LOCUS       sequence-190630-        9833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of GSK3A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: GSK3A_pLX307
FEATURES             Location/Qualifiers
     source          1..9833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1327..1351)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1359..1400
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1451..2039
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1504..1524)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1922..1933)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2111..2344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2422..2543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2459..2478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2513..2532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2583..2718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2738..2757)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2739..2757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2767..2784)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2767..2783)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2776..2798)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2925..3380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3012..3031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3222..3243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3406..3510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3511..3579,3580..4371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3729..3748)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4542..5130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5031..5050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5284..5301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5418..5439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5454..5459,5460..5477,5478..5484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5492..5508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5497..5519
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5516..5532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5516..5532
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5551..5571
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5553..5571
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5599..5825
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5826..6006
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6053..6178
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6671..6904
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7089..7133
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7364..7574
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7386..7582
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7433..7568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7614..8216
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7614..7633)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8110..8130
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8259..8376
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8514..9692
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8745..9683
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9640..9660
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    9712..9736
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcccaggcgg caccggcggc ggaaaggcat ctgtcggggc catgggtggg ggcgtcgggg
       61 cctcgagctc cgggggtgga cccggcggca gcggcggagg aggcagcgga ggccccggcg
      121 caggcactag cttcccgccg cccggggtga agctgggccg tgacagcggg aaggtgacca
      181 cagtcgtagc cactctaggc caaggcccag agcgctccca agaagtggct tacacggaca
      241 tcaaagtgat tggcaatggc tcatttgggg tcgtgtacca ggcacggctg gcagagacca
      301 gggaactagt cgccatcaag aaggttctcc aggacaagag gttcaagaac cgagagctgc
      361 agatcatgcg taagctggac cactgcaata ttgtgaggct gagatacttt ttctactcca
      421 gtggcgagaa gaaagacgag ctttacctaa atctggtgct ggaatatgtg cccgagacag
      481 tgtaccgggt ggcccgccac ttcaccaagg ccaagttgac catccctatc ctctatgtca
      541 aggtgtacat gtaccagctc ttccgcagct tggcctacat ccactcccag ggcgtgtgtc
      601 accgcgacat caagccccag aacctgctgg tggaccctga cactgctgtc ctcaagctct
      661 gcgattttgg cagtgcaaag cagttggtcc gaggggagcc caatgtctcc tacatctgtt
      721 ctcgctacta ccgggcccca gagctcatct ttggagccac tgattacacc tcatccatcg
      781 atgtttggtc agctggctgt gtactggcag agctcctctt gggccagccc atcttccctg
      841 gggacagtgg ggtggaccag ctggtggaga tcatcaaggt gctgggaaca ccaacccggg
      901 aacaaatccg agagatgaac cccaactaca cggagttcaa gttccctcag attaaagctc
      961 acccctggac aaaggtgttc aaatctcgaa cgccgccaga ggccatcgcg ctctgctcta
     1021 gcctgctgga gtacacccca tcctcaaggc tctccccact agaggcctgt gcgcacagct
     1081 tctttgatga actgcgatgt ctgggaaccc agctgcctaa caaccgccca cttccccctc
     1141 tcttcaactt cagtgctggt gaactctcca tccaaccgtc tctcaacgcc attctcatcc
     1201 ctcctcactt gaggtcccca gcgggcacta ccaccctcac cccgtcctca caagctttaa
     1261 ctgagactcc gaccagctca gactggcagt cgaccgatgc cacacctacc ctcactaact
     1321 cctccgaccc agctttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc
     1381 tcctcggtct cgattctacg tagtaatgaa ctagtnasca cccggtggcg catggacgcg
     1441 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     1501 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     1561 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     1621 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     1681 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     1741 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     1801 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     1861 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     1921 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     1981 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     2041 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     2101 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     2161 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     2221 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     2281 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     2341 agcagtacgt atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     2401 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     2461 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2521 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     2581 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     2641 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     2701 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     2761 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2821 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     2881 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     2941 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3001 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3061 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3121 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3181 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3241 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3301 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     3361 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3421 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3481 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3541 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3601 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3661 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3721 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3781 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3841 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3901 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3961 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4021 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4081 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4141 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4201 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4261 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4321 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4381 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4441 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4501 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4561 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4621 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4681 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4741 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4801 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4861 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4921 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4981 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5041 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5101 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5161 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5221 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5281 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     5341 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     5401 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     5461 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     5521 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     5581 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     5641 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     5701 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     5761 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     5821 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5881 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5941 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6001 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     6061 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     6121 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     6181 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     6241 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     6301 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     6361 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     6421 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     6481 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     6541 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     6601 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     6661 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     6721 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     6781 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     6841 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     6901 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     6961 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     7021 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     7081 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     7141 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     7201 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     7261 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct
     7321 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7381 aagcatgcgt ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7441 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7501 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7561 tggaggccta ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg
     7621 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     7681 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     7741 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     7801 aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg
     7861 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     7921 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     7981 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     8041 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     8101 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     8161 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa
     8221 ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     8281 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     8341 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     8401 gatccacttt ggcgccggct cgaggcctgc aggtgcaaag atggataaag ttttaaacag
     8461 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
     8521 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     8581 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     8641 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     8701 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     8761 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     8821 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     8881 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     8941 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     9001 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     9061 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     9121 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     9181 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     9241 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     9301 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     9361 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     9421 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     9481 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     9541 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     9601 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     9661 aaagtttttt tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg
     9721 tacaaaaaag caggcttcac catgagcggc ggcgggcctt cgggaggcgg ccctgggggc
     9781 tcgggcaggg cgcggactag ctcgttcgcg gagcccggcg gcggaggcgg agg
//
