LOCUS       sequence-190612-        9620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct for S protein-Flag-Streptavidin 
            binding peptide (SFB)-tagged USP7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   An L, Jiang Y, Ng HH, Man EP, Chen J, Khoo US, Gong Q, Huen MS
  TITLE     Dual-utility NLS drives RNF169-dependent DNA damage responses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):E2872-E2881. doi: 
            10.1073/pnas.1616602114. Epub 2017 Mar 21.
  PUBMED    28325877
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1616602114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-04-4- 4"; volume: "114"; issue: "14"; pages: 
            "E2872-E2881"
COMMENT     Sequence Label: pMH-SFB-USP7
FEATURES             Location/Qualifiers
     source          1..9620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..310
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        311..514
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     465..485
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             574..618
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             652..675
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             679..702
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             712..825
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    898..922
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4235..4259)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4442..4459)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(4521..4541)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4526..4855
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4706..4841
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4865..5464
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4865..4884)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5361..5381
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5685..6154
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6284..6301)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6455..7043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6535..6554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7203..7994,7995..8063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7826..7845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8064..8168)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             8199..8668
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    8733..8932
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             9133..9549
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     9479..9501
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     9517..9533
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
       61 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      121 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      181 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      241 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      301 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      361 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      421 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      481 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      541 gcgctaccgg actcagatct cgaggccacc atgaaagaaa ccgctgctgc taaattcgaa
      601 cgccagcaca tggacagcgg agccggcgca ggagccggcg ctgacgcgcc tgactacaaa
      661 gacgatgacg acaagggaga ttacaaggat gacgatgaca aaggcgcgtc gatggacgag
      721 aagaccaccg gctggcgggg cggccacgtg gtggagggcc tggccggcga gctggagcag
      781 ctgcgggcca ggctggagca ccaccctcag ggccagcggg agccctccgg cggctgcaag
      841 ctgggcggcg cgcctgaatt ctgcagtcga cggtaccgcg ggcccatcgc tcgagagaca
      901 agtttgtaca aaaaagcagg cttcatgaac caccagcagc agcagcagca gcagaaagcg
      961 ggcgagcagc agttgagcga gcccgaggac atggagatgg aagcgggaga tacagatgac
     1021 ccaccaagaa ttactcagaa ccctgtgatc aatgggaatg tggccctgag tgatggacac
     1081 aacaccgcgg aggaggacat ggaggatgac accagttggc gctccgaggc aacctttcag
     1141 ttcactgtgg agcgcttcag cagactgagt gagtcggtcc ttagccctcc gtgttttgtg
     1201 cgaaatctgc catggaagat tatggtgatg ccacgctttt atccagacag accacaccaa
     1261 aaaagcgtag gattctttct ccagtgcaat gctgaatctg attccacgtc atggtcttgc
     1321 catgcacaag cagtgctgaa gataataaat tacagagatg atgaaaagtc gttcagtcgt
     1381 cgtattagtc atttgttctt ccataaagaa aatgattggg gattttccaa ttttatggcc
     1441 tggagtgaag tgaccgatcc tgagaaagga tttatagatg atgacaaagt tacctttgaa
     1501 gtctttgtac aggcggatgc tccccatgga gttgcgtggg attcaaagaa gcacacaggc
     1561 tacgtcggct taaagaatca gggagcgact tgttacatga acagcctgct acagacgtta
     1621 tttttcacga atcagctacg aaaggctgtg tacatgatgc caaccgaggg ggatgattcg
     1681 tctaaaagcg tccctttagc attacaaaga gtgttctatg aattacagca tagtgataaa
     1741 cctgtaggaa caaaaaagtt aacaaagtca tttgggtggg aaactttaga tagcttcatg
     1801 caacatgatg ttcaggagct ttgtcgagtg ttgctcgata atgtggaaaa taagatgaaa
     1861 ggcacctgtg tagagggcac catacccaaa ttattccgcg gcaaaatggt gtcctatatc
     1921 cagtgtaaag aagtagacta tcggtctgat agaagagaag attattatga tatccagcta
     1981 agtatcaaag gaaagaaaaa tatatttgaa tcatttgtgg attatgtggc agtagaacag
     2041 ctcgatgggg acaataaata cgacgctggg gaacatggct tacaggaagc agagaaaggt
     2101 gtgaaattcc taacattgcc accagtgtta catctacaac tgatgagatt tatgtatgac
     2161 cctcagacgg accaaaatat caagatcaat gataggtttg aattcccaga gcagttacca
     2221 cttgatgaat ttttgcaaaa aacagatcct aaggaccctg caaattatat tcttcatgca
     2281 gtcctggttc atagtggaga taatcatggt ggacattatg tggtttatct aaaccccaaa
     2341 ggggatggca aatggtgtaa atttgatgac gacgtggtgt caaggtgtac taaagaggaa
     2401 gcaattgagc acaattatgg gggtcacgat gacgacctgt ctgttcgaca ctgcactaat
     2461 gcttacatgt tagtctacat cagggaatca aaactgagtg aagttttaca ggcggtcacc
     2521 gaccatgata ttcctcagca gttggtggag cgattacaag aagagaaaag gatcgaggct
     2581 cagaagcgga aggagcggca ggaagcccat ctctatatgc aagtgcagat agtcgcagag
     2641 gaccagtttt gtggccacca agggaatgac atgtacgatg aagaaaaagt gaaatacact
     2701 gtgttcaaag tattgaagaa ctcctcgctt gctgagtttg ttcagagcct ctctcagacc
