LOCUS       sequence-190616-       16567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct for S protein-Flag-Streptavidin 
            binding peptide (SFB)-tagged BRCA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16567)
  AUTHORS   Sy SM, Huen MS, Chen J
  TITLE     PALB2 is an integral component of the BRCA complex required for 
            homologous recombination repair.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7155-60. doi: 
            10.1073/pnas.0811159106. Epub 2009 Apr 15.
  PUBMED    19369211
REFERENCE   2  (bases 1 to 16567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0811159106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-04-28- 28"; volume: "106"; issue: "17"; pages: "7155-60"
COMMENT     Sequence Label: pMH-SFB-BRCA2
FEATURES             Location/Qualifiers
     source          1..16567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..353
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        354..557
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     508..528
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             617..661
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             695..718
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             722..745
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             755..868
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    941..965
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11225..11249)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11432..11449)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(11511..11531)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11516..11845
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11696..11831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11855..12454
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11855..11874)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12351..12371
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             12675..13144
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(13274..13291)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13445..14033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13525..13544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14193..14984,14985..15053))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14816..14835
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15054..15158)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             15189..15658
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    15723..15922
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             16123..16539
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     16469..16491
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     16507..16523
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
       61 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      121 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      181 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      241 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      301 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      361 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      421 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      481 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      541 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggactcaga
      601 tctcgaggcc accatgaaag aaaccgctgc tgctaaattc gaacgccagc acatggacag
      661 cggagccggc gcaggagccg gcgctgacgc gcctgactac aaagacgatg acgacaaggg
      721 agattacaag gatgacgatg acaaaggcgc gtcgatggac gagaagacca ccggctggcg
      781 gggcggccac gtggtggagg gcctggccgg cgagctggag cagctgcggg ccaggctgga
      841 gcaccaccct cagggccagc gggagccctc cggcggctgc aagctgggcg gcgcgcctga
