LOCUS       sequence-190759-         426 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUDP004 expressing a polycistronic g-RNA array for Cas9 editing 
            targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. 
            pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 426)
  AUTHORS   de Vries ARG, de Groot PA, van den Broek M, Daran JG
  TITLE     CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces 
            pastorianus.
  JOURNAL   Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 
            10.1186/s12934-017-0835-1.
  PUBMED    29207996
REFERENCE   2  (bases 1 to 426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-12-5"; volume: "16(1):222. doi"; pages: " 
            10.1186/s12934-017-0835-1"
COMMENT     Sequence Label: pUDP044
FEATURES             Location/Qualifiers
     source          1..426
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           1..37
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        58..133
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           138..205
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           222..258
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        279..354
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           359..426
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
ORIGIN
        1 ctgatgagtc cgtgaggacg aaacgagtaa gctcgtcgtg aataatattc tgagctagtt
       61 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
      121 accgagtcgg tgcttttggc cggcatggtc ccagcctcct cgctggcgcc ggctgggcaa
      181 catgcttcgg catggcgaat gggacacagc gcaaaattag gctgatgagt ccgtgaggac
      241 gaaacgagta agctcgtccc taatttgttg tgtatcttgt tttagagcta gaaatagcaa
      301 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcttttgg
      361 ccggcatggt cccagcctcc tcgctggcgc cggctgggca acatgcttcg gcatggcgaa
      421 tgggac
//