     2761 atgggatttc cacaagatca aattcgattg tggcccatgc aagcaaggag taatggaaca
     2821 aaacgaccag caatgttaga taatgaagcc gacggcaata aaacaatgat tgagctcagt
     2881 gataatgaaa acccttggac aatattcctg gaaacagttg atcccgagct ggctgctagt
     2941 ggagcgacct tacccaagtt tgataaagat catgatgtaa tgttattttt gaagatgtat
     3001 gatcccaaaa cgcggagctt gaattactgt gggcatatct acacaccaat atcctgtaaa
     3061 atacgtgact tgctcccagt tatgtgtgac agagcaggat ttattcaaga tactagcctt
     3121 atcctctatg aggaagttaa accgaattta acagagagaa ttcaggacta tgacgtgtct
     3181 cttgataaag cccttgatga actaatggat ggtgacatca tagtatttca gaaggatgac
     3241 cctgaaaatg ataacagtga attacccacc gcaaaggagt atttccgaga tctctaccac
     3301 cgcgttgatg tcattttctg tgataaaaca atccctaatg atcctggatt tgtggttacg
     3361 ttatcaaata gaatgaatta ttttcaggtt gcaaagacag ttgcacagag gctcaacaca
     3421 gatccaatgt tgctgcagtt tttcaagtct caaggttata gggatggccc aggtaatcct
     3481 cttagacata attatgaagg tactttaaga gatcttctac agttcttcaa gcctagacaa
     3541 cctaagaaac tttactatca gcagcttaag atgaaaatca cagactttga gaacaggcga
     3601 agttttaaat gtatatggtt aaacagccaa tttagggaag aggaaataac actatatcca
     3661 gacaagcatg ggtgtgtccg ggacctgtta gaagaatgta aaaaggccgt ggagcttggg
     3721 gagaaagcat cagggaaact taggctgcta gaaattgtaa gctacaaaat cattggtgtt
     3781 catcaagaag atgaactatt agaatgttta tctcctgcaa cgagccggac gtttcgaata
     3841 gaggaaatcc ctttggacca ggtggacata gacaaagaga atgagatgct tgtcacagtg
     3901 gcgcatttcc acaaagaggt cttcggaacg ttcggaatcc cgtttttgct gaggatacac
     3961 cagggcgagc attttcgaga agtgatgaag cgaatccaga gcctgctgga catccaggag
     4021 aaggagtttg agaagtttaa atttgcaatt gtaatgatgg gccgacacca gtacataaat
     4081 gaagacgagt atgaagtaaa tttgaaagac tttgagccac agcccggtaa tatgtctcat
     4141 cctcggcctt ggctagggct cgaccacttc aacaaagccc caaagaggag tcgctacact
     4201 taccttgaaa aggccattaa aatccataac tgatacccag ctttcttgta caaagtggct
     4261 cgagcaggga tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     4321 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     4381 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     4441 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcgta ggaattcgcc
     4501 agcacagtgg tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4561 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4621 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4681 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4741 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4801 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4861 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     4921 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     4981 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     5041 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     5101 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     5161 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     5221 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     5281 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     5341 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     5401 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     5461 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     5521 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     5581 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
     5641 gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa
     5701 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc
     5761 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     5821 aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt
     5881 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     5941 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     6001 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
     6061 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     6121 gagtgattga ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt
     6181 ggtttttttt cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac
     6241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6961 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7141 cctagatcct tttgcggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac
     7201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7501 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7801 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8161 gttccgcgca catttccccg aaaagtgcca cctgcagcct gaatatgggc caaacaggat
     8221 atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata
     8281 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     8341 tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg
     8401 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
     8461 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
     8521 tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc
     8581 tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga
     8641 ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct
     8701 gcccagggac caccgaccca ccaccgggag gcaagctggc cagcaactta tctgtgtctg
     8761 tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac
     8821 tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc cggccgcaac
     8881 cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg aggaagggag
     8941 tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga gaacctaaaa
     9001 cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg cgcgtcttgt
     9061 ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg
     9121 tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa
     9181 gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc
     9241 ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac
     9301 cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca
     9361 gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc
     9421 aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc
     9481 cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct
     9541 ctaggcgccg gccggatccc agtgtggtgg tactaatagt aatcaattac ggggtcatta
     9601 gttcatagcc catatatgga
//
