LOCUS       sequence-190669-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to transmembrane 
            tether.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: pTMt_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65...
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     35..94
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             137..166
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             170..316
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             398..418
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             419..439
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             458..484
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cacttttttt caggttggac cggtgatatc caccatggag acagacacac tcctgctatg
       61 ggtactgctg ctctgggttc caggttccac tggtgaccat tctggtggcg gaagtggggg
      121 aggcagcggt cgacaagaac aaaaactcat ctcagaagag gatctgaatg ctgtgggcca
      181 ggacacgcag gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg tggtgatctc
      241 agccatcctg gccctggtgg tgctcaccat catctccctt atcatcctca tcatgctttg
      301 gcagaagaag ccacgtctgc agagcggcag cgagactccc gggacctcag agtccgccac
      361 acccgaaagt gctagccatg tcgaccatgc ggccgctgaa aacctgtatt ttcaggggcc
      421 caagaagaag aggaaggtgg ggggaggcag cactagttac ccatacgacg taccagatta
      481 cgctggaggt tctggatccg gcggtggctc gaagcccgcc ttcctgagcg gcgagcagaa
      541 aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa
      601 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga
      661 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga
      721 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact
      781 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga
      841 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc
//
