LOCUS       sequence-190615-       11902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct for S protein-Flag-Streptavidin 
            binding peptide (SFB)-tagged BRCA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Sy SM, Huen MS, Chen J
  TITLE     PALB2 is an integral component of the BRCA complex required for 
            homologous recombination repair.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7155-60. doi: 
            10.1073/pnas.0811159106. Epub 2009 Apr 15.
  PUBMED    19369211
REFERENCE   2  (bases 1 to 11902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0811159106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-04-28- 28"; volume: "106"; issue: "17"; pages: "7155-60"
COMMENT     Sequence Label: pMH-SFB-BRCA1
FEATURES             Location/Qualifiers
     source          1..11902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        43..346
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        347..550
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     501..521
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             610..654
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             688..711
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             715..738
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             748..861
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    934..958
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6553..6577)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6760..6777)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(6839..6859)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6844..7173
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7024..7159
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7183..7782
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7183..7202)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7679..7699
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8003..8472
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8602..8619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8773..9361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8853..8872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9521..10312,10313..10381))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10144..10163
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10382..10486)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             10517..10986
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    11051..11250
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             11451..11867
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     11797..11819
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     11835..11851
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
       61 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      121 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      181 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      241 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      301 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      361 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      421 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      481 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      541 aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag
      601 gccaccatga aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga cagcggagcc
      661 ggcgcaggag ccggcgctga cgcgcctgac tacaaagacg atgacgacaa gggagattac
      721 aaggatgacg atgacaaagg cgcgtcgatg gacgagaaga ccaccggctg gcggggcggc
      781 cacgtggtgg agggcctggc cggcgagctg gagcagctgc gggccaggct ggagcaccac
      841 cctcagggcc agcgggagcc ctccggcggc tgcaagctgg gcggcgcgcc tgaattctgc
      901 agtcgacggt accgcgggcc catcgctcga gagacaagtt tgtacaaaaa agcaggctcc
      961 atggatttat ctgctcttcg cgttgaagaa gtacaaaatg tcattaatgc tatgcagaaa
     1021 atcttagagt gtcccatctg tctggagttg atcaaggaac ctgtctccac aaagtgtgac
     1081 cacatatttt gcaaattttg catgctgaaa cttctcaacc agaagaaagg gccttcacag
     1141 tgtcctttat gtaagaatga tataaccaaa aggagcctac aagaaagtac gagatttagt
     1201 caacttgttg aagagctatt gaaaatcatt tgtgcttttc agcttgacac aggtttggag
     1261 tatgcaaaca gctataattt tgcaaaaaag gaaaataact ctcctgaaca tctaaaagat
     1321 gaagtttcta tcatccaaag tatgggctac agaaaccgtg ccaaaagact tctacagagt
     1381 gaacccgaaa atccttcctt gcaggaaacc agtctcagtg tccaactctc taaccttgga
     1441 actgtgagaa ctctgaggac aaagcagcgg atacaacctc aaaagacgtc tgtctacatt
     1501 gaattgggat ctgattcttc tgaagatacc gttaataagg caacttattg cagtgtggga
     1561 gatcaagaat tgttacaaat cacccctcaa ggaaccaggg atgaaatcag tttggattct
     1621 gcaaaaaagg ctgcttgtga attttctgag acggatgtaa caaatactga acatcatcaa
     1681 cccagtaata atgatttgaa caccactgag aagcgtgcag ctgagaggca tccagaaaag
     1741 tatcagggta gttctgtttc aaacttgcat gtggagccat gtggcacaaa tactcatgcc
     1801 agctcattac agcatgagaa cagcagttta ttactcacta aagacagaat gaatgtagaa
     1861 aaggctgaat tctgtaataa aagcaaacag cctggcttag caaggagcca acataacaga
     1921 tgggctggaa gtaaggaaac atgtaatgat aggcggactc ccagcacaga aaaaaaggta
     1981 gatctgaatg ctgatcccct gtgtgagaga aaagaatgga ataagcagaa actgccatgc
     2041 tcagagaatc ctagagatac tgaagatgtt ccttggataa cactaaatag cagcattcag