      901 attctgcagt cgacggtacc gcgggcccat cgctcgagag acaagtttgt acaaaaaagc
      961 aggcttcatg cctattggat ccaaagagag gccaacattt tttgaaattt ttaagacacg
     1021 ctgcaacaaa gcagatttag gaccaataag tcttaattgg tttgaagaac tttcttcaga
     1081 agctccaccc tataattctg aacctgcaga agaatctgaa cataaaaaca acaattacga
     1141 accaaaccta tttaaaactc cacaaaggaa accatcttat aatcagctgg cttcaactcc
     1201 aataatattc aaagagcaag ggctgactct gccgctgtac caatctcctg taaaagaatt
     1261 agataaattc aaattagact taggaaggaa tgttcccaat agtagacata aaagtcttcg
     1321 cacagtgaaa actaaaatgg atcaagcaga tgatgtttcc tgtccacttc taaattcttg
     1381 tcttagtgaa agtcctgttg ttctacaatg tacacatgta acaccacaaa gagataagtc
     1441 agtggtatgt gggagtttgt ttcatacacc aaagtttgtg aagggtcgtc agacaccaaa
     1501 acatatttct gaaagtctag gagctgaggt ggatcctgat atgtcttggt caagttcttt
     1561 agctacacca cccaccctta gttctactgt gctcatagtc agaaatgaag aagcatctga
     1621 aactgtattt cctcatgata ctactgctaa tgtgaaaagc tatttttcca atcatgatga
     1681 aagtctgaag aaaaatgata gatttatcgc ttctgtgaca gacagtgaaa acacaaatca
     1741 aagagaagct gcaagtcatg gatttggaaa aacatcaggg aattcattta aagtaaatag
     1801 ctgcaaagac cacattggaa agtcaatgcc aaatgtccta gaagatgaag tatatgaaac
     1861 agttgtagat acctctgaag aagatagttt ttcattatgt ttttctaaat gtagaacaaa
     1921 aaatctacaa aaagtaagaa ctagcaagac taggaaaaaa attttccatg aagcaaacgc
     1981 tgatgaatgt gaaaaatcta aaaaccaagt gaaagaaaaa tactcatttg tatctgaagt
     2041 ggaaccaaat gatactgatc cattagattc aaatgtagca catcagaagc cctttgagag
     2101 tggaagtgac aaaatctcca aggaagttgt accgtctttg gcctgtgaat ggtctcaact
     2161 aaccctttca ggtctaaatg gagcccagat ggagaaaata cccctattgc atatttcttc
     2221 atgtgaccaa aatatttcag aaaaagacct attagacaca gagaacaaaa gaaagaaaga
     2281 ttttcttact tcagagaatt ctttgccacg tatttctagc ctaccaaaat cagagaagcc
     2341 attaaatgag gaaacagtgg taaataagag agatgaagag cagcatcttg aatctcatac
     2401 agactgcatt cttgcagtaa agcaggcaat atctggaact tctccagtgg cttcttcatt
     2461 tcagggtatc aaaaagtcta tattcagaat aagagaatca cctaaagaga ctttcaatgc
     2521 aagtttttca ggtcatatga ctgatccaaa ctttaaaaaa gaaactgaag cctctgaaag
     2581 tggactggaa atacatactg tttgctcaca gaaggaggac tccttatgtc caaatttaat
     2641 tgataatgga agctggccag ccaccaccac acagaattct gtagctttga agaatgcagg
     2701 tttaatatcc actttgaaaa agaaaacaaa taagtttatt tatgctatac atgatgaaac
     2761 attttataaa ggaaaaaaaa taccgaaaga ccaaaaatca gaactaatta actgttcagc
     2821 ccagtttgaa gcaaatgctt ttgaagcacc acttacattt gcaaatgctg attcaggttt
     2881 attgcattct tctgtgaaaa gaagctgttc acagaatgat tctgaagaac caactttgtc
     2941 cttaactagc tcttttggga caattctgag gaaatgttct agaaatgaaa catgttctaa
     3001 taatacagta atctctcagg atcttgatta taaagaagca aaatgtaata aggaaaaact
     3061 acagttattt attaccccag aagctgattc tctgtcatgc ctgcaggaag gacagtgtga
     3121 aaatgatcca aaaagcaaaa aagtttcaga tataaaagaa gaggtcttgg ctgcagcatg
     3181 tcacccagta caacattcaa aagtggaata cagtgatact gactttcaat cccagaaaag
     3241 tcttttatat gatcatgaaa atgccagcac tcttatttta actcctactt ccaaggatgt
     3301 tctgtcaaac ctagtcatga tttctagagg caaagaatca tacaaaatgt cagacaagct
     3361 caaaggtaac aattatgaat ctgatgttga attaaccaaa aatattccca tggaaaagaa
     3421 tcaagatgta tgtgctttaa atgaaaatta taaaaacgtt gagctgttgc cacctgaaaa
     3481 atacatgaga gtagcatcac cttcaagaaa ggtacaattc aaccaaaaca caaatctaag
     3541 agtaatccaa aaaaatcaag aagaaactac ttcaatttca aaaataactg tcaatccaga
     3601 ctctgaagaa cttttctcag acaatgagaa taattttgtc ttccaagtag ctaatgaaag
     3661 gaataatctt gctttaggaa atactaagga acttcatgaa acagacttga cttgtgtaaa
     3721 cgaacccatt ttcaagaact ctaccatggt tttatatgga gacacaggtg ataaacaagc
     3781 aacccaagtg tcaattaaaa aagatttggt ttatgttctt gcagaggaga acaaaaatag
