LOCUS       sequence-190744-         512 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli/S. cerevisiae amdS shuttle vector allowing cloning of 
            ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 512)
  AUTHORS   de Vries ARG, de Groot PA, van den Broek M, Daran JG
  TITLE     CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces 
            pastorianus.
  JOURNAL   Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 
            10.1186/s12934-017-0835-1.
  PUBMED    29207996
REFERENCE   2  (bases 1 to 512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-12-5"; volume: "16(1):222. doi"; pages: " 
            10.1186/s12934-017-0835-1"
COMMENT     Sequence Label: pUDP003
FEATURES             Location/Qualifiers
     source          1..512
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcaacatctt tggataatat cagaatgaga aagaacagat acgcagtacg ttttttggtg
       61 agctctttgc acttctttag ttctttccat caatatcagt tttttaaact tttaggacta
      121 aaagtgatgt ttaacttcaa aatgtttaaa attttgttct tcccgacgtt cattaagaat
      181 actaatacac tttaataatt attttaatat tttgttctaa ataatgactt ttaattaaaa
      241 aagataaaat ataaaaacat cataataact caccagaggt taagaacaaa aaaacaaatt
      301 agatatctgc taatccaata tagttaaatc aatctttcct tggtataatg ggtatattac
      361 atatatttca aggaccgaca ctcctaccaa atatctaaaa tttaccatat taacataaca
      421 tgtatataaa cgtcaaatca taatcagcac taacgtctca cggatcgtat atgccgtagc
      481 gacaatctaa gaactatgcg aggacacgct ag
//
