LOCUS       sequence-190559-       10027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ish1 mCherry insertion cassette with ura4+ gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova
            L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost A
  TITLE     LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in 
            fission yeast and human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 
            10.1073/pnas.1613916114. Epub 2017 Feb 27.
  PUBMED    28242692
REFERENCE   2  (bases 1 to 10027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1613916114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-03-14- 14"; volume: "114"; issue: "11"; pages: 
            "E2166-E2175"
COMMENT     Sequence Label: pMGF172
FEATURES             Location/Qualifiers
     source          1..10027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(489..494,495..512,513..519)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    527..543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     551..567
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     551..567
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             563..1090
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(583..600)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(583..599)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(592..614)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(1192..1197,1198..1213,1214..1220)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1228..1244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1267..1920
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1878..1900
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1927..1950
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2545..3255
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2690..2708)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2958..2977
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      3276..3463
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3331..3355)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(3945..4739)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      5348..6131
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(7086..7108)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7253..7271)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7339..7443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7444..7512,7513..8304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7662..7681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8475..9063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8964..8983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9217..9234
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        9307..9384
                     /gene="lacI"
                     /label=lacI promoter
     CDS             join(9385..10027,1..440)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9404..9423)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg
       61 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc
      121 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg
      181 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc
      241 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
      301 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac cacgctagcg cccaatacgc
      361 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
      421 gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac tcattaggca
      481 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      541 caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca
      601 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      661 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      721 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
      781 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
      841 gcacggttac gatgcgccca tctacaccaa cgtgacctat cccattacgg tcaatccgcc
      901 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
      961 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttggaatta gcttatcgac
     1021 tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg
     1081 caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg
     1141 ttttttgcgc cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatt
     1201 aatcatcggc tcgtataatg tgtggaattg tgagcggata acaatttcac acaggaaaca
     1261 gtattcatgt cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga
     1321 cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt
     1381 gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat
     1441 attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag
     1501 cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg
     1561 gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc
     1621 aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt
     1681 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct
     1741 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt
     1801 tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat
     1861 atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa
     1921 tcggatctgg aagttctgtt ccaggggccc ctggctgttc ctaattgggc tgcttatttt
     1981 gatactttga gctccaaaga gctttctgcc tggattaagg aatacaaaaa acattaccat
     2041 ggaaaacttc acccatccaa agatcgtgaa catcttttcc aaaatgcctg taatatttat
     2101 cgcgctattg ctgaaaaggc tgacaaatct gttcttcaat cgattcaatc aaagtttcct
     2161 aaggcaactg ttcccggtta tgactcttgg tctaatgaag atctaaagtc tgcactcaag
     2221 gaatatggtg acagtattgg taaggtgttt aatcgtaaag atgctattga acgcttaaag
     2281 cgtcatgata ttttattcta tggtcctgtt gttgccgaca aggctaaatc aggagtctct
     2341 ggcttccttc gacgtgttgc ttcttttatg ggatttggca ctcgcgacgt agctgaaatt
     2401 aagctaggag aaaacattca aaaggtaaaa gatactgcct ctaaagcctc atctgctgta
     2461 tcttcagcag gcgactatgt cgaaactaat gtaaagaaag cccaaagggt tttgggagga
     2521 acgggcggta gtggaggcac tggtatggtg agcaagggcg aggaggataa catggccatc
     2581 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     2641 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     2701 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     2761 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     2821 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     2881 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     2941 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     3001 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     3061 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     3121 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     3181 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     3241 gagctgtaca agtgaggcgc gccacttcta aataagcgaa tttcttatga tttatgattt
     3301 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     3361 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     3421 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata
     3481 acagggtaat atagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac
     3541 atggaggccc agaatacaac accaatgttt ataaccaagt tttatcttgt ttgtctacat
     3601 ggtattttac attcatctac atacatcttt cattggcttt gtacatagtt atcattacaa
     3661 gtctaaaaaa attcactctt ttcttattca atgtcaatcc aagagaaaag attgtggtaa
