LOCUS       sequence-190660-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes dCas9(N) fused to transmembrane tether.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: pTMt_NES_TCS(Q'G)_HA-dCas9(N)_P2A-Puro-WPRE
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     35..94
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             137..166
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             170..316
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             413..433
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             452..478
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cacttttttt caggttggac cggtgatatc caccatggag acagacacac tcctgctatg
       61 ggtactgctg ctctgggttc caggttccac tggtgaccat tctggtggcg gaagtggggg
      121 aggcagcggt cgacaagaac aaaaactcat ctcagaagag gatctgaatg ctgtgggcca
      181 ggacacgcag gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg tggtgatctc
      241 agccatcctg gccctggtgg tgctcaccat catctccctt atcatcctca tcatgctttg
      301 gcagaagaag ccacgtctgc agagcggcag cgagactccc gggacctcag agtccgccac
      361 acccgaaagt gctagcctag atttagctag cttaatcctc ggcaagcttg gcgaaaacct
      421 gtattttcag ggggggggag gcagcactag ttacccatac gacgtaccag attacgctgg
      481 aggttctgga tccgacaaga agtacagcat cggcctggcc atcggcacca actctgtggg
      541 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
      601 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
      661 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
      721 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
      781 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
      841 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
      901 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctg
//