     3841 tgtaaagcag catataaaaa tgactctagg tcaagattta aaatcggaca tctccttgaa
     3901 tatagataaa ataccagaaa aaaataatga ttacatgaac aaatgggcag gactcttagg
     3961 tccaatttca aatcacagtt ttggaggtag cttcagaaca gcttcaaata aggaaatcaa
     4021 gctctctgaa cataacatta agaagagcaa aatgttcttc aaagatattg aagaacaata
     4081 tcctactagt ttagcttgtg ttgaaattgt aaataccttg gcattagata atcaaaagaa
     4141 actgagcaag cctcagtcaa ttaatactgt atctgcacat ttacagagta gtgtagttgt
     4201 ttctgattgt aaaaatagtc atataacccc tcagatgtta ttttccaagc aggattttaa
     4261 ttcaaaccat aatttaacac ctagccaaaa ggcagaaatt acagaacttt ctactatatt
     4321 agaagaatca ggaagtcagt ttgaatttac tcagtttaga aagccaagct acatattgca
     4381 gaagagtaca tttgaagtgc ctgaaaacca gatgactatc ttaaagacca cttctgagga
     4441 atgcagagat gctgatcttc atgtcataat gaatgcccca tcgattggtc aggtagacag
     4501 cagcaagcaa tttgaaggta cagttgaaat taaacggaag tttgctggcc tgttgaaaaa
     4561 tgactgtaac aaaagtgctt ctggttattt aacagatgaa aatgaagtgg ggtttagggg
     4621 cttttattct gctcatggca caaaactgaa tgtttctact gaagctctgc aaaaagctgt
     4681 gaaactgttt agtgatattg agaatattag tgaggaaact tctgcagagg tacatccaat
     4741 aagtttatct tcaagtaaat gtcatgattc tgttgtttca atgtttaaga tagaaaatca
     4801 taatgataaa actgtaagtg aaaaaaataa taaatgccaa ctgatattac aaaataatat
     4861 tgaaatgact actggcactt ttgttgaaga aattactgaa aattacaaga gaaatactga
     4921 aaatgaagat aacaaatata ctgctgccag tagaaattct cataacttag aatttgatgg
     4981 cagtgattca agtaaaaatg atactgtttg tattcataaa gatgaaacgg acttgctatt
     5041 tactgatcag cacaacatat gtcttaaatt atctggccag tttatgaagg agggaaacac
     5101 tcagattaaa gaagatttgt cagatttaac ttttttggaa gttgcgaaag ctcaagaagc
     5161 atgtcatggt aatacttcaa ataaagaaca gttaactgct actaaaacgg agcaaaatat
     5221 aaaagatttt gagacttctg atacattttt tcagactgca agtgggaaaa atattagtgt
     5281 cgccaaagag tcatttaata aaattgtaaa tttctttgat cagaaaccag aagaattgca
     5341 taacttttcc ttaaattctg aattacattc tgacataaga aagaacaaaa tggacattct
     5401 aagttatgag gaaacagaca tagttaaaca caaaatactg aaagaaagtg tcccagttgg
     5461 tactggaaat caactagtga ccttccaggg acaacccgaa cgtgatgaaa agatcaaaga
     5521 acctactctg ttgggttttc atacagctag cgggaaaaaa gttaaaattg caaaggaatc
     5581 tttggacaaa gtgaaaaacc tttttgatga aaaagagcaa ggtactagtg aaatcaccag
     5641 ttttagccat caatgggcaa agaccctaaa gtacagagag gcctgtaaag accttgaatt
     5701 agcatgtgag accattgaga tcacagctgc cccaaagtgt aaagaaatgc agaattctct
     5761 caataatgat aaaaaccttg tttctattga gactgtggtg ccacctaagc tcttaagtga
     5821 taatttatgt agacaaactg aaaatctcaa aacatcaaaa agtatctttt tgaaagttaa
     5881 agtacatgaa aatgtagaaa aagaaacagc aaaaagtcct gcaacttgtt acacaaatca
     5941 gtccccttat tcagtcattg aaaattcagc cttagctttt tacacaagtt gtagtagaaa
     6001 aacttctgtg agtcagactt cattacttga agcaaaaaaa tggcttagag aaggaatatt
     6061 tgatggtcaa ccagaaagaa taaatactgc agattatgta ggaaattatt tgtatgaaaa
     6121 taattcaaac agtactatag ctgaaaatga caaaaatcat ctctccgaaa aacaagatac
     6181 ttatttaagt aacagtagca tgtctaacag ctattcctac cattctgatg aggtatataa
     6241 tgattcagga tatctctcaa aaaataaact tgattctggt attgagccag tattgaagaa
     6301 tgttgaagat caaaaaaaca ctagtttttc caaagtaata tccaatgtaa aagatgcaaa
     6361 tgcataccca caaactgtaa atgaagatat ttgcgttgag gaacttgtga ctagctcttc
     6421 accctgcaaa aataaaaatg cagccattaa attgtccata tctaatagta ataattttga
     6481 ggtagggcca cctgcattta ggatagccag tggtaaaatc gtttgtgttt cacatgaaac
     6541 aattaaaaaa gtgaaagaca tatttacaga cagtttcagt aaagtaatta aggaaaacaa
     6601 cgagaataaa tcaaaaattt gccaaacgaa aattatggca ggttgttacg aggcattgga
     6661 tgattcagag gatattcttc ataactctct agataatgat gaatgtagca cgcattcaca
     6721 taaggttttt gctgacattc agagtgaaga aattttacaa cataaccaaa atatgtctgg
     6781 attggagaaa gtttctaaaa tatcaccttg tgatgttagt ttggaaactt cagatatatg
