LOCUS       sequence-190494-        6563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-mediated expression of ArchT under the CamKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, 
            Baratta MV, Winkle J, Desimone R, Boyden ES
  TITLE     A high-light sensitivity optical neural silencer: development and 
            application to optogenetic control of non-human primate cortex.
  JOURNAL   Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13.
  PUBMED    21811444
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Syst 
            Neurosci."; date: "2011"; volume: "5"; pages: "18. Epub 2011 Apr 13"
COMMENT     Sequence Label: pAAV-CamKII-ArchT-GFP (PV2527)
FEATURES             Location/Qualifiers
     source          1..6563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   36..165
                     /label=AAV2 ITR
     promoter        238..1526
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      1536..1545
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2301..2303,2304..3017)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2301..2303,2304..3014)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2343..2364)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2604..2623)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2951..2972
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3042..3630
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3095..3115)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3513..3524)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3673..3794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3684..3703)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3738..3757
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3956..4085
                     /label=AAV2 ITR
     rep_origin      complement(4322..4910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4402..4421)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5081..5872,5873..5941))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5704..5723
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5942..6046)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6072..6527)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6209..6230)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6421..6440
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcgatcggt gcgggcctct tcgctattac gccagctgcg cgctcgctcg ctcactgagg
       61 ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc
      121 gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag ttaatgatta
      181 acccgccatg ctacttatct acgtagccat gctctaggaa gatcgtaggt taattaacat
      241 tatggcctta ggtcacttca tctccatggg gttcttcttc tgattttcta gaaaatgaga
      301 tgggggtgca gagagcttcc tcagtgacct gcccagggtc acatcagaaa tgtcagagct
      361 agaacttgaa ctcagattac taatcttaaa ttccatgcct tgggggcatg caagtacgat
      421 atacagaagg agtgaactca ttagggcaga tgaccaatga gtttaggaaa gaagagtcca
      481 gggcagggta catctacacc acccgcccag ccctgggtga gtccagccac gttcacctca
      541 ttatagttgc ctctctccag tcctaccttg acgggaagca caagcagaaa ctgggacagg
      601 agccccagga gaccaaatct tcatggtccc tctgggagga tgggtgggga gagctgtggc
      661 agaggcctca ggaggggccc tgctgctcag tggtgacaga taggggtgag aaagcagaca
      721 gagtcattcc gtcagcattc tgggtctgtt tggtacttct tctcacgcta aggtggcggt
      781 gtgatatgca caatggctaa aaagcaggga gagctggaaa gaaacaagga cagagacaga
      841 ggccaagtca accagaccaa ttcccagagg aagcaaagaa accattacag agactacaag
      901 ggggaaggga aggagagatg aattagcttc ccctgtaaac cttagaaccc agctgttgcc
      961 agggcaacgg ggcaatacct gtctcttcag aggagatgaa gttgccaggg taactacatc
     1021 ctgtctttct caaggaccat cccagaatgt ggcacccact agccgttacc atagcaactg
     1081 cctctttgcc ccacttaatc ccatcccgtc tgttaaaagg gccctatagt tggaggtggg
     1141 ggaggtagga agagcgatga tcacttgtgg actaagtttg ttcgcatccc cttctccaac
     1201 cccctcagta catcaccctg ggggaacagg gtccacttgc tcctgggccc acacagtcct
     1261 gcagtattgt gtatataagg ccagggcaaa gaggagcagg ttttaaagtg aaaggcaggc
     1321 aggtgttggg gaggcagtta ccggggcaac gggaacaggg cgtttcggag gtggttgcca
     1381 tggggacctg gatgctgacg aaggctcgcg aggctgtgag cagccacagt gccctgctca
     1441 gaagccccaa gctcgtcagt caagccggtt ctccgtttgc actcaggagc acgggcaggc
     1501 gagtggcccc tagttctggg ggcagcgggg gatccgccac catggacccc atcgctctgc
     1561 aggcaggata cgacctgctg ggcgacggaa ggccagagac cctgtggctg ggaatcggaa
     1621 ccctgctgat gctgatcggc accttctact tcatcgtgaa gggctggggc gtgaccgaca
     1681 aggaggccag ggagtactac agcatcacaa tcctggtgcc cggcatcgcc agcgccgcct
     1741 acctgagcat gttcttcggc atcggcctga ccgaggtgac cgtggccggc gaggtgctgg
     1801 acatctacta cgccagatac gccgactggc tgttcaccac ccctttgctt ctgctcgacc
     1861 tggccctgct ggctaaggtg gacagggtga gcatcggaac cctggtggga gtggacgccc
     1921 tgatgatcgt gaccggcctg atcggcgccc tgagccacac cccactggct aggtacagct
     1981 ggtggctgtt cagcaccatc tgcatgatcg tggtgctgta cttcctggct accagcctga
     2041 gggctgctgc taaggagagg ggaccagagg tggctagcac cttcaacacc ctgaccgccc
     2101 tggtgctggt gctgtggacc gcctacccca tcctgtggat catcggaacc gagggagctg
     2161 gagtggtggg actgggaatc gagaccctgc tgttcatggt gctggacgtg accgccaaag
     2221 tgggcttcgg cttcatcctg ctgaggagca gggccatcct gggcgacacc gaggcccccg
     2281 agccccgacc ggtagtagca gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     2341 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     2401 agggcgatgc cacctacggc aagctgaccc tgaagttcat ttgcaccacc ggcaagctgc
     2461 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     2521 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     2581 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     2641 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2701 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     2761 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     2821 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     2881 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     2941 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     3001 acgagctgta caagtaagaa ttcgatatca agcttatcga taatcaacct ctggattaca
     3061 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3121 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3181 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3241 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     3301 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     3361 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     3421 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     3481 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     3541 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     3601 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacccggg cggccgcttc
     3661 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     3721 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3781 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     3841 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     3901 ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa
     3961 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     4021 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     4081 cgcagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4141 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4201 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4261 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4321 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4381 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4441 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4501 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4561 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4621 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4681 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4741 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4801 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4861 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4921 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4981 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5041 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5101 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5161 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5221 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5281 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5341 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5401 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5461 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     5521 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     5581 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5641 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5701 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5761 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     5821 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5881 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5941 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6001 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6061 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     6121 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     6181 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     6241 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     6301 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     6361 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     6421 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     6481 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     6541 caggctgcgc aactgttggg aag
//
