LOCUS       sequence-19055-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Zhang Y, Feng X, We R, Derynck R
  TITLE     Receptor-associated Mad homologues synergize as effectors of the 
            TGF-beta response.
  JOURNAL   Nature. 1996 Sep 12. 383(6596):168-72.
  PUBMED    8774881
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1996 Sep 12. 383(6596):168-72."
COMMENT     Sequence Label: pRK Smad3 deltaC Flag
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    66..200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(90..109)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     144..163
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     regulatory      204..213
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(225..248)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtacctaacc aagttcctct ttcagaggtt atttcaggcc atggtgctgc gccgaattaa
       61 ttcccgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
      121 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
      181 taagctgcaa taaacaagtt gggccatggc ggccaagctt atcacttgtc gtcgtcgtcc
      241 ttgtagtcca tgtcgactgc tccccagccc ttcacgaagc tcatgcggat ggtgcacatt
      301 cgggtcaact ggtagacagc ctcaaagccc tggttgaccg actgggccag gagggcagcg
      361 aactcctggt tgttgaagat cttcaggttg catcctggtg ggatcttgca gacggtggcc
      421 gggtgccagc catagcgctg gttacagttg ggagactgga caaaaatagc gctgtcactg
      481 aggcactctg cgaagacctc ccctccgatg tagtagagcc gcacgcctct tccgatgtgt
      541 ctccgtgtca gctccactgc tgcattcctg ttgacattgg agagcagccc taggcagaag
      601 cgctccgaat tggaggggtc ggtgaagcca tccacagtca tggatggctg cgaggcgtgg
      661 aatgtctccc cgacgcgctg gttcagctcg tagta
//
