LOCUS       sequence-190678-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: 
            pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actttttttc aggttggacc ggtgccacca tggtcagcta ctgggacacc ggggtcctgc
       61 tgtgcgcgct gctcagctgt ctgcttctca caggatctag ttcaggttca aaattaaaag
      121 atcctgaact gagtttaaaa ggcacccagc acatcatgca agcaggccag acactgcatc
      181 tccaatgcag gggggaagca gcccataaat ggtctttgcc tgaaatggtg agtaaggaaa
      241 gcgaaaggct gagcataact aaatctgcct gtggaagaaa tggcaaacaa ttctgcagta
      301 ctttaacctt gaacacagct caagcaaacc acactggctt ctacagctgc aaatatctag
      361 ctgtacctac ttcaaagaag aaggaaacag aatctgcaat ctatatattt attagtgata
      421 caggtagacc tttcgtagag atgtacagtg aaatccccga aattatacac atgactgaag
      481 gaagggagct cgtcattccc tgccgggtta cgtcacctaa catcactgtt actttaaaaa
      541 agtttccact tgacactttg atccctgatg gaaaacgcat aatctgggac agtagaaagg
      601 gcttcatcat atcaaatgca acgtacaaag aaatagggct tctgacctgt gaagcaacag
      661 tcaatgggca tttgtataag acaaactatc tcacacatcg acaaaccaat acaatcatag
      721 atgtccaaat aagcacacca cgcccagtca aattacttag aggccatact c
//
