LOCUS       sequence-190683-        9342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SLC25A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: SLC25A1_pLX307
FEATURES             Location/Qualifiers
     source          1..9342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2254..2278)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2286..2327
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2377..2965
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2430..2450)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2848..2859)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3037..3270
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3348..3469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3385..3404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3439..3458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3509..3644
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3664..3683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3665..3683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3693..3710)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3693..3709)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3702..3724)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3851..4306
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3938..3957)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4148..4169
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4332..4436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4437..4505,4506..5297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4655..4674)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5468..6056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5957..5976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6210..6227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6344..6365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6380..6385,6386..6403,6404..6410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6418..6434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6423..6445
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6442..6458
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6442..6458
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6477..6497
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6479..6497
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6525..6751
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6752..6932
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6979..7104
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7597..7830
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8015..8059
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8290..8500
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8312..8508
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8359..8494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8540..9142
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8540..8559)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9036..9056
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9185..9302
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgccggcgcc cggcgccccg cgcgctctgg cggccgccgc gcccgcgtcc gggaaggcca
     1381 agctgacgca cccggggaag gcgatcctgg caggcggcct ggcgggtggc atcgagatct
     1441 gcatcacctt ccccaccgag tacgtgaaga cgcagctgca gctggacgag cgctcgcacc
     1501 cgccgcggta ccggggcatc ggggactgcg tgcggcagac ggttcgcagc catggcgtcc
     1561 tgggcctgta ccgcggcctt agctccctgc tctacggttc catccccaag gcggccgtca
     1621 ggtttggaat gttcgagttc ctcagcaacc acatgcggga tgcccaggga cggctggaca
     1681 gcacgcgtgg gctgctgtgc ggcctgggcg ctggcgtggc cgaggccgtg gtggtcgtgt
     1741 gccccatgga gaccatcaag gtgaagttca tccacgacca gacctcccca aaccccaagt
     1801 acagaggatt cttccacggg gttagggaga ttgtgcggga acaagggctg aaggggacgt
     1861 accagggcct cacagccact gtcctgaagc agggctcgaa ccaggccatc cgcttcttcg
     1921 tcatgacctc cctgcgcaac tggtaccgag gggacaaccc caacaagccc atgaaccctc
     1981 tgatcactgg ggtcttcgga gctattgcag gcgcagccag tgtctttgga aacactcctc
     2041 tggatgtgat taagacccgg atgcagggcc tggaggcgca caaataccgg aacacgtggg
     2101 actgcggctt gcagatcctg aagaaggagg ggctcaaggc attctacaag ggcactgtcc
     2161 cccgcctggg ccgggtctgc ctggatgtgg ccatagtgtt tgtcatctat gatgaagtgg
     2221 tgaagctgct caacaaagtg tggaagacgg actacccaac tttcttgtac aaagtggttg
     2281 atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag aaaggaactt
     2341 aagtatctag tatccatgta aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     2401 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2461 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2521 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2581 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2641 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     2701 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     2761 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     2821 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     2881 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     2941 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     3001 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3061 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     3121 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3181 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3241 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     3301 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     3361 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3421 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     3481 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3541 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3601 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     3661 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     3721 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3781 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3841 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3901 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3961 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4021 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4081 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4141 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4201 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4261 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     4321 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4381 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4441 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4501 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4561 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4621 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4681 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4741 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4801 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4861 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4921 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4981 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5041 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5101 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5161 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5221 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5281 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5341 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5401 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5461 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5521 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5581 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5641 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5701 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5761 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5821 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5881 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5941 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6001 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6061 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6301 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6361 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6421 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     6481 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     6541 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     6601 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     6661 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     6721 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     6781 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6841 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6901 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     6961 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7021 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7081 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7141 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7201 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7261 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7321 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7381 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7441 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     7501 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     7561 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     7621 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     7681 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     7741 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     7801 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     7861 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     7921 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     7981 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     8041 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     8101 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     8161 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     8221 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8281 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     8341 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8401 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8461 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     8521 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8581 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     8641 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     8701 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     8761 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     8821 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     8881 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     8941 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     9001 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     9061 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     9121 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
     9181 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9241 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9301 ttcgggttta ttacagggac agcagagatc cactttggcg cc
//
