LOCUS       sequence-190668-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to transmembrane 
            tether.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: pTMt_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65...
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             122..271
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             299..352
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct
       61 gtctcagctg ggaggcgaca gccccaagaa gaagagaaag gtggaggcca gcggacgggc
      121 tgacgcattg gacgattttg atctggatat gctgggaagt gacgccctcg atgattttga
      181 ccttgacatg cttggttcgg atgcccttga tgactttgac ctcgacatgc tcggcagtga
      241 cgcccttgat gatttcgacc tggacatgct gattaacggt accgctagcg gcagtggaga
      301 gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc cagtgagcaa
      361 gcttatggct tcaaacttta ctcagttcgt gctcgtggac aatggtggga caggggatgt
      421 gacagtggct ccttctaatt tcgctaatgg ggtggcagag tggatcagct ccaactcacg
      481 gagccaggcc tacaaggtga catgcagcgt caggcagtct agtgcccaga agagaaagta
      541 taccatcaag gtggaggtcc ccaaagtggc tacccagaca gtgggcggag tcgaactgcc
      601 tgtcgccgct tggaggtcct acctgaacat ggagctcact atcccaattt tcgctaccaa
      661 ttctgactgt gaactcatcg tgaaggcaat gcaggggctc ctcaaagacg gtaatc
//
