LOCUS       sequence-190568-        7683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  S. pombe lem2 knockout cassette with hygro resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova
            L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost A
  TITLE     LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in 
            fission yeast and human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 
            10.1073/pnas.1613916114. Epub 2017 Feb 27.
  PUBMED    28242692
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1613916114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-03-14- 14"; volume: "114"; issue: "11"; pages: 
            "E2166-E2175"
COMMENT     Sequence Label: pMGF130
FEATURES             Location/Qualifiers
     source          1..7683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(545..550,551..568,569..575)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    583..599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     607..623
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     607..623
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             619..1146
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(639..656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(639..655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(648..670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(1248..1253,1254..1269,1270..1276)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1284..1300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1323..1976
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1934..1956
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1983..2006
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     gene            2613..4188
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        2613..2956
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2957..3985
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             2957..3985
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      3991..4188
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(4798..4820)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4965..4983)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5051..5155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5156..5224,5225..6016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5374..5393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6187..6775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6676..6695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6929..6946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        7019..7096
                     /gene="lacI"
                     /label=lacI promoter
     CDS             join(7097..7683,1..496)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7116..7135)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
       61 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
      121 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
      181 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
      241 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
      301 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
      361 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacg ctagcgccca atacgcaaac
      421 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
      481 ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcacccc
      541 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      601 ttcacacagg aaacagctat gaccatgatt acggattcac tggccgtcgt tttacaacgt
      661 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      721 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      781 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
      841 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
      901 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt
      961 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
     1021 ctacaggaag gccagacgcg aattattttt gatggcgttg gaattagctt atcgactgca
     1081 cggtgcacca atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg
     1141 tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt
     1201 ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg acaattaatc
     1261 atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagtat
     1321 tcatgtcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc actcgacttc
     1381 ttttggaata tcttgaagaa aaatatgaag agcatttgta tgagcgcgat gaaggtgata
     1441 aatggcgaaa caaaaagttt gaattgggtt tggagtttcc caatcttcct tattatattg
     1501 atggtgatgt taaattaaca cagtctatgg ccatcatacg ttatatagct gacaagcaca
     1561 acatgttggg tggttgtcca aaagagcgtg cagagatttc aatgcttgaa ggagcggttt
     1621 tggatattag atacggtgtt tcgagaattg catatagtaa agactttgaa actctcaaag
     1681 ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata
     1741 aaacatattt aaatggtgat catgtaaccc atcctgactt catgttgtat gacgctcttg
     1801 atgttgtttt atacatggac ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta
     1861 aaaaacgtat tgaagctatc ccacaaattg ataagtactt gaaatccagc aagtatatag
     1921 catggccttt gcagggctgg caagccacgt ttggtggtgg cgaccatcct ccaaaatcgg
     1981 atctggaagt tctgttccag gggcccctgc tcgacatcgc tggaagcaac cattgctccg
     2041 cccggtttag aattaatttt cttcttatta cgttgaaatc tacgtccaat gtctttttgc
     2101 attttttact atagtcaaaa ttcgagtaat ccctaatgat catggattct gtattcttag
     2161 tgattgatgt tggttaagga taacttcggg ggcaacactc ttacccaata tacgttattt
     2221 gggctgaggt gacacaaagc aatgagctgc gctatgcctc cttatagagc tttcttccct
     2281 taaccattat ttgccaccca ctttaactgg caaaaagaaa gaaggaaaac aaagattcgg
     2341 cacaaagata gagaaataag aaattgcttt accttcgttt agtgaagtct tttattaaat
     2401 ttgaaaaggt gtgtttgaac agtggaattc aagcaatttc ctaagtatat tatagctcat
     2461 tgctgtaata ttataaattt gtatgggatt gcaattacgt tcacgaatca gcaacttatg
     2521 ccaggtttcc tttttaatgt agataaggat tcatgtaatt aactagctta tatcagcttt
     2581 ataccatttt tgatcagttt gaattattca ccgacatgga ggcccagaat accctccttg
     2641 acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac atttagccca
     2701 tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg gcgcgaagca
     2761 aaaattacgg ctcctcgctg cggacctgcg agcagggaaa cgctcccctc acagacgcgt
     2821 tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat ttgccactga
     2881 ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct cacatcacat
     2941 ccgaacataa acaaccatgg gtaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     3001 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     3061 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     3121 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     3181 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     3241 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     3301 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     3361 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     3421 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     3481 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     3541 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     3601 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     3661 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     3721 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     3781 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     3841 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     3901 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     3961 cactcgtccg agggcaaagg aataatcagt actgacaata aaaagattct tgttttcaag
     4021 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc
     4081 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag
     4141 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga
     4201 tactaacgcc gccatccatg attctcgact gtatttctag ctttgtattt atgacatttt
     4261 tcatagctaa tacaaaagta agttaataat atatttatct gaatgcttat acatattaaa
     4321 aatgaaacat aaaattggta taatgaggac ttgtttgcaa ggttgatgaa atttaactgt
     4381 atttcttata ttaatgtgat tcttagttat accaaataaa ttattttaaa ataaaaacca
     4441 aaaccagtac catttttgat tgaatatggg catgtctaga gtaagaaatt agcggagaaa
     4501 aaataggaca tgtcacatag aaagggtatt taaaaaaaaa taaaggaatt caaaagggag
     4561 attcacccaa cattacggtt gaagattcgt agtctgtgtc ttcttcttcc tcgtcatcat
     4621 ccaaacctgg aatttgttgc cattcgttat aaaatggttg aacaattata gaagacttcg
     4681 taagccggta aggatcaaag ggaaaatagg catccatagt gtcgaaccct tcctcgagcg
     4741 gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     4801 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     4861 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     4921 gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg tgatacgcct
     4981 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5041 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5101 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5161 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5221 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5281 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5341 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     5401 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5461 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5521 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5581 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5641 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     5701 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5761 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5821 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5881 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5941 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6001 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6061 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6121 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6181 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6241 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6301 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6361 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6421 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6481 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6541 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6601 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6661 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6721 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6781 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6841 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6901 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6961 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca taaattccga
     7021 caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
     7081 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
     7141 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
     7201 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
     7261 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
     7321 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
     7381 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
     7441 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
     7501 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
     7561 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
     7621 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
     7681 tct
//