     2101 aaagttaatg agtggttttc cagaagtgat gaactgttag gttctgatga ctcacatgat
     2161 ggggagtctg aatcaaatgc caaagtagct gatgtattgg acgttctaaa tgaggtagat
     2221 gaatattctg gttcttcaga gaaaatagac ttactggcca gtgatcctca tgaggcttta
     2281 atatgtaaaa gtgaaagagt tcactccaaa tcagtagaga gtaatattga agacaaaata
     2341 tttgggaaaa cctatcggaa gaaggcaagc ctccccaact taagccatgt aactgaaaat
     2401 ctaattatag gagcatttgt tactgagcca cagataatac aagagcgtcc cctcacaaat
     2461 aaattaaagc gtaaaaggag acctacatca ggccttcatc ctgaggattt tatcaagaaa
     2521 gcagatttgg cagttcaaaa gactcctgaa atgataaatc agggaactaa ccaaacggag
     2581 cagaatggtc aagtgatgaa tattactaat agtggtcatg agaataaaac aaaaggtgat
     2641 tctattcaga atgagaaaaa tcctaaccca atagaatcac tcgaaaaaga atctgctttc
     2701 aaaacgaaag ctgaacctat aagcagcagt ataagcaata tggaactcga attaaatatc
     2761 cacaattcaa aagcacctaa aaagaatagg ctgaggagga agtcttctac caggcatatt
     2821 catgcgcttg aactagtagt cagtagaaat ctaagcccac ctaattgtac tgaattgcaa
     2881 attgatagtt gttctagcag tgaagagata aagaaaaaaa agtacaacca aatgccagtc
     2941 aggcacagca gaaacctaca actcatggaa ggtaaagaac ctgcaactgg agccaagaag
     3001 agtaacaagc caaatgaaca gacaagtaaa agacatgaca gtgatacttt cccagagctg
     3061 aagttaacaa atgcacctgg ttcttttact aagtgttcaa ataccagtga acttaaagaa
     3121 tttgtcaatc ctagccttcc aagagaagaa aaagaagaga aactagaaac agttaaagtg
     3181 tctaataatg ctgaagaccc caaagatctc atgttaagtg gagaaagggt tttgcaaact
     3241 gaaagatctg tagagagtag cagtatttca ctggtacctg gtactgatta tggcactcag
     3301 gaaagtatct cgttactgga agttagcact ctagggaagg caaaaacaga accaaataaa
     3361 tgtgtgagtc agtgtgcagc atttgaaaac cccaagggac taattcatgg ttgttccaaa
     3421 gataatagaa atgacacaga aggctttaag tatccattgg gacatgaagt taaccacagt
     3481 cgggaaacaa gcatagaaat ggaagaaagt gaacttgatg ctcagtattt gcagaataca
     3541 ttcaaggttt caaagcgcca gtcatttgct ctgttttcaa atccaggaaa tgcagaagag
     3601 gaatgtgcaa cattctctgc ccactctggg tccttaaaga aacaaagtcc aaaagtcact
     3661 tttgaatgtg aacaaaagga agaaaatcaa ggaaagaatg agtctaatat caagcctgta
     3721 cagacagtta atatcactgc aggctttcct gtggttggtc agaaagataa gccagttgat
     3781 aatgccaaat gtagtatcaa aggaggctct aggttttgtc tatcatctca gttcagaggc
     3841 aacgaaactg gactcattac tccaaataaa catggacttt tacaaaaccc atatcgtata
     3901 ccaccacttt ttcccatcaa gtcatttgtt aaaactaaat gtaagaaaaa tctgctagag
     3961 gaaaactttg aggaacattc aatgtcacct gaaagagaaa tgggaaatga gaacattcca
     4021 agtacagtga gcacaattag ccgtaataac attagagaaa atgtttttaa aggagccagc
     4081 tcaagcaata ttaatgaagt aggttccagt actaatgaag tgggctccag tattaatgaa
     4141 ataggttcca gtgatgaaaa cattcaagca gaactaggta gaaacagagg gccaaaattg
     4201 aatgctatgc ttagattagg ggttttgcaa cctgaggtct ataaacaaag tcttcctgga
     4261 agtaattgta agcatcctga aataaaaaag caagaatatg aagaagtagt tcagactgtt
     4321 aatacagatt tctctccata tctgatttca gataacttag aacagcctat gggaagtagt
     4381 catgcatctc aggtttgttc tgagacacct gatgacctgt tagatgatgg tgaaataaag
     4441 gaagatacta gttttgctga aaatgacatt aaggaaagtt ctgctgtttt tagcaaaagc
     4501 gtccagagag gagagcttag caggagtcct agccctttca cccatacaca tttggctcag
     4561 ggttaccgaa gaggggccaa gaaattagag tcctcagaag agaacttatc tagtgaggat
     4621 gaagagcttc cctgcttcca acacttgtta tttggtaaag taaacaatat accttctcag
     4681 tctactaggc atagcaccgt tgctaccgag tgtctgtcta agaacacaga ggagaattta
     4741 ttatcattga agaatagctt aaatgactgc agtaaccagg taatattggc aaaggcatct
     4801 caggaacatc accttagtga ggaaacaaaa tgttctgcta gcttgttttc ttcacagtgc
     4861 agtgaattgg aagacttgac tgcaaataca aacacccagg atcctttctt gattggttct
     4921 tccaaacaaa tgaggcatca gtctgaaagc cagggagttg gtctgagtga caaggaattg
     4981 gtttcagatg atgaagaaag aggaacgggc ttggaagaaa ataatcaaga agagcaaagc
     5041 atggattcaa acttaggtga agcagcatct gggtgtgaga gtgaaacaag cgtctctgaa
     5101 gactgctcag ggctatcctc tcagagtgac attttaacca ctcagcagag ggataccatg
     5161 caacataacc tgataaagct ccagcaggaa atggctgaac tagaagctgt gttagaacag
     5221 catgggagcc agccttctaa cagctaccct tccatcataa gtgactcctc tgcccttgag
     5281 gacctgcgaa atccagaaca aagcacatca gaaaaagcag tattaacttc acagaaaagt
     5341 agtgaatacc ctataagcca gaatccagaa ggcctttctg ctgacaagtt tgaggtgtct
     5401 gcagatagtt ctaccagtaa aaataaagaa ccaggagtgg aaaggtcatc cccttctaaa
     5461 tgcccatcat tagatgatag gtggtacatg cacagttgct ctgggagtct tcagaataga
     5521 aactacccat ctcaagagga gctcattaag gttgttgatg tggaggagca acagctggaa
     5581 gagtctgggc cacacgattt gacggaaaca tcttacttgc caaggcaaga tctagaggga
     5641 accccttacc tggaatctgg aatcagcctc ttctctgatg accctgaatc tgatccttct
     5701 gaagacagag ccccagagtc agctcgtgtt ggcaacatac catcttcaac ctctgcattg
     5761 aaagttcccc aattgaaagt tgcagaatct gcccagggtc cagctgctgc tcatactact
     5821 gatactgctg ggtataatgc aatggaagaa agtgtgagca gggagaagcc agaattgaca
     5881 gcttcaacag aaagggtcaa caaaagaatg tccatggtgg tgtctggcct gaccccagaa
     5941 gaatttatgc tcgtgtacaa gtttgccaga aaacaccaca tcactttaac taatctaatt
     6001 actgaagaga ctactcatgt tgttatgaaa acagatgctg agtttgtgtg tgaacggaca
     6061 ctgaaatatt ttctaggaat tgcgggagga aaatgggtag ttagctattt ctgggtgacc
     6121 cagtctatta aagaaagaaa aatgctgaat gagcatgatt ttgaagtcag aggagatgtg
     6181 gtcaatggaa gaaaccacca aggtccaaag cgagcaagag aatcccagga cagaaagatc
     6241 ttcagggggc tagaaatctg ttgctatggg cccttcacca acatgcccac agatcaactg
