LOCUS       sequence-19070-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Peschiaroli A, Dorrello NV, Guardavaccaro D, Venere M, Halazonetis 
            T, Sherman NE, Pagano M
  TITLE     SCFbetaTrCP-mediated degradation of Claspin regulates recovery from 
            the DNA replication checkpoint response.
  JOURNAL   Mol Cell. 2006 Aug 4. 23(3):319-29.
  PUBMED    16885022
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Aug 4. 23(3):319-29."
COMMENT     Sequence Label: pcDNA3.1-FLAG-Claspin
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             109..132
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacttaagc ttaccatgga ctacaaggac
      121 gacgatgaca aggtaccagg atccacaggc gaggtgggtt ctgaggttca cctagaaatc
      181 aatgacccaa acgtcatttc acaagaggaa gcagatagtc cttcagatag tggacagggc
      241 agctatgaaa caattggacc cttgagtgaa ggagattcag atgaagagat atttgtaagt
      301 aagaagttga aaaacaggaa ggttctacaa gacagtgatt ccgaaacaga ggacacaaat
      361 gcctctccag agaaaactac ctatgacagt gccgaggagg aaaataaaga gaatttatat
      421 gctgggaaaa atacaaaaat caaaaggatt tacaaaactg tggcagacag tgatgaaagt
      481 tacatggaaa agtctttgta tcaggaaaat cttgaagcgc aagtgaaacc ttgcttagag
      541 ctgagtcttc agtctggaaa ctctacagac tttaccactg acagaaagag ttccaaaaag
      601 cacatacatg ataaagaagg aactgcagga aaagcaaaag taaaatcaaa aagaagactt
      661 gagaaagagg agagaaaaat ggaaaaaatt agacagctaa aaaagaagga aacaaaaaac
      721 caggaagatg atgtagaaca gccattttat gacagtggct gtcttcttgt gg
//
