LOCUS       sequence-190661-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes dCas9(N) fused to transmembrane tether.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: pTMt_NES_TCS(Q'G)_HA-dCas9(N)_P2A-Puro-WPRE
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
       61 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg
      121 cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca
      181 tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct
      241 ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc
      301 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg
      361 ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc
      421 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc
      481 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc
      541 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga
      601 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca
      661 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg
      721 aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata
      781 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg
      841 tgtataacga gctgaccaa
//