     6301 gaatggatgg tacagctgtg tggtgcttct gtggtgaagg agctttcatc attcaccctt
     6361 ggcacaggtg tccacccaat tgtggttgtg cagccagatg cctggacaga ggacaatggc
     6421 ttccatgcaa ttgggcagat gtgtgaggca cctgtggtga cccgagagtg ggtgttggac
     6481 agtgtagcac tctaccagtg ccaggagctg gacacctacc tgatacccca gatcccccac
     6541 agccactact gaacccagct ttcttgtaca aagtggctcg agcagggatc cgcccctctc
     6601 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     6661 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     6721 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     6781 gtctgttgaa tgtcgtgaag gaagcgtagg aattcgccag cacagtggtc gaccctgtgg
     6841 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6901 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6961 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7021 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7081 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7141 ggaggctttt ttggaggcct aggcttttgc aaaaagctta ccatgaccga gtacaagccc
     7201 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     7261 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     7321 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     7381 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     7441 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     7501 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     7561 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     7621 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     7681 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     7741 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     7801 cagcgcccga ccgaaaggag cgcacgaccc catgcatcga taaaataaaa gattttattt
     7861 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     7921 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     7981 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     8041 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
     8101 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     8161 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     8221 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     8281 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     8341 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     8401 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     8461 gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta
     8521 cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8581 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8641 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8701 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9181 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccgca
     9481 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9541 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9601 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9661 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9721 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9781 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9841 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9901 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9961 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10021 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10081 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10141 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10201 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10261 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10321 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10381 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10441 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10501 aagtgccacc tgcagcctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
    10561 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
    10621 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
    10681 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
    10741 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
    10801 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
    10861 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
    10921 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
    10981 ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc
    11041 accgggaggc aagctggcca gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga
    11101 ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc tggcggaccc
    11161 gtggtggaac tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact
    11221 ttgggggccg tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt
    11281 caggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat
    11341 ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg
    11401 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg
    11461 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca
    11521 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa
    11581 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta
    11641 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga
    11701 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc
    11761 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc
    11821 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc cggatcccag
    11881 tgtggtggta ctaatagtaa tc
//
