LOCUS       sequence-190686-       14944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of MED12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14944)
  AUTHORS   Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench JG, 
            Tirosh I, Piccioni F, Hartenian E, Horn H, Fagbami L, Root DE, Jaffe
            J, Lage K, Boehm JS, Hahn WC
  TITLE     Genetic and Proteomic Interrogation of Lower Confidence Candidate 
            Genes Reveals Signaling Networks in beta-Catenin-Active Cancers.
  JOURNAL   Cell Syst. 2016 Sep 28;3(3):302-316.e4. doi: 
            10.1016/j.cels.2016.09.001.
  PUBMED    27684187
REFERENCE   2  (bases 1 to 14944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.09"; journalName: "Cell Syst"; date: 
            "2016-09-28- 28"; volume: "3"; issue: "3"; pages: "302-316.e4"
COMMENT     Sequence Label: MED12_pLX307
FEATURES             Location/Qualifiers
     source          1..14944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7855..7879)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7887..7928
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7979..8567
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8032..8052)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8450..8461)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8639..8872
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8950..9071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8987..9006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9041..9060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9111..9246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9266..9285)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9267..9285)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9295..9312)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9295..9311)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9304..9326)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9453..9908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9540..9559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9750..9771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9934..10038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10039..10107,10108..10899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10257..10276)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11070..11658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11559..11578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11812..11829
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11946..11967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11982..11987,11988..12005,12006..12012)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12020..12036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12025..12047
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12044..12060
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12044..12060
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12079..12099
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12081..12099
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12127..12353
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12354..12534
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12581..12706
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13199..13432
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13617..13661
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        13892..14102
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        13914..14110
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      13961..14096
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             14142..14744
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(14142..14161)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     14638..14658
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    14787..14904
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tggcggcctt cgggatcttg agctacgaac accggcccct gaagcggccg cggctggggc
     1381 ctcccgatgt ttaccctcag gaccccaaac agaaggagga tgaactgacg gccttgaatg
     1441 taaaacaagg tttcaataac cagcctgctg tctctgggga tgagcatggc agtgccaaga
     1501 acgtcagctt caatcctgcc aagatcagtt ccaacttcag cagcattatt gcagagaaat
     1561 tacgttgtaa tacccttcct gacactggtc gcaggaagcc ccaagtgaac cagaaggata
     1621 acttctggct ggtgactgca cgatcccaga gtgccattaa cacttggttc actgacttgg
     1681 ctggcaccaa gccactcacg caactagcca aaaaggtccc cattttcagt aagaaggaag
     1741 aggtgtttgg gtacttagcc aaatacacag tgcctgtgat gcgggctgcc tggctcatta
     1801 agatgacctg tgcctactat gcagcaatct ctgagaccaa ggttaagaag agacatgttg
     1861 accctttcat ggaatggact cagatcatca ccaagtactt atgggagcag ttacagaaga
     1921 tggctgaata ctaccggcca gggcctgcag gaagtggggg ctgtggttcc acgatagggc
     1981 ccttgcccca tgatgtagag gtggcaatcc ggcagtggga ttacaccgag aagctggcca
     2041 tgttcatgtt tcaggatgga atgctggaca gacatgagtt cctgacctgg gtgcttgagt
