LOCUS       sequence-190560-        8221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ish1-mCherry insertion cassette with NAT resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova
            L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost A
  TITLE     LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in 
            fission yeast and human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 
            10.1073/pnas.1613916114. Epub 2017 Feb 27.
  PUBMED    28242692
REFERENCE   2  (bases 1 to 8221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1613916114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-03-14- 14"; volume: "114"; issue: "11"; pages: 
            "E2166-E2175"
COMMENT     Sequence Label: pMGF170
FEATURES             Location/Qualifiers
     source          1..8221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(22..27,28..45,46..52)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    60..76
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     84..100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     84..100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             96..623
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(116..133)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(116..132)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(125..147)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(725..730,731..746,747..753)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    761..777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             800..1453
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1411..1433
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1460..1483
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2078..2788
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2223..2241)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2491..2510
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      2809..2996
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2864..2888)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            3071..4190
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        3071..3414
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             3415..3987
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      3993..4190
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(4813..4835)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4980..4998)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5066..5170
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5171..5239,5240..6031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5389..5408)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6202..6790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6691..6710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6944..6961
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        7034..7111
                     /gene="lacI"
                     /label=lacI promoter
     CDS             7112..8194
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7131..7150)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
       61 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg
      121 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      181 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      241 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
      301 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
      361 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta
      421 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
      481 atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttggaa
      541 ttagcttatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
      601 tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
      661 gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
      721 gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
      781 cacacaggaa acagtattca tgtcccctat actaggttat tggaaaatta agggccttgt
      841 gcaacccact cgacttcttt tggaatatct tgaagaaaaa tatgaagagc atttgtatga
      901 gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa
      961 tcttccttat tatattgatg gtgatgttaa attaacacag tctatggcca tcatacgtta
     1021 tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat
     1081 gcttgaagga gcggttttgg atattagata cggtgtttcg agaattgcat atagtaaaga
     1141 ctttgaaact ctcaaagttg attttcttag caagctacct gaaatgctga aaatgttcga
     1201 agatcgttta tgtcataaaa catatttaaa tggtgatcat gtaacccatc ctgacttcat
     1261 gttgtatgac gctcttgatg ttgttttata catggaccca atgtgcctgg atgcgttccc
     1321 aaaattagtt tgttttaaaa aacgtattga agctatccca caaattgata agtacttgaa
     1381 atccagcaag tatatagcat ggcctttgca gggctggcaa gccacgtttg gtggtggcga
     1441 ccatcctcca aaatcggatc tggaagttct gttccagggg cccctggctg ttcctaattg
     1501 ggctgcttat tttgatactt tgagctccaa agagctttct gcctggatta aggaatacaa
     1561 aaaacattac catggaaaac ttcacccatc caaagatcgt gaacatcttt tccaaaatgc
     1621 ctgtaatatt tatcgcgcta ttgctgaaaa ggctgacaaa tctgttcttc aatcgattca
     1681 atcaaagttt cctaaggcaa ctgttcccgg ttatgactct tggtctaatg aagatctaaa
     1741 gtctgcactc aaggaatatg gtgacagtat tggtaaggtg tttaatcgta aagatgctat
     1801 tgaacgctta aagcgtcatg atattttatt ctatggtcct gttgttgccg acaaggctaa
     1861 atcaggagtc tctggcttcc ttcgacgtgt tgcttctttt atgggatttg gcactcgcga
     1921 cgtagctgaa attaagctag gagaaaacat tcaaaaggta aaagatactg cctctaaagc
     1981 ctcatctgct gtatcttcag caggcgacta tgtcgaaact aatgtaaaga aagcccaaag
     2041 ggttttggga ggaacgggcg gtagtggagg cactggtatg gtgagcaagg gcgaggagga
     2101 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     2161 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     2221 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     2281 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     2341 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     2401 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     2461 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     2521 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     2581 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     2641 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     2701 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     2761 cggcggcatg gacgagctgt acaagtgagg cgcgccactt ctaaataagc gaatttctta
     2821 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
     2881 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
     2941 aggttgcttt ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga
     3001 tccgctaggg ataacagggt aatatagatc tgtttagctt gcctcgtccc cgccgggtca
     3061 cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc
     3121 aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt
     3181 tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca
     3241 gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc
     3301 cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc
     3361 tttttaaatc ttgctaggat acagttctca catcacatcc gaacataaac aaccatgggt
     3421 accactcttg acgacacggc ttaccggtac cgcaccagtg tcccggggga cgccgaggcc
     3481 atcgaggcac tggatgggtc cttcaccacc gacaccgtct tccgcgtcac cgccaccggg
     3541 gacggcttca ccctgcggga ggtgccggtg gacccgcccc tgaccaaggt gttccccgac
     3601 gacgaatcgg acgacgaatc ggacgacggg gaggacggcg acccggactc tcggacgttc
     3661 gtcgcgtacg gggacgacgg cgacctggcg ggcttcgtgg tcgtctcgta ctccggctgg
     3721 aaccgccggc tgaccgtcga ggacatcgag gtcgccccgg agcaccgggg gcacggggtc
     3781 gggcgcgcgt tgatggggct cgcgacggag ttcgcccgcg agcggggtgc cgggcacctc
     3841 tggctggagg tcaccaacgt caacgcaccg gcgatccacg cgtaccggcg gatggggttc
     3901 accctctgcg gcctggacac cgccctgtac gacggcaccg cctcggacgg cgagcaggcg
     3961 ctctacatga gcatgccctg cccctaatca gtactgacaa taaaaagatt cttgttttca
     4021 agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta
     4081 gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc
     4141 agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc
     4201 gatactaacg ccgccatcca taaattgttc atgaacgttc ttaatgtcta tttatattat
     4261 ttaaaaagaa ggagattacg tctaaaacga gttctcgttt aatatatgac ttttaatgaa
     4321 atatctatgt tcatattcgc tacttaacct tccttggcaa gtaatccatc ctattgttaa
     4381 cattaatata atccatcaag atactttttt attaatttaa gtttttcaga attagatgtt
     4441 ggtgcacctc ctccaaatct agagctgtgg aaagaattgg taaaatctta tcgcgatgtt
     4501 cgtgattctc taaaaattgc agtaaaacat ttctagcata ttctttgtta atcaattctt
     4561 tagaaatatt gcttataaca ggtggctcga atgaacctgc tgaatcactt gaagctttgg
     4621 gcaatatttc actgctcata acactggaca gctcgctctc gtgagatttg acactttcct
     4681 tttgaacctt ttgcttttca atgagttctt ttatttcatc ccttgactcc actaactcgt
     4741 ttccaacacc gagcggccgc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga
     4801 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     4861 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     4921 cgcagccatg acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg
     4981 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     5041 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5101 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5161 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5221 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5281 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5341 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5401 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5461 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5521 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5581 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5641 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5701 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5761 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5821 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5881 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5941 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6001 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6061 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6121 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6181 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6241 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6301 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6361 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6421 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6481 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6541 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6601 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6661 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6721 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6781 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6841 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6901 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6961 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     7021 ccgcataaat tccgacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg
     7081 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc
     7141 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
     7201 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
     7261 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
     7321 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
     7381 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
     7441 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
     7501 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt
     7561 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
     7621 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag
     7681 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat
     7741 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
     7801 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
     7861 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
     7921 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa
     7981 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     8041 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccacgct
     8101 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8161 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc
     8221 t
//
