acgagaagga ggaggacctg ctgcaggcct
     2461 ggtccacctt catccgtatc atggaccccg acgtgatcac cggttacaac atccagaact
     2521 tcgaccttcc gtacctcatc tctcgggccc agaccctcaa ggtacaaaca ttccctttcc
     2581 tgggccgtgt ggccggcctt tgctccaaca tccgggactc ttcattccag tccaagcaga
     2641 cgggccggcg ggacaccaag gttgtcagca tggtgggccg cgtgcagatg gacatgctgc
     2701 aggtgctgct gcgggagtac aagctccgct cctacacgct caatgccgtg agcttccact
     2761 tcctgggcga gcagaaggag gacgtgcagc acagcatcat caccgacctg cagaatggga
     2821 acgaccagac ccgccgccgc ctggctgtgt actgcctgaa ggatgcctac ctgccactgc
     2881 ggctgctgga gcggctcatg gtgctggtga acgccgtgga gatggcgagg gtcactggcg
     2941 tgcccctcag ctacctgctc agtcgtggcc agcaggtcaa ggtcgtatcc cagctgttgc
     3001 ggcaggccat gcacgagggg ctgctgatgc ccgtggtgaa gtcagagggc ggcgaggact
     3061 acacgggagc cactgtcatc gagcccctca aagggtacta cgacgtcccc atcgccaccc
     3121 tggacttctc ctcgctgtac ccgtccatca tgatggccca caacctgtgt tacaccacgc
     3181 tccttcggcc cgggactgca cagaaactgg gcctgactga ggatcagttc atcaggaccc
     3241 ccaccgggga cgagtttgtg aagacctcag tgcggaaggg gctgctgccc cagatcctgg
     3301 agaacctgct cagtgcccgg aagagggcca aggccgagct ggccaaggag acagaccccc
     3361 tccggcgcca ggtcctggat ggacggcagc tggcgctgaa ggtgagcgcc aactccgtat
     3421 acggcttcac tggcgcccag gtgggcaagt tgccgtgcct ggagatctca cagagcgtca
     3481 cggggttcgg acgtcagatg atcgagaaaa ccaagcagct ggtggagtct aagtacacag
     3541 tggagaatgg ctacagcacc agtgccaagg tggtgtatgg tgacactgac tccgtcatgt
     3601 gccgattcgg cgtgtcctcg gtggctgagg cgatggccct ggggcgggag gccgcggact
     3661 gggtgtcagg tcacttcccg tcgcccatcc ggctggagtt tgagaaggtc tacttcccat
     3721 acctgcttat cagcaagaag cgctacgcgg gcctgctctt ctcctcccgg cccgacgccc
     3781 acgaccgcat ggactgcaag ggcctggagg ccgtgcgcag ggacaactgc cccctcgtgg
     3841 ccaacctggt cactgcctca ctgcgccgcc tgctcatcga ccgagaccct gagggcgcgg
     3901 tggctcacgc acaggacgtc atctcggacc tgctgtgcaa ccgcatcgat atctcccagc
     3961 tggtcatcac caaggagctg acccgcgcgg cctccgacta tgccggcaag caggcccacg
     4021 tggagctggc cgagaggatg aggaagcggg accccgggag tgcgcccagc ctgggcgacc
     4081 gcgtccccta cgtgatcatc agtgccgcca agggtgtggc cgcctacatg aagtcggagg
     4141 acccgctgtt cgtgctggag cacagcctgc ccattgacac gcagtactac ctggagcagc
     4201 agctggccaa gcccctcctg cgcatcttcg agcccatcct gggcgagggc cgtgccgagg
     4261 ctgtgctact gcggggggac cacacgcgct gcaagacggt gctcacgggc aaggtgggcg
     4321 gcctcctggc cttcgccaaa cgccgcaact gctgcattgg ctgccgcaca gtgctcagcc
     4381 accagggagc cgtgtgtgag ttctgccagc cccgggagtc tgagctgtat cagaaggagg
     4441 tatcccatct gaatgccctg gaggagcgct tctcgcgcct ctggacgcag tgccagcgct
     4501 gccagggcag cctgcacgag gacgtcatct gcaccagccg ggactgcccc atcttctaca
     4561 tgcgcaagaa ggtgcggaag gacctggaag accaggagca gctcctgcgg cgcttcggac
     4621 cccctggacc tgaggcctgg tgcccaactt tcttgtacaa agtggttgat atcggtaagc
     4681 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt ccgtaacttg
     4741 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacccc ctattcgaat
     4801 caaacgggct acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     4861 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4921 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4981 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5041 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5101 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5161 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5221 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5281 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5341 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5401 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     5461 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5521 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5581 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5641 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5701 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     5761 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     5821 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5881 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     5941 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6001 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6061 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     6121 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     6181 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     6241 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     6301 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6361 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6421 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6481 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6541 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6601 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6661 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6721 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     6781 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6841 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6901 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6961 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7021 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7081 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     7141 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7201 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7261 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7321 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7381 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7441 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7501 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7561 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7621 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7681 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7741 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7801 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7861 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7921 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7981 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8041 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8101 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8161 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8221 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8281 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8341 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8401 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8461 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8521 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8581 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8641 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8701 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8761 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8821 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     8881 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     8941 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     9001 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     9061 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     9121 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     9181 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     9241 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9301 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9361 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     9421 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     9481 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     9541 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     9601 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     9661 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     9721 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     9781 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     9841 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     9901 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     9961 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    10021 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    10081 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    10141 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    10201 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    10261 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    10321 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    10381 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    10441 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    10501 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    10561 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    10621 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
    10681 ccatgcattg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    10741 aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac
    10801 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10861 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    10921 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg
    10981 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
    11041 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
    11101 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
    11161 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca
    11221 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
    11281 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
    11341 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
    11401 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
    11461 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
    11521 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
    11581 gtgccagtta acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa
    11641 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    11701 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    11761 tacagggaca gcagagatcc actttggcgc c
//