     3721 tgttgtagga gcatgtttaa taaattacta tagcaaatta ctttttattc ccaaggtgtt
     3781 tatctataat agttaatatt ttagtcgcta cataaaattt taccaaagag tacttgtata
     3841 ctaattctaa atgccttctg acataaaacg cctaggaaaa caaacgcaaa caaggcatcg
     3901 actttttcaa taaccaacca aaaaaatttt acattagtct ttttttaatg ctgagaaagt
     3961 ctttgctgat atgccttcca accagcttct ctatatctct tggcttcgac aacaggatta
     4021 cgaccagctc catagactcc acgaccaaca atgatgatgt cgctaccgca gtttacaatc
     4081 acttcttcag gagtacgata ttgctgtccc agcccgtctc ctttaacatc caagccgata
     4141 ccaggggaca tagttatgta gtcgctttga aggttaggaa atcgacgacc agctataaag
     4201 ccaaagcaaa aatcggtatg cttctcaaac cattctaagg ttttctctgt gtaggaacca
     4261 gtagccaaag agcctttgga agacatttca gccaaaagca agagaccacg tcccaaaggt
     4321 aaaccaactt ctttgaggcc ttgtataata ccctcgcctg gcactgtatg gcaatttgtg
     4381 atatgagccc aagaagcaat tttgtacaca ccagatgcat attgtagctt gacggtattt
     4441 ccaatgtctg cgaatttgcg atcctcaaag ataagaaaac gatgcttttt acctaaggcc
     4501 accagttttt ctaccatatc ctggtcgaaa tcctcgacaa cgtcaatatg tgtcttgata
     4561 acacagacat agggtccaat tttatctacc aattctaaga tttcggattt cttcgtcaaa
     4621 tcgaccgcga ctgacaagtt gctttgcttt tcttccatca aagccaacaa ttccttggca
     4681 atgggatttt tcatcccctc agctctagct gaatagcttt gaaatactct agcatccata
     4741 actttgcttt taaaccttta atttcgatcc aagcaaaaaa gaggttcttg gtaggacaat
     4801 acggtaagaa aacacgacat gtgcagagat gccgacgaag catagttaaa ctgggatggt
     4861 aaaatcaatt aagaatttat aaagacaaaa ttgtataagt ctctaaaaca tcttaattat
     4921 acctcacaga actatctaaa atatattcac aaagtgcaaa cattatcatg aaaaagaacc
     4981 attttaattt aaagcaaggg cattaaggct tatttacaga atttcttact tttgtaaaga
     5041 ttataaggct gattatcttt ttcaccatgc caaaaattac acaagataga atggatgttt
     5101 gaaattaaac gtgagtatac aaacaaatac actaggtaaa tcgaaacatt tttttctcca
     5161 ttaagtaaca aattcctatt tagagaaaga atgctgagta gattaaataa tctatacaaa
     5221 cttttttaac acaaatgcat acatatagcc agtgggattt gtagctaagc ttgcgaattc
     5281 gagtctaact ccttaaccac tcggacatag tgacttatct gacactcatt gtaaattaat
     5341 atatatatag gcattttgtt tagttaaagg tacttaagta attagtataa acgaaccaat
     5401 tttataatca ggaagttaag tgaatggtag cacatgtcgt aaaaattgtg aatttttatt
     5461 gaataatatt ttaaatacaa gcctttctag actaggtata ctcataaaca tatatgagca
     5521 aaaggataga ggagattaca ttgcatcttc tacaaattta tttattgccc tttactgaaa
     5581 aattaaataa tgagtactaa atgataaaaa gcgctcagta caagaaagat tgcaaaaata
     5641 ttgcattctt catgaattaa agttgcatat aaggcatatt gaaagtaata gtactaaaac
     5701 agcagttagc gaaaattaat agaattatat tcgcaagaca attgtgacat taaattaaaa
     5761 aattgtagaa tttttactat cctctttaac gccatgagcc tttataaaaa ggttaaatta
     5821 gttttaacat tctttttttg agtaagagtt tacaatttat caaaacctgt gttattatat
     5881 tcattagttt caatttatta gcatctagag aaaaatcaat tggcagttac attgttggaa
     5941 tttatgaaga aaaagaatct acaacggaga ataagttgct gatcgctttc cctaaaattg
     6001 tatattttgc tgagcttatt ttgacatttc gttgaagttt tctctaattc gcattcattt
     6061 taagtaaaac aatgagaaat aaaattacaa aaaatacaaa ttaaaataca atttttagct
     6121 ataattatag acgatgccct tgtatcccat tctgtctcgc ttgcccctac tttttatctt
     6181 ttatatacca taatgaacgc tgccgctact aaccataccc cgattttaca tttcggactc
     6241 ccaaggacgt acaaaataga aaactataga aaaaaataat cagaaaatag catgtcatct
     6301 ctttgtaaaa cgcgtttgca agaagaaagg aaacaatgga gaagagatca tccatttgta
     6361 tgtaaatttt agtaaacttg aagaaatcac taacaacttc tcttacttag ggattctatg
     6421 caaaaccttg taaatcatct gatggaggac tcgatttaat gaattggaag gttgatacta
     6481 acgccgccat ccataaattg ttcatgaacg ttcttaatgt ctatttatat tatttaaaaa
     6541 gaaggagatt acgtctaaaa cgagttctcg tttaatatat gacttttaat gaaatatcta
     6601 tgttcatatt cgctacttaa ccttccttgg caagtaatcc atcctattgt taacattaat
     6661 ataatccatc aagatacttt tttattaatt taagtttttc agaattagat gttggtgcac
     6721 ctcctccaaa tctagagctg tggaaagaat tggtaaaatc ttatcgcgat gttcgtgatt
     6781 ctctaaaaat tgcagtaaaa catttctagc atattctttg ttaatcaatt ctttagaaat
     6841 attgcttata acaggtggct cgaatgaacc tgctgaatca cttgaagctt tgggcaatat
     6901 ttcactgctc ataacactgg acagctcgct ctcgtgagat ttgacacttt ccttttgaac
     6961 cttttgcttt tcaatgagtt cttttatttc atcccttgac tccactaact cgtttccaac
     7021 accgagcggc cgcatcgtga ctgactgacg atctgcctcg cgcgtttcgg tgatgacggt
     7081 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     7141 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     7201 atgacccagt cacgtagcga tagcggagtg tataattctt gaagacgaaa gggcctcgtg
     7261 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7321 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7381 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7441 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7501 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7561 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7621 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7681 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7741 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7801 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7861 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7921 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7981 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8041 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8101 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8161 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8221 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8281 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8341 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8401 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8461 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8521 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8581 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     8641 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8701 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8761 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8821 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8881 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8941 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9001 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9061 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9121 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9181 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9241 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     9301 aattccgaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa
     9361 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
     9421 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
     9481 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
     9541 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
     9601 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
     9661 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
     9721 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
     9781 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
     9841 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
     9901 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
     9961 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
    10021 atagcgg
//