     6841 taaatgtagt atagggaagc ttcataagtc agtctcatct gcaaatactt gtgggatttt
     6901 tagcacagca agtggaaaat ctgtccaggt atcagatgct tcattacaaa acgcaagaca
     6961 agtgttttct gaaatagaag gtagtaccaa gcaagtcttt tccaaagtat tgtttaaaag
     7021 taacgaacat tcagaccagc tcacaagaga agaaaatact gctatacgta ctccagaaca
     7081 tttaatatcc caaaaaggct tttcatataa tgtggtaaat tcatctgctt tctctggatt
     7141 tagtacagca agtggaaagc aagtttccat tttagaaagt tccttacaca aagttaaggg
     7201 agtgttagag gaatttgatt taatcagaac tgagcatagt cttcactatt cacctacgtc
     7261 tagacaaaat gtatcaaaaa tacttcctcg tgttgataag agaaacccag agcactgtgt
     7321 aaactcagaa atggaaaaaa cctgcagtaa agaatttaaa ttatcaaata acttaaatgt
     7381 tgaaggtggt tcttcagaaa ataatcactc tattaaagtt tctccatatc tctctcaatt
     7441 tcaacaagac aaacaacagt tggtattagg aaccaaagtc tcacttgttg ggaacattca
     7501 tgttttggga aaagaacagg cttcacctaa aaacgtaaaa atggaaattg gtaaaactga
     7561 aactttttct gatgttcctg tgaaaacaaa tatagaagtt tgttctactt actccaaaga
     7621 ttcagaaaac tactttgaaa cagaagcagt agaaattgct aaagctttta tggaagatga
     7681 tgaactgaca gattctaaac tgccaagtca tgccacacat tctcttttta catgtcccga
     7741 aaatgaggaa atggttttgt caaattcaag aattggaaaa agaagaggag agccccttat
     7801 cttagtggga gaaccctcaa tcaaaagaaa cttattaaat gaatttgaca ggataataga
     7861 aaatcaagaa aaatccttaa aggcttcaaa aagcactcca gatggcacaa taaaagatcg
     7921 aagattgttt atgcatcatg tttctttaga gccgattacc tgtgtaccct ttcgcacaac
     7981 taaggaacgt caagagatac agaatccaaa ttttaccgca cctggtcaag aatttctgtc
     8041 taaatctcat ttgtatgaac atctgacttt ggaaaaatct tcaagcaatt tagcagtttc
     8101 aggacatcca ttttatcaag tttctgctac aagaaatgaa aaaatgagac acttgattac
     8161 tacaggcaga ccaaccaaag tctttgttcc accttttaaa actaaatcac attttcacag
     8221 agttgaacag tgtgttagga atattaactt ggaggaaaac agacaaaagc aaaacattga
     8281 tggacatggc tctgatgata gtaaaaataa gattaatgac aatgagattc atcagtttaa
     8341 caaaaacaac tccaatcaag cagcagctgt aactttcaca aagtgtgaag aagaaccttt
     8401 agatttaatt acaagtcttc agaatgccag agatatacag gatatgcgaa ttaagaagaa
     8461 acaaaggcaa cgcgtctttc cacagccagg cagtctgtat cttgcaaaaa catccactct
     8521 gcctcgaatc tctctgaaag cagcagtagg aggccaagtt ccctctgcgt gttctcataa
     8581 acagctgtat acgtatggcg tttctaaaca ttgcataaaa attaacagca aaaatgcaga
     8641 gtcttttcag tttcacactg aagattattt tggtaaggaa agtttatgga ctggaaaagg
     8701 aatacagttg gctgatggtg gatggctcat accctccaat gatggaaagg ctggaaaaga
     8761 agaattttat agggctctgt gtgacactcc aggtgtggat ccaaagctta tttctagaat
     8821 ttgggtttat aatcactata gatggatcat atggaaactg gcagctatgg aatgtgcctt
     8881 tcctaaggaa tttgctaata gatgcctaag cccagaaagg gtgcttcttc aactaaaata
     8941 cagatatgat acggaaattg atagaagcag aagatcggct ataaaaaaga taatggaaag
     9001 ggatgacaca gctgcaaaaa cacttgttct ctgtgtttct gacataattt cattgagcgc
     9061 aaatatatct gaaacttcta gcaataaaac tagtagtgca gatacccaaa aagtggccat
     9121 tattgaactt acagatgggt ggtatgctgt taaggcccag ttagatcctc ccctcttagc
     9181 tgtcttaaag aatggcagac tgacagttgg tcagaagatt attcttcatg gagcagaact
     9241 ggtgggctct cctgatgcct gtacacctct tgaagcccca gaatctctta tgttaaagat
     9301 ttctgctaac agtactcggc ctgctcgctg gtataccaaa cttggattct ttcctgaccc
     9361 tagacctttt cctctgccct tatcatcgct tttcagtgat ggaggaaatg ttggttgtgt
     9421 tgatgtaatt attcaaagag cataccctat acagtggatg gagaagacat catctggatt
     9481 atacatattt cgcaatgaaa gagaggaaga aaaggaagca gcaaaatatg tggaggccca
     9541 acaaaagaga ctagaagcct tattcactaa aattcaggag gaatttgaag aacatgaaga
     9601 aaacacaaca aaaccatatt taccatcacg tgcactaaca agacagcaag ttcgtgcttt
     9661 gcaagatggt gcagagcttt atgaagcagt gaagaatgca gcagacccag cttaccttga
     9721 gggttatttc agtgaagagc agttaagagc cttgaataat cacaggcaaa tgttgaatga
     9781 taagaaacaa gctcagatcc agttggaaat taggaaggcc atggaatctg ctgaacaaaa
