LOCUS       sequence-190747-         218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli/S. cerevisiae amdS shuttle vector allowing cloning of 
            ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 218)
  AUTHORS   de Vries ARG, de Groot PA, van den Broek M, Daran JG
  TITLE     CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces 
            pastorianus.
  JOURNAL   Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 
            10.1186/s12934-017-0835-1.
  PUBMED    29207996
REFERENCE   2  (bases 1 to 218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-12-5"; volume: "16(1):222. doi"; pages: " 
            10.1186/s12934-017-0835-1"
COMMENT     Sequence Label: pUDP003
FEATURES             Location/Qualifiers
     source          1..218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcgaggaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat
       61 attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac
      121 atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg
      181 tgaatgctgg tcgctatact ggagctctcg agaaccct
//
