LOCUS       sequence-190498-        2972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BphP1-PpsR2 chromophore plasmid; ho1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ong NTX, Olson EJ, Tabor JJ
  TITLE     Engineering an E. coli near-infrared light sensor.
  JOURNAL   ACS Synth Biol. 2017 Nov 1. doi: 10.1021/acssynbio.7b00289.
  PUBMED    29091422
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Nov 1. doi: 10.1021/acssynbio.7b00289."
COMMENT     Sequence Label: pNO41
FEATURES             Location/Qualifiers
     source          1..2972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      50..595
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     496..515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     712..729
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        936..970
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     RBS             987..998
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1736..1807
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1823..1850
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2011..2802
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      2814..2908
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 attgcactga aatctagtaa tattttatct gattaataag atgatcttct tgagatcgtt
       61 ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt
      121 cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt
      181 caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct
      241 ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac
      301 tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata
      361 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa
      421 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
      481 gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
      541 ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg
      601 ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct
      661 ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca
      721 gtgagcgagg aagcggaata tatcccctag gtggagtctg gcctcaaata caaagtctgc
      781 aaagtctcga aatattaaac cagagaataa ataattaaac gccaaaatat ttctagcaaa
      841 aaccccagtt attaaaccgc ctaagtcccc caggaaaggg ggatataaca gtatagattt
      901 tgtcagcctt cagcttggct ttaccgtcaa aaaaatttac ggctagctca gccctaggta
      961 ttatgctagc tcacacagaa ttcattaaag aggagaaagg taccatgagt gtcaacttag
     1021 cttcccagtt gcgggaaggg acgaaaaaat cccactccat ggcggagaac gtcggctttg
     1081 tcaaatgctt cctcaagggc gttgtcgaga aaaattccta ccgtaagctg gttggcaatc
     1141 tctactttgt ctacagtgcc atggaagagg aaatggcaaa atttaaggac catcccatcc
     1201 tcagccacat ttacttcccc gaactcaacc gcaaacaaag cctagagcaa gacctgcaat
     1261 tctattacgg ctccaactgg cggcaagaag tgaaaatttc tgccgctggc caagcctatg
     1321 tggaccgagt ccggcaagtg gccgctacgg cccctgaatt gttggtggcc cattcctaca
     1381 cccgttacct gggggatctt tccggcggtc aaattctcaa gaaaattgcc caaaatgcca
     1441 tgaatctcca cgatggtggc acagctttct atgaatttgc cgacattgat gacgaaaagg
     1501 cttttaaaaa tacctaccgt caagctatga atgatctgcc cattgaccaa gccaccgccg
     1561 aacggattgt ggatgaagcc aatgacgcct ttgccatgaa catgaaaatg ttcaacgaac
     1621 ttgaaggcaa cctgatcaag gcgatcggca ttatggtgtt caacagcctc acccgtcgcc
     1681 gcagtcaagg cagcaccgaa gttggcctcg ccacctccga aggctagcca ggcatcaaat
     1741 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1801 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggta
     1861 ccagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
     1921 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct
     1981 gatcaagaga caggatgagg atcgtttcgc atgagggaag cggtgatcgc cgaagtatcg
     2041 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     2101 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     2161 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     2221 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     2281 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     2341 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     2401 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     2461 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     2521 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     2581 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     2641 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     2701 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     2761 cactacgtga aaggcgagat caccaaggta gtcggcaaat aagagctcgc ttggactcct
     2821 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
     2881 ccgccgggcg ttttttattg gtgagaatcc aagcactagt aacaacttat atcgtatggg
     2941 gctgacttca ggtgctacat ttgaagagat aa
//
