LOCUS       sequence-190710-        6006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector for constitutive Venus expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pBabe NEO Venus
FEATURES             Location/Qualifiers
     source          1..6006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             21..490
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    555..754
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             955..1371
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1301..1323
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1339..1355
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             join(1382..1384,1385..1387,1388..2098)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1427..1448)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1688..1707)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2035..2056
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2158..2178)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2163..2492
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2343..2478
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2849..3643
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2903..2922)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3513..3532
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4166..4185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4447..4916
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(5031..5822,5823..5891))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5654..5673
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5892..5996)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgaaaagtgc cacctgcagc ctgaatatgg gccaaacagg atatctgtgg taagcagttc
       61 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
      121 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
      181 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
      241 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
      301 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
      361 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
      421 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
      481 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc
      541 caccaccggg aggcaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct
      601 atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg
      661 acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag acgtcccagg
      721 gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc
      781 ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct
      841 gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc
      901 atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag
      961 actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct
     1021 cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg
     1081 ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag
     1141 gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc
     1201 gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct
     1261 aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg
     1321 accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc cggccggatc
     1381 tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1441 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1501 cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac
     1561 cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
     1621 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1681 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1741 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1801 caagctggag tacaactaca atagccacaa cgtctatatc accgccgaca agcagaagaa
     1861 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
     1921 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1981 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2041 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagct
     2101 cgagggatcc cagtgtggtg gtacgtagga attcgccagc acagtggtcg accctgtgga
     2161 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2221 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     2281 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     2341 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     2401 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     2461 gaggcttttt tggaggccta ggcttttgca aaaagcttca cgctgccgca agcactcagg
     2521 gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg
     2581 accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag
     2641 aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac
     2701 agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa
     2761 agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga
     2821 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     2881 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     2941 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     3001 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     3061 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     3121 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     3181 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     3241 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     3301 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     3361 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     3421 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     3481 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     3541 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     3601 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     3661 gcatcgatgc atcgatgcgg cgggtgtggt ggttacgcgt aatctgctgc ttgcaaacaa
     3721 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3781 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3841 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3901 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3961 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4021 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4081 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4141 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4201 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4261 ggaaaaacgc cagcaacgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg
     4321 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     4381 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     4441 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     4501 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     4561 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     4621 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     4681 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     4741 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     4801 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     4861 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg
     4921 cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag
     4981 tgcggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5041 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     5101 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     5161 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5221 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5281 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5341 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5401 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5461 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5521 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5581 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5641 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5701 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5761 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5821 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5881 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5941 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6001 tttccc
//