     2101 gttttgagaa gatccgccct ggagaggatg aattgcttaa actgctgctg cctctgcttc
     2161 tccgatactc tggggaattt gttcagtctg catacctgtc ccgccggctt gcctacttct
     2221 gtacacggag actggccctg cagctggatg gtgtgagcag tcactcatct catgttatat
     2281 ctgctcagtc aacaagcacg ctacccacca cccctgctcc tcagccccca actagcagca
     2341 caccctcgac tccctttagt gacctgctta tgtgccctca gcaccggccc ctggtttttg
     2401 gcctcagctg tatcctacag accatcctcc tgtgctgtcc tagtgccttg gtttggcact
     2461 actcactgac tgatagcaga attaagaccg gctcaccact tgaccacttg cctattgccc
     2521 cgtccaacct gcccatgcca gagggtaaca gtgccttcac tcagcaggtc cgtgcaaagt
     2581 tgcgggagat cgagcagcag atcaaggagc ggggacaggc agttgaagtt cgctggtctt
     2641 tcgataaatg ccaggaagct actgcaggct tcaccattgg acgggtactt catactttgg
     2701 aagtgctgga cagccatagt tttgaacgct ctgacttcag caactctctt gactcccttt
     2761 gtaaccgaat ctttggattg ggacctagca aggatgggca tgagatctcc tcagatgatg
     2821 atgctgtggt gtcattgcta tgtgaatggg ctgtcagctg caagcgttct ggtcggcatc
     2881 gtgctatggt ggtagccaag ctcctggaga agagacaggc ggagattgag gctgagcgtt
     2941 gtggagaatc agaagccgca gatgagaagg gttccatcgc ctctggctcc ctttctgctc
     3001 ccagtgctcc cattttccag gatgtcctcc tgcagtttct ggatacacag gctcccatgc
     3061 tgacggaccc tcgaagtgag agtgagcggg tggaattctt taacttagta ctgctgttct
     3121 gtgaactgat tcgacatgat gttttctccc acaacatgta tacttgcact ctcatctccc
     3181 gaggggacct tgcctttgga gcccctggtc cccggcctcc ctctcccttt gatgatcctg
     3241 ccgatgaccc agagcacaag gaggctgaag gcagcagcag cagcaagctg gaagatccag
     3301 ggctctcaga atctatggac attgacccta gttccagtgt tctctttgag gacatggaga
     3361 agcctgattt ctcattgttc tcccctacta tgccctgtga ggggaagggc agtccatccc
     3421 ctgagaagcc agatgtcgag aaggaggtga agcccccacc caaggagaag attgaaggga
     3481 cccttggggt tctttacgac cagccacgac acgtgcagta cgccacccat tttcccatcc
     3541 cccaggagga gtcatgcagc catgagtgca accagcggtt ggtcgtactg tttggggtgg
     3601 gaaagcagcg agatgatgcc cgccatgcca tcaagaaaat caccaaggat atcttgaagg
     3661 ttctgaaccg caaagggaca gcagaaactg accagcttgc tcctattgtg cctctgaatc
     3721 ctggagacct gacattctta ggtggggagg atgggcagaa gcggcgacgc aaccggcctg
     3781 aagccttccc cactgctgaa gatatctttg ctaagttcca gcacctttca cattatgacc
     3841 aacaccaggt cacggctcag gtctcccgga atgttctgga gcagatcacg agctttgccc
     3901 ttggcatgtc ataccacttg cctctggtgc agcatgtgca gttcatcttc gacctcatgg
     3961 aatattcact cagcatcagt ggcctcatcg actttgccat tcagctgctg aatgaactga
     4021 gtgtagttga ggctgagctg cttctcaaat cctcggatct ggtgggcagc tacactacta
     4081 gcctgtgcct gtgcatcgtg gctgtcctgc ggcactatca tgcctgcctc atcctcaacc
     4141 aggaccagat ggcacaggtc tttgaggggc tgtgtggcgt cgtgaagcat gggatgaacc
     4201 ggtccgatgg ctcctctgca gagcgctgta tccttgctta tctctatgat ctgtacacct
     4261 cctgtagcca tttaaagaac aaatttgggg agctcttcag cgacttttgc tcaaaggtga
     4321 agaacaccat ctactgcaac gtggagccat cggaatcaaa tatgcgctgg gcacctgagt
     4381 tcatgatcga cactctagag aaccctgcag ctcacacctt cacctacacg gggctaggca
     4441 agagtcttag tgagaaccct gctaaccgct acagctttgt ctgcaatgcc cttatgcacg
     4501 tctgtgtggg gcaccatgat cccgataggg tgaatgacat cgcaatcctg tgtgcagagc
     4561 tgaccggcta ttgcaagtca ctgagtgcag aatggctagg agtgcttaag gccttgtgct
     4621 gctcctctaa caatggcact tgtggtttca acgatctcct ctgcaatgtt gatgtcagtg
     4681 acctatcttt tcatgactcg ctggctactt ttgttgccat cctcatcgct cggcagtgtt
     4741 tgctcctgga agatctgatt cgctgtgctg ccatcccttc actccttaat gctgcttgta
     4801 gtgaacagga ctctgagcca ggggcccggc ttacctgccg catcctcctt caccttttca
     4861 agacaccgca gctcaatcct tgccagtctg atggaaacaa gcctacagta ggaatccgct
     4921 cctcctgcga ccgccacctg ctggctgcct cccagaaccg catcgtggat ggagccgtgt
     4981 ttgctgttct caaggctgtg tttgtacttg gggatgcgga actgaaaggt tcaggcttca
     5041 ctgtgacagg aggaacagaa gaacttccag aggaggaggg aggaggtggc agtggtggtc
     5101 ggaggcaggg tggccgcaac atctctgtgg agacagccag tctggatgtc tatgccaagt
     5161 acgtgctgcg cagcatctgc caacaggaat gggtaggaga acgttgcctt aagtctctgt
     5221 gtgaggacag caatgacctg caagacccag tgttgagtag tgcccaggcg cagcgcctca
     5281 tgcagctcat ttgctatcca catcgactgc tggacaatga ggatggggaa aacccccagc
     5341 ggcagcgcat aaagcgcatt ctccagaact tggaccagtg gaccatgcgc cagtcttcct
     5401 tggagctgca gctcatgatc aagcagaccc ctaacaatga gatgaactcc ctcttggaga
     5461 acatcgccaa ggccacaatc gaggttttcc aacggtcagc agagacaggg tcatcttctg
     5521 gaagtactgc aagcaacatg cccagcagca gcaagaccaa gcctgtgctc agctctctag
     5581 agcgctctgg tgtatggctg gtggcccccc tcattgctaa actgcccacc tcagtccagg
     5641 gacatgtgtt aaaggctgct ggggaagaat tggagaaggg tcagcacctg ggttcctctt
     5701 cacgcaaaga acgtgatcga caaaagcaga agagcatgtc cctattgagc cagcagccct
     5761 tcttatcgct ggtgctaaca tgtctgaaag ggcaggatga acaacgcgag ggactcctta
     5821 cctccctcta cagccaggtg caccagattg tgaataattg gcgagatgac cagtacttag
     5881 atgattgcaa accaaagcag cttatgcatg aggcactcaa actgcggctc aacctggtgg
     5941 ggggcatgtt tgacacggtg cagcgcagca cccagcagac cacggagtgg gccatgctcc
