LOCUS       sequence-190537-       10078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NLS-GFP-lacZ leu1 insertion vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova
            L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost A
  TITLE     LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in 
            fission yeast and human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 
            10.1073/pnas.1613916114. Epub 2017 Feb 27.
  PUBMED    28242692
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1613916114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-03-14- 14"; volume: "114"; issue: "11"; pages: 
            "E2166-E2175"
COMMENT     Sequence Label: pMGF173
FEATURES             Location/Qualifiers
     source          1..10078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     212..229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    346..367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(382..387,388..405,406..412)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    420..436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     425..447
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     444..460
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     444..460
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1734..1754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1767..2477
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1794..1822)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2417..2437
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      5622..5809
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5677..5701)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7446..7463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7446..7462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7455..7477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7675..8130
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7762..7781)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7972..7993
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8147..8166)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8266..8288
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8326..8344)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8412..8516
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8517..8585,8586..9377)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8735..8754)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9548..10078,1..58)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10037..10056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      121 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      181 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      241 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      301 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      361 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      421 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      481 atgcctgcag gtcgatcgac tctagaggat cagaaaatta tcgccataaa agacagaata
      541 agtcatcagc ggttgtttca tttcctatat ttttttttta tttttttatt ttttaataag
      601 ggaaaattta acgtctaagg atacagaaga ttgttagcac attaaagtaa taaaggctta
      661 agtagtaagt gccttagcat gttattgtat ttcaaaggac ataatctaaa ataataacaa
      721 tatcatttct cacaagttat tcaattttct ttttttttct aataatatca agaatgtatt
      781 atttgtttga cataagtcaa ctaatttatt taatatgctg gattaatctt gcagacatgt
      841 aaattaacaa gttttagtca aataacgttg aagtttcaat gaactcaaat aatttctctt
      901 tttttttata taaccataat ctgatttata ttttccgcag ggatcaactg aagttatgac
      961 atttggattg gatcacttat aaccttggtc gccaaataat acaaaaatca gcgttataaa
     1021 acaaagaagg tttttgttaa gaaattaatc ctctttcttg ataagaaagt tgaaccgaaa
     1081 ttgcagatac tgatatatga aaataatacc cacaattttg ggaatagcgc aagcctcaat
     1141 ttaaacaata ggtgaggaca catgataatg acctcaatga ttgttagaag aaaagagcct
     1201 cattacaaaa tcgaaaaatg aatggttggg tacaagtttc caaaacatgg taaagtggac
     1261 tttgcgtatg agacgtaaat agaaaaaaac acttgttata tgttttctag aattattgtt
     1321 gtctctttat ggttggatga tgcaaaatag taatttcggt tagttgctgt aaaacaccac
     1381 gagacaaata gatatggata tttattaaat caggaaaaac gtaactctcg gctactggat
     1441 ggttcagtca cccaacgatt actggggaga gaaaacaggg caaaagcaaa gcttaaagga
     1501 atccgattgt cattcggcaa tgtgcagcga aactaaaaac cggataatgg acctgttaat
     1561 cgaaacattg aagatatata aaggaagagg aatcctggca tatcatcaat tgaataagtt
     1621 gaattaatta tttcaatctc attctcactt tctgacttat agtcgctttg ttaaagctag
     1681 cgtcgacagg cctggatccg gatatcaaca agtttgtaca aaaaagcatg agtccaaaga
     1741 agaagagaaa agtcgaagac ccacgcagta aaggagaaga acttttcact ggagttgtcc
     1801 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     1861 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     1921 tacctgttcc atggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa
     1981 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
     2041 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     2101 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     2161 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     2221 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     2281 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     2341 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     2401 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     2461 tggatgaact atacaaaaga tccggagctt ggctgttgcc cgtctcactg gtgaaaagaa
     2521 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2581 tgcagctggc acgacaggtt tcccgactta atcgccttgc agcacatccc cctttcgcca
     2641 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     2701 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg
     2761 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt
     2821 acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg ccgtttgttc
     2881 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac
     2941 aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca
     3001 acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg
     3061 catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg agtgacggca
     3121 gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc
     3181 tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt
     3241 tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac
     3301 gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg
     3361 gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg
     3421 tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac
     3481 tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg
     3541 tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta
     3601 accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg
     3661 atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc
     3721 atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata
     3781 ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg
     3841 cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca
     3901 tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga
     3961 tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg
     4021 ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg
     4081 tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc
     4141 tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg
     4201 cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc
     4261 tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata
     4321 cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc
     4381 cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa
     4441 ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga
     4501 tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac
     4561 aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct
     4621 ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca
     4681 gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc
     4741 acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc
     4801 gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac
     4861 aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg
     4921 taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc
     4981 attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc
     5041 tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa
     5101 cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg
     5161 atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg
     5221 taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt
     5281 ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa
     5341 acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact
     5401 tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc
     5461 tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg
     5521 gcgacgactc ctggagcccg tcagtatcgg cggaattaca gctgagcgcc ggtcgctacc
     5581 attaccagtt ggtctggtgt caaaaataaa cttctaaata agcgaatttc ttatgattta
     5641 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact
     5701 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc
     5761 tttctcaggt atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta
     5821 gggataacag ggtaatatac tagtaagatg attcttttat cgtcgataac gatgaggatg
     5881 tagacgatta tgatgagagt gattagcttc taaaatttgg tgttttcaaa cgctatttac
     5941 agtacaatct gaaaaaattg gatgaacatt gtaaatggta ggatacgagt tccaattaat
     6001 gtatttttta agtattacac ttagcttatt aattttatat atacattcta cctctgtggg
     6061 tttaaagaca actcgtacac atactcaaat tatttatgaa cattataatg catattcgta
     6121 ctgtctatta aacatttttt tgacattaaa aatttcgttt actaacgtag aaagcgctga
     6181 taattgaggc tactatttta atatctagaa acaaagattc attaattaat taagtaagct
     6241 ccaggatact tgtatatttc gttaaaaaag cgcaatttca acaattccta tgaacatcca
     6301 tgatttcatc acaaaatcaa acatatcagg atttaacgtg aattatttgc tttcttgtga
     6361 ttttcatctg atttctggtc atttacgtta ctgtaaacaa gctttttaaa aaaaggattt
     6421 tttacgcaat attatcgata tcccaatctg tagtataaaa agtgcaacag tgttgagttt
     6481 cccgaaacca ggcatttttg cggtagggtc tgaaactttc acgaaaccat ttcacaacaa
     6541 aacttcttca acaacatgtg tgcaaagaaa atcgtcgtct taccaggaga ccatattggc
     6601 cctgaaattg ttgcttctgc cttggaggtt ttgaaagtcg ttgagaagaa gcgacctgag
     6661 ttaaaactcg agtttgaaga acacaagatt ggaggtgcct ctattgatgc ctatggaacc
     6721 cctttgactg acgagactgt gaaggcttgt ttggaagctg acggtgttct tttgggtgcc
     6781 gttggtggtc ctgaatggac caaccccaat tgtcgtcctg agcaaggttt attgaagctt
     6841 cgtaagagta tgggtgtttg ggccaacctt cgaccttgca actttgccag caagtcttta
     6901 gtcaagtaca gccctttgaa gcctgaaatc gttgaaggtg tcgatttttg tgttgtacga
     6961 gaacttactg gaggttgtta ctttggtgag cgcactgagg acaacggatc gggttatgct
     7021 atggacactt ggccttacag tttggaagaa gtttctcgta ttgctcgttt ggcggccgct
     7081 tagctgaaac ttccaaccct cctgctcccg tcacattact cgacaaagct aatgttttgg
     7141 caacttctcg tctttggcgc aagactgtag ctaagatttt taaggaggaa tatcctcacc
     7201 ttaccttaaa aaaccagctc attgactccg cagccatgct tttggtcaag agccctcgta
     7261 cacttaacgg tgttgttttg actgacaact tgtttggtga cattatttca gatgaggctt
     7321 ctgtcattcc tggtagcttg ggccttttgc cttctgcctc cctttccggt gtggtaggaa
     7381 aatcagaaga aaaggttcat tgtttggttg agcccattca cggtagcgct cccgagctcg
     7441 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7501 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7561 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     7621 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     7681 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7741 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7801 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7861 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     7921 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7981 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     8041 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8101 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     8161 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     8221 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     8281 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     8341 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     8401 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8461 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8521 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8581 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8641 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8701 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8761 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8821 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8881 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8941 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     9001 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     9061 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9121 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9181 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9241 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9301 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9361 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9421 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9481 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9541 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9601 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9661 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9721 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9781 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9841 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9901 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9961 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10021 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgcca
//
