LOCUS       sequence-190500-        4142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BphP1-PpsR2 reporter plasmid; PBr_crtE-sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ong NTX, Olson EJ, Tabor JJ
  TITLE     Engineering an E. coli near-infrared light sensor.
  JOURNAL   ACS Synth Biol. 2017 Nov 1. doi: 10.1021/acssynbio.7b00289.
  PUBMED    29091422
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Nov 1. doi: 10.1021/acssynbio.7b00289."
COMMENT     Sequence Label: pNO4
FEATURES             Location/Qualifiers
     source          1..4142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..799
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    826..846
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(930..947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1209..2159)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2207..2429
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2921..3015)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3045..3836,3837..3905))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3668..3687
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3906..4010)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4075..4093
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4097..4140)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gacgcaggaa atgtctagca tgatttacag tataggtgta agctgtaatt tacatgtaaa
       61 gaaataaaaa gggaggttac aacctatgcg taaaggcgaa gagctgttca ctggtgtcgt
      121 ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg tgcgtggcga
      181 gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta ctactggtaa
      241 actgccggta ccttggccga ctctggtaac gacgctgact tatggtgttc agtgctttgc
      301 tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc cggaaggcta
      361 tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc gtgcggaagt
      421 gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg actttaaaga
      481 agacggcaat atcctgggcc ataagctgga atacaatttt aacagccaca atgtttacat
      541 caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc acaacgtgga
      601 ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg gtgatggtcc
      661 tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta aagatccgaa
      721 cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg
      781 tatggatgaa ctgtacaaat gatgagcccg tatttcgcgt aaggatacta gtagcggccg
      841 ctgcagtccg gcaaaaaaac gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
      901 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      961 cggctgcggc gagcggtatc agctcactca aaggcggtaa tctcgaggtt acattgtcga
     1021 tctgttcatg gtgaacagct ttaaatgcac caaaaactcg taaaagctct gatgtatcta
     1081 tcttttttac accgttttca tctgtgcata tggacagttt tccctttgat atctaacggt
     1141 gaacagttgt tctacttttg tttgttagtc ttgatgcttc actgatagat acaagagcca
     1201 taagaacctc agatccttcc gtatttagcc agtatgttct ctagtgtggt tcgttgtttt
     1261 tgcgtgagcc atgagaacga accattgaga tcatgcttac tttgcatgtc actcaaaaat
     1321 tttgcctcaa aactggtgag ctgaattttt gcagttaaag catcgtgtag tgtttttctt
     1381 agtccgttac gtaggtagga atctgatgta atggttgttg gtattttgtc accattcatt
     1441 tttatctggt tgttctcaag ttcggttacg agatccattt gtctatctag ttcaacttgg
     1501 aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca ccaatttcat attgctgtaa
     1561 gtgtttaaat ctttacttat tggtttcaaa acccattggt taagcctttt aaactcatgg
     1621 tagttatttt caagcattaa catgaactta aattcatcaa ggctaatctc tatatttgcc
     1681 ttgtgagttt tcttttgtgt tagttctttt aataaccact cataaatcct catagagtat
     1741 ttgttttcaa aagacttaac atgttccaga ttatatttta tgaatttttt taactggaaa
     1801 agataaggca atatctcttc actaaaaact aattctaatt tttcgcttga gaacttggca
     1861 tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag gattcctgat ttccacagtt
     1921 ctcgtcatca gctctctggt tgctttagct aatacaccat aagcattttc cctactgatg
     1981 ttcatcatct gagcgtattg gttataagtg aacgataccg tccgttcttt ccttgtaggg
     2041 ttttcaatcg tggggttgag tagtgccaca cagcataaaa ttagcttggt ttcatgctcc
     2101 gttaagtcat agcgactaat cgctagttca tttgctttga aaacaactaa ttcagacata
     2161 catctcaatt ggtctaggtg attttaatca ctataccaat tgagatgggc tagtcaatga
     2221 taattacatg tccttttcct ttgagttgtg ggtatctgta aattctgcta gacctttgct
     2281 ggaaaacttg taaattctgc tagaccctct gtaaattccg ctagaccttt gtgtgttttt
     2341 tttgtttata ttcaagtggt tataatttat agaataaaga aagaataaaa aaagataaaa
     2401 agaatagatc ccagccctgt gtataactca ctactttagt cagttccgca gtattacaaa
     2461 aggatgtcgc aaacgctgtt tgctcctcta caaaacagac cttaaaaccc taaaggctta
     2521 agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc cttttgtctc cgaccatcag
     2581 gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt
     2641 gaatgggggt aaatggcact acaggcgcct tttatggatt catgcaagga aactacccat
     2701 aatacaagaa aagcccgtca cgggcttctc agggcgtttt atggcgggtc tgctatgtgg
     2761 tgctatctga ctttttgctg ttcagcagtt cctgccctct gattttccag tctgaccact
     2821 tcggattatc ccgtgacagg tcattcagac tggctaatgc acccagtaag gcagcggtat
     2881 catcaacagg cttacccgtc ttactgtccc tagtgcttgg attctcacca ataaaaaacg
     2941 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     3001 ctatcaacag gagtccaagc gagctcgtaa acttggtctg acagttacca atgcttaatc
     3061 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3121 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3181 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3241 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3301 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3361 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3421 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3481 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3541 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3601 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3661 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3721 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3781 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3841 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3901 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3961 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4021 atggtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     4081 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     4141 tg
//
