LOCUS       sequence-190756-         215 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y 
            P411T and expressing a ribozyme flanked g-RNA for Cas9 editing 
            targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 215)
  AUTHORS   de Vries ARG, de Groot PA, van den Broek M, Daran JG
  TITLE     CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces 
            pastorianus.
  JOURNAL   Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 
            10.1186/s12934-017-0835-1.
  PUBMED    29207996
REFERENCE   2  (bases 1 to 215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-12-5"; volume: "16(1):222. doi"; pages: " 
            10.1186/s12934-017-0835-1"
COMMENT     Sequence Label: pUDP012
FEATURES             Location/Qualifiers
     source          1..215
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           11..47
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        68..143
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           148..215
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
ORIGIN
        1 caaacaattc ctgatgagtc cgtgaggacg aaacgagtaa gctcgtcgaa ttgcttttct
       61 ctgatttgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      121 aaaaagtggc accgagtcgg tgcttttggc cggcatggtc ccagcctcct cgctggcgcc
      181 ggctgggcaa catgcttcgg catggcgaat gggac
//
