LOCUS       sequence-190603-        3415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal 
            promoter in a 5' entry vector for gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Tamplin OJ, Durand EM, Carr LA, Childs SJ, Hagedorn EJ, Li P, 
            Yzaguirre AD, Speck NA, Zon LI
  TITLE     Hematopoietic stem cell arrival triggers dynamic remodeling of the 
            perivascular niche.
  JOURNAL   Cell. 2015 Jan 15;160(1-2):241-52. doi: 10.1016/j.cell.2014.12.032.
  PUBMED    25594182
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2015-01-15"; volume: "160(1-2):241-52. doi"; pages: " 
            10.1016/j.cell.2014.12.032"
COMMENT     Sequence Label: p5E-Runx1+23
FEATURES             Location/Qualifiers
     source          1..3415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     88..105
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(264..291)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(383..469)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     518..540
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     532..549
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     533..549
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    589..684
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        686..704
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1438..1456)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    1457..1580
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1653..1671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1676..1692)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1676..1692)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1794..1813)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1805..2614
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1872..1891)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2761..3349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3250..3269
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
       61 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      121 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      181 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      241 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      301 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      361 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
      421 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
      481 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
      541 acggccagtc ttaagctcgg gcccgagtta acgctaccat ggagctccaa ataatgattt
      601 tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc ttttttataa
      661 tgccaacttt gtatagaaaa gttggtaata cgactcacta tagggcgaat tgggtaccgg
      721 gccccccctc gagggatccg gggtgggagg tgtaagttcc acccccaccc ttcctgacac
      781 gctcctgaac cctggccact gcacctgcta ggttctcact tctctgggaa gcatctagaa
      841 acaggacctc tcacccaccc ctcccggctg ggctctaggg tggggccctc actacctctt
      901 ttcttctcaa agagcctggg atgctgacag cctcagatgg aggcatcctg tttgtcgaaa
      961 aataaaccgg cagttgaagc cgggttgcaa gagcgagaaa accgcaggcc tgcgcgccac
     1021 tgataacgtg ggcagcttgc ttttgcagca gttcctagct gcagcggccc tgtgaaggcc
     1081 tgtgtcaccg cctcccttcc tgtctcctcc tcacaccatc cctccatcgc tccttgctgg
     1141 ctctaccagc cacttgctgg acccttcagc cactgggacc attgctttcc ataaaaaact
     1201 ccttagtgca gtgcagagct gatcagaggg tagcagggaa ccctgacgcc tgatgggttg
     1261 ctgacacctg agatccacta gtccaatctg ctcagagagg acagagtggg caggagccag
     1321 cattgggtat ataaagctga gcagggtcag ttgcttctta cgtttgcttc tgagtctgtt
     1381 gtgttgactt gcaacctcag aaacagacat cccgcggtgg agctccagct tttgttccct
     1441 ttagtgaggg ttaattcaag tttgtacaaa aaagttgaac gagaaacgta aaatgatata
     1501 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
     1561 caacatatgc agtcactatg aaccaactac ttagatggta ttagtgacct gtagaattcg
     1621 agctctagag ctgcagggcg gccgcgatat cccctatagt gagtcgtatt acatggtcat
     1681 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     1741 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     1801 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     1861 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     1921 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     1981 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     2041 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2101 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     2161 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     2221 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     2281 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     2341 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     2401 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     2461 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     2521 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     2581 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     2641 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     2701 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2761 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2821 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2881 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2941 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3001 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3061 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3121 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tcttt
//
