LOCUS       sequence-190746-        1647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli/S. cerevisiae amdS shuttle vector allowing cloning of 
            ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   de Vries ARG, de Groot PA, van den Broek M, Daran JG
  TITLE     CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces 
            pastorianus.
  JOURNAL   Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 
            10.1186/s12934-017-0835-1.
  PUBMED    29207996
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-12-5"; volume: "16(1):222. doi"; pages: " 
            10.1186/s12934-017-0835-1"
COMMENT     Sequence Label: pUDP003
FEATURES             Location/Qualifiers
     source          1..1647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgccacaat cttgggaaga attggctgct gacaagagag ctagattggc taagactatc
       61 ccagacgaat ggaaggttca aactttgcca gctgaagact ctgttatcga cttcccaaag
      121 aagtctggta tcttgtctga agctgaattg aagatcactg aagcttctgc tgctgacttg
      181 gtttctaagt tggctgctgg tgaattgact tctgttgaag ttactttggc tttctgtaag
      241 agagctgcta tcgctcaaca attgactaac tgtgctcacg aattcttccc agacgctgct
      301 ttggctcaag ctagagaatt ggacgaatac tacgctaagc acaaaagacc tgttggtcca
      361 ttgcacggtt tgccaatctc tttgaaggac caattgagag ttaagggtta cgaaacttct
      421 atgggttaca tctcttggtt gaacaagtac gacgaaggtg actctgtttt gactactatg
      481 ttgagaaagg ctggtgctgt tttctacgtt aagacttctg ttccacaaac tttgatggtt
      541 tgtgaaactg ttaacaacat catcggtaga actgttaacc caagaaacaa gaactggtct
      601 tgtggtggtt cttctggtgg tgaaggtgct atcgttggta tcagaggtgg tgttatcggt
      661 gttggtactg acatcggtgg ttctatcaga gttccagctg ctttcaactt cttgtacggt
      721 ttaagaccat ctcacggtag attgccatac gctaagatgg ctaactctat ggaaggtcaa
      781 gaaactgtac actctgttgt tggtccaatc actcactctg ttgaagactt gagattgttc
      841 actaagtctg ttttgggtca agaaccatgg aagtacgact ctaaggttat cccaatgcca
      901 tggagacaat ctgaatctga catcatcgct tctaagatca agaacggtgg tttgaacatc
      961 ggttactaca acttcgacgg taacgttttg ccacacccac caatcttgag aggtgttgaa
     1021 actactgttg ctgctttggc taaggctggt cacactgtta ctccatggac tccatacaag
     1081 cacgacttcg gtcacgactt gatctctcac atctacgctg ctgacggttc tgctgacgtt
     1141 atgagagaca tctctgcttc tggtgaacca gctatcccaa acatcaagga cttgttgaac
     1201 ccaaacatca aggctgttaa catgaacgaa ttgtgggaca ctcacttgca aaagtggaac
     1261 taccaaatgg aatacttgga aaagtggaga gaagctgaag aaaaggctgg taaggaattg
     1321 gacgctatca tcgctccaat cactccaact gctgctgtta gacacgacca attcagatac
     1381 tacggttacg cttctgttat caacttgttg gacttcactt ctgttgttgt tccagttact
     1441 ttcgctgaca agaacatcga caagaagaac gaatctttca aggctgtttc tgaattggac
     1501 gctttggttc aagaagaata cgacccagaa gcttaccacg gtgctccagt tgctgttcaa
     1561 gttatcggta gaagattgtc tgaagaaaga actttggcta tcgctgaaga agttggtaag
     1621 ttgttgggta acgttgttac tccataa
//
