LOCUS       sequence-190681-       10347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of HSPA8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 10347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: HSPA8_pLX307
FEATURES             Location/Qualifiers
     source          1..10347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3259..3283)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3291..3332
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3382..3970
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3435..3455)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3853..3864)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4042..4275
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4353..4474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4390..4409)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4444..4463
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4514..4649
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4669..4688)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4670..4688)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4698..4715)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4698..4714)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4707..4729)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4856..5311
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4943..4962)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5153..5174
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5337..5441
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5442..5510,5511..6302)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5660..5679)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6473..7061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6962..6981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7215..7232
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7349..7370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7385..7390,7391..7408,7409..7415)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7423..7439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7428..7450
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7447..7463
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7447..7463
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7482..7502
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7484..7502
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7530..7756
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7757..7937
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7984..8109
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8602..8835
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9020..9064
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9295..9505
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9317..9513
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9364..9499
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9545..10147
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9545..9564)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10041..10061
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10190..10307
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgtccaaggg acctgcagtt ggtattgatc ttggcaccac ctactcttgt gtgggtgttt
     1381 tccagcacgg aaaagtcgag ataattgcca atgatcaggg aaaccgaacc actccaagct
     1441 atgtcgcctt tacggacact gaacggttga tcggtgatgc cgcaaagaat caagttgcaa
     1501 tgaaccccac caacacagtt tttgatgcca aacgtctgat tggacgcaga tttgatgatg
     1561 ctgttgtcca gtctgatatg aaacattggc cctttatggt ggtgaatgat gctggcaggc
     1621 ccaaggtcca agtagaatac aagggagaga ccaaaagctt ctatccagag gaggtgtctt
     1681 ctatggttct gacaaagatg aaggaaattg cagaagccta ccttgggaag actgttacca
     1741 atgctgtggt cacagtgcca gcttacttta atgactctca gcgtcaggct accaaagatg
     1801 ctggaactat tgctggtctc aatgtactta gaattattaa tgagccaact gctgctgcta
     1861 ttgcttacgg cttagacaaa aaggttggag cagaaagaaa cgtgctcatc tttgacctgg
     1921 gaggtggcac ttttgatgtg tcaatcctca ctattgagga tggaatcttt gaggtcaagt
     1981 ctacagctgg agacacccac ttgggtggag aagattttga caaccgaatg gtcaaccatt
     2041 ttattgctga gtttaagcgc aagcataaga aggacatcag tgagaacaag agagctgtaa
     2101 gacgcctccg tactgcttgt gaacgtgcta agcgtaccct ctcttccagc acccaggcca
     2161 gtattgagat cgattctctc tatgaaggaa tcgacttcta tacctccatt acccgtgccc
     2221 gatttgaaga actgaatgct gacctgttcc gtggcaccct ggacccagta gagaaagccc
     2281 ttcgagatgc caaactagac aagtcacaga ttcatgatat tgtcctggtt ggtggttcta
     2341 ctcgtatccc caagattcag aagcttctcc aagacttctt caatggaaaa gaactgaata
     2401 agagcatcaa ccctgatgaa gctgttgctt atggtgcagc tgtccaggca gccatcttgt
     2461 ctggagacaa gtctgagaat gttcaagatt tgctgctctt ggatgtcact cctctttccc
     2521 ttggtattga aactgctggt ggagtcatga ctgtcctcat caagcgtaat accaccattc
     2581 ctaccaagca gacacagacc ttcactacct attctgacaa ccagcctggt gtgcttattc
     2641 aggtttatga aggcgagcgt gccatgacaa aggataacaa cctgcttggc aagtttgaac
     2701 tcacaggcat acctcctgca ccccgaggtg ttcctcagat tgaagtcact tttgacattg
     2761 atgccaatgg tatactcaat gtctctgctg tggacaagag tacgggaaaa gagaacaaga
     2821 ttactatcac taatgacaag ggccgtttga gcaaggaaga cattgaacgt atggtccagg
     2881 aagctgagaa gtacaaagct gaagatgaga agcagaggga caaggtgtca tccaagaatt
     2941 cacttgagtc ctatgccttc aacatgaaag caactgttga agatgagaaa cttcaaggca
     3001 agattaacga tgaggacaaa cagaagattc tggacaagtg taatgaaatt atcaactggc
     3061 ttgataagaa tcagactgct gagaaggaag aatttgaaca tcaacagaaa gagctggaga
     3121 aagtttgcaa ccccatcatc accaagctgt accagagtgc aggaggcatg ccaggaggaa
     3181 tgcctggggg atttcctggt ggtggagctc ctccctctgg tggtgcttcc tcagggccca
     3241 ccattgaaga ggttgattac ccaactttct tgtacaaagt ggttgatatc ggtaagccta
     3301 tccctaaccc tctcctcggt ctcgattcta cgtagtaawg aamtattaga gtggtaataa
     3361 gatttaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     3421 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3481 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3541 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3601 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3661 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3721 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     3781 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3841 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3901 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3961 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     4021 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     4081 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4141 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4201 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4261 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     4321 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     4381 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4441 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     4501 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4561 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4621 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     4681 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4741 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4801 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4861 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4921 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4981 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5041 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5101 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5161 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5221 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5281 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5341 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5401 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5461 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5521 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5581 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5641 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5701 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5761 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5821 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5881 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5941 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6001 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6061 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6121 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6181 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6241 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6301 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6361 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6421 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6481 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6541 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6601 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6661 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6721 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6781 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6841 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6901 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6961 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7021 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7081 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7141 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7201 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7261 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7321 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7381 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7441 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     7501 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     7561 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     7621 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     7681 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     7741 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7801 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7861 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7921 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     7981 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8041 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8101 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8161 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8221 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8281 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8341 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8401 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8461 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8521 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8581 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8641 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8701 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8761 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8821 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     8881 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     8941 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9001 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9061 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9121 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9181 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     9241 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9301 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9361 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9421 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9481 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     9541 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     9601 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     9661 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     9721 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     9781 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9841 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9901 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9961 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
    10021 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
    10081 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
    10141 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
    10201 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    10261 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    10321 gggacagcag agatccactt tggcgcc
//