     6001 tcctggagat catcatcagc ggcactgtcg acatgcagtc caacaatgag ctcttcacta
     6061 ctgtgttgga catgctgagc gtgctcatca atgggacatt ggctgcagac atgtctagca
     6121 tctcgcaagg tagcatggag gaaaacaagc gtgcatacat gaacctggcg aagaagttgc
     6181 agaaggagtt gggggagcgc cagtcagaca gtctggaaaa ggttcgccag ctgctgccac
     6241 tgcccaagca gacccgagat gtcatcacgt gtgagccaca gggctccctt atcgatacca
     6301 agggcaacaa gattgctggc ttcgattcca tcttcaagaa ggagggtcta caggtttcca
     6361 ccaaacagaa gatctcgccc tgggatcttt ttgaggggtt gaagccgtca gcaccactct
     6421 cttggggctg gtttggaaca gtccgagtgg accggcgagt ggctcgagga gaggagcagc
     6481 agcggttgct gctctaccac acacacctga ggccccggcc ccgcgcctat tacctggagc
     6541 cactgccact gcccccagaa gatgaggagc cgcctgctcc taccctgcta gagcctgaga
     6601 aaaaggctcc agagcccccc aaaactgaca aaccgggggc tgctccaccc agtactgagg
     6661 aacgcaagaa gaagtccacc aagggcaaga aacgcagcca gccagctacc aagacagagg
     6721 actatggaat gggcccgggt cggagcggcc cttatggtgt gacagtgcct ccggacctcc
     6781 tgcaccaccc aaaccctggt tctataacac accttaacta caggcaaggc tccataggcc
     6841 tgtacaccca gaaccagcca ctacctgcag gtggccctcg tgtggaccca taccgtcctg
     6901 tgcgcttacc aatgcagaag ctgcccaccc gaccaactta ccctggagtg ctgcccacaa
     6961 ccatgactgg cgtcatgggt ttagaaccct cctcttataa gacctctgtg taccggcagc
     7021 agcaacctgc ggtgccccaa ggacagcgcc ttcgccaaca gctccagcag agtcagggca
     7081 tgttgggaca gtcatctgtc catcagatga ctcccagctc ttcctacggt ttgcagactt
     7141 cccagggcta tactccttat gtttctcatg tgggattgca gcaacacaca ggccctgcag
     7201 gtaccatggt gccccccagc tactccagcc agccttacca gagcacccac ccttctacca
     7261 atcctactct tgtagatcct acccgccacc tgcaacagcg gcccagtggc tatgtgcacc
     7321 agcaggcccc cacctatgga catggactga cctccactca aaggttttca caccagacac
     7381 tgcagcagac acccatgata agtaccatga ctccaatgag tgcccagggc gtccaggcag
     7441 gcgtccgttc aacagccatc ctacctgagc agcagcagca gcagcaacag cagcaacagc
     7501 aacagcagca gcagcagcaa cagcaacagc agcagcagca gcagcagtac cacatccggc
     7561 agcagcagca gcagcagatc ctgcggcagc agcagcaaca gcaacagcag cagcagcagc
     7621 agcagcaaca gcaacagcag cagcagcaac agcaacaaca gcaacaccag cagcaacagc
     7681 agcaacaggc ggctcctccc caaccccagc cccagtccca gccccagttc cagcgccagg
     7741 ggcttcagca gacccagcag cagcaacaga cagcagcttt ggtccggcaa cttcaacaac
     7801 agctctctaa tacccagcca cagcccagta ccaacatatt tggacgctac agacccccaa
     7861 ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc ctcggtctcg
     7921 attctacgta gtaakgmact actaaaaccc ttcctcggaa ccgacgcgtt aagtcgacaa
     7981 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     8041 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     8101 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     8161 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     8221 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     8281 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     8341 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     8401 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     8461 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     8521 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     8581 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8641 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     8701 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8761 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8821 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     8881 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8941 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9001 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9061 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     9121 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9181 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9241 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     9301 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     9361 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     9421 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     9481 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9541 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9601 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9661 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9721 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9781 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9841 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     9901 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9961 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    10021 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
    10081 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
    10141 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
    10201 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
    10261 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
    10321 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
    10381 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
    10441 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    10501 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    10561 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
    10621 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    10681 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    10741 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    10801 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    10861 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    10921 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    10981 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    11041 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    11101 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    11161 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    11221 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    11281 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    11341 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    11401 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    11461 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    11521 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    11581 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    11641 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    11701 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    11761 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    11821 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    11881 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    11941 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    12001 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    12061 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
    12121 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
    12181 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
    12241 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
    12301 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
    12361 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    12421 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    12481 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
    12541 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
    12601 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
    12661 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
    12721 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
    12781 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
    12841 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
    12901 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
    12961 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
    13021 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
    13081 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
    13141 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
    13201 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
    13261 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
    13321 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
    13381 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
    13441 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
    13501 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
    13561 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
    13621 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
    13681 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
    13741 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
    13801 cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc
    13861 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct
    13921 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
    13981 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    14041 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    14101 cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca
    14161 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
    14221 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
    14281 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
    14341 cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
    14401 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
    14461 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
    14521 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
    14581 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
    14641 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
    14701 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg
    14761 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
    14821 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    14881 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
    14941 cgcc
//