     9841 ggaacaaggt ttatcaaggg atgtcacaac cgtgtggaag ttgcgtattg taagctattc
     9901 aaaaaaagaa aaagattcag ttatactgag tatttggcgt ccatcatcag atttatattc
     9961 tctgttaaca gaaggaaaga gatacagaat ttatcatctt gcaacttcaa aatctaaaag
    10021 taaatctgaa agagctaaca tacagttagc agcgacaaaa aaaactcagt atcaacaact
    10081 accggtttca gatgaaattt tatttcagat ttaccagcca cgggagcccc ttcacttcag
    10141 caaattttta gatccagact ttcagccatc ttgttctgag gtggacctaa taggatttgt
    10201 cgtttctgtt gtgaaaaaaa caggacttgc ccctttcgtc tatttgtcag acgaatgtta
    10261 caatttactg gcaataaagt tttggataga ccttaatgag gacattatta agcctcatat
    10321 gttaattgct gcaagcaacc tccagtggcg accagaatcc aaatcaggcc ttcttacttt
    10381 atttgctgga gatttttctg tgttttctgc tagtccaaaa gagggccact ttcaagagac
    10441 attcaacaaa atgaaaaata ctgttgagaa tattgacata ctttgcaatg aagcagaaaa
    10501 caagcttatg catatactgc atgcaaatga tcccaagtgg tccaccccaa ctaaagactg
    10561 tacttcaggg ccgtacactg ctcaaatcat tcctggtaca ggaaacaagc ttctgatgtc
    10621 ttctcctaat tgtgagatat attatcaaag tcctttatca ctttgtatgg ccaaaaggaa
    10681 gtctgtttcc acacctgtct cagcccagat gacttcaaag tcttgtaaag gggagaaaga
    10741 gattgatgac caaaagaact gcaaaaagag aagagccttg gatttcttga gtagactgcc
    10801 tttacctcca cctgttagtc ccgtttgtac atttgtttct ccggctgcac agaaggcatt
    10861 tcagccacca aggagttgtg gcaccaaata cgaaacaccc ataaagaaaa aagaactgaa
    10921 ttctcctcag atgactccat ttaaaaaatt caatgaaatt tctcttttgg aaagtaattc
    10981 aatagctgac gaagaacttg cattgataaa tacccaagct cttttgtctg gttcaacagg
    11041 agaaaaacaa tttatatctg tcagtgaatc cactaggact gcccccacca gttcagaaga
    11101 ttatctcaga ctgaaacgac gttgtactac atctctgatc aaagaacagg agagttccca
    11161 ggccagtacg gaagaatgtg agaaaaataa gcaggacaca attacaacta aaaaatatat
    11221 ctaaacccag ctttcttgta caaagtggct cgagcaggga tccgcccctc tccctccccc
    11281 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
    11341 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
    11401 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
    11461 aatgtcgtga aggaagcgta ggaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg
    11521 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    11581 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    11641 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    11701 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    11761 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    11821 ttttggaggc ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg
    11881 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
    11941 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
    12001 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
    12061 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
    12121 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
    12181 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
    12241 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
    12301 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
    12361 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
    12421 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
    12481 gaccgaaagg agcgcacgac cccatgcatc gataaaataa aagattttat ttagtctcca
    12541 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
    12601 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
    12661 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
    12721 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
    12781 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
    12841 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
    12901 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
    12961 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
    13021 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
    13081 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
    13141 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat
    13201 ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    13261 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    13321 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    13381 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    13441 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    13501 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    13561 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    13621 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    13681 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    13741 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    13801 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    13861 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    13921 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    13981 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    14041 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    14101 tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg caaatcaatc
    14161 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    14221 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    14281 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    14341 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    14401 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    14461 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    14521 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    14581 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    14641 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    14701 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    14761 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    14821 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    14881 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    14941 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    15001 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    15061 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    15121 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    15181 cctgcagcct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
    15241 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
    15301 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
    15361 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
    15421 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
    15481 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
    15541 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
    15601 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
    15661 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
    15721 gcaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt
    15781 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
    15841 actgacgagt tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc
    15901 cgtttttgtg gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat
    15961 gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt
    16021 tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt
    16081 tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact
    16141 cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg
    16201 gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac
    16261 gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag
    16321 gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa
    16381 gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct
    16441 cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga
    16501 tcctcccttt atccagccct cactccttct ctaggcgccg gccggatccc agtgtggtgg
    16561 tactaat
//
