LOCUS       sequence-190789-        8543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutant ULK2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Scudiero I, Mazzone P, D'Andrea LE, Ferravante A, Zotti T, Telesio 
            G, De Rubis G, Reale C, Pizzulo M, Muralitharan S, Vito P, Stilo R
  TITLE     CARMA2sh and ULK2 control pathogen-associated molecular patterns 
            recognition in human keratinocytes: psoriasis-linked CARMA2sh 
            mutants escape ULK2 censorship.
  JOURNAL   Cell Death Dis. 2017 Feb 23;8(2):e2627. doi: 10.1038/cddis.2017.51.
  PUBMED    28230860
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2017-02-23"; volume: "8(2):e2627. doi"; pages: " 
            10.1038/cddis.2017.51"
COMMENT     Sequence Label: pcDNA3-ULK2-K39I
FEATURES             Location/Qualifiers
     source          1..8543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             689..712
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3900..3917)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3906..4130
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4176..4604
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4263..4282)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4473..4494
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4613..4633)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4618..4947
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4798..4933
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4860..4879
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5014..5808
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5068..5087)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5678..5697
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5982..6103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6019..6038)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6073..6092
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6152..6168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6152..6168)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6165..6187)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6176..6192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6200..6206,6207..6224,6225..6230))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6245..6266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6383..6400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6554..7142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6634..6653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7313..8104,8105..8173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7936..7955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8174..8278)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8353..8372)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttaccatgga ctacaaggac gacgatgaca aggtacctaa
      721 ggatccagtg tggtggaatt ctgcagatat catggaggtg gtgggtgact tcgagtacag
      781 caagagggat ctcgtgggac acggggcctt cgccgtggtc ttccgggggc ggcaccgcca
      841 gaaaactgat tgggaggtag ctattataag tattaataaa aagaacttgt caaaatcaca
      901 aatactgctt ggaaaggaaa ttaaaatctt aaaggaactt cagcatgaaa atattgtagc
      961 actctatgat gttcaggaat tacccaactc tgtctttttg gtgatggagt attgcaatgg
     1021 tggagacctc gcagattatt tgcaagcgaa agggactctc agtgaagaca cgatcagagt
     1081 gtttctgcat cagattgctg ctgccatgcg aatcctgcac agcaaaggaa tcatccacag
     1141 agatctcaaa ccacagaaca tcttgctgtc ctatgccaat cgcagaaaat caagtgtcag
     1201 tggtattcgc atcaaaatag cggattttgg ttttgctcgt tacctacata gtaacatgat
     1261 ggctgcaaca ctgtgtggat ccccgatgta catggctcct gaggttatta tgtctcaaca
     1321 ttatgatgct aaggctgact tgtggagcat aggaacagtg atataccaat gcctagttgg
     1381 aaaaccacct tttcaggcca atagtcctca agacttaagg atgttttatg aaaaaaacag
     1441 gagcttaatg cctagtattc ccagagaaac atcaccttat ttggctaatc tccttttggg
     1501 tttgcttcag agaaaccaaa aagatagaat ggactttgaa gcgtttttta gccatccttt
     1561 tcttgagcaa ggtccagtaa aaaaatcttg cccagttcca gtgcccatgt attctggttc
     1621 tgtctctgga agctcctgtg gcagctctcc atcttgtcgt tttgcttctc caccatccct
     1681 tccagatatg cagcatattc aggaagaaaa cttatcttcc ccaccattgg gtcctcccaa
     1741 ctatctacaa gtttccaaag attctgccag tactagtagc aagaactctt cttgtgacac
     1801 ggatgacttt gttttggtgc cacacaacat ctcgtcagac cactcatgtg atatgccaat
     1861 ggggactgct ggcagacgtg cttcaaatga attcttggtg tgtggagggc agtgtcagcc
     1921 tactgtgtca cctcacagcg aaacagcacc aattccagtt cctactcaaa taaggaatta
     1981 tcagcgcata gagcagaatc ttacatctac tgccagctca ggcacaaatg tacatggttc
     2041 tccaagatct gcagtggtac gaaggtccaa caccagcccc atgggcttcc tccggccggg
     2101 atcatgctcc ccagtaccag cagacacagc acagacagtt ggacgaaggc tctccactgg
     2161 gtcttctagg ccttactcac cttccccttt ggttggtacc attcctgagc aattcagtca
     2221 gtgctgctgt gggcatcctc agggccatga ctccaggagt agaaactcct caggttctcc
     2281 agtgccacaa gctcagtccc cacagtctct cttatcgggt gctagactgc agagcgcccc
     2341 caccctcact gacatctatc agaacaagca gaagctcaga aaacagcact ctgaccccgt
     2401 gtgcccatcc catactgggg ctgggtacag ctactcgcct cagcccagtc ggcctggcag
     2461 ccttggaact tctcccacca agcacttggg gtcctctcca cggagttctg actggttctt
     2521 taaaactcct ttgccaacaa tcattggctc tcctactaag accacagctc ctttcaaaat
     2581 ccctaaaact caagcatctt ccaacctgtt agccttggtt actcgtcatg ggcctgctga
     2641 agaacagtcg aaagatggga atgagccacg ggaatgtgcc cattgcctct tagtgcaagg
     2701 aagtgagagg cagcgggccg agcagcagag caaggcagtg tttggcagat ctgtcagtac
     2761 cgggaagtta tcagatcaac aaggaaagac tcctatatgt cgacatcagg gcagcacaga
     2821 cagtttaaat acagaacgac caatggatat agctccggca ggagcctgtg gtggtgttct
     2881 ggcacctcct gcaggtacag cagcaagttc caaggctgtc ctcttcactg tagggtctcc
     2941 tccacacagt gcggcagccc ccacttgtac ccacatgttc cttcgaacaa gaacaacctc
     3001 agtggggccc agcaactccg ggggctctct ttgtgccatg agtggccgcg tgtgcgtggg
     3061 gtccccgcct ggcccaggct tcggctcttc ccctccagga gcagaggcag ctcccagcct
     3121 gagatacgtg ccttacggtg cttcaccccc cagcctagag gggctcatca cctttgaagc
     3181 ccctgaactg ccggaggaga cgctgatgga gcgggaacac acagacacct tacgccatct
     3241 gaatgtgatg ctgatgttca ctgagtgtgt gctggacctg acagccatga ggggaggaaa
     3301 ccctgagctg tgcacatctg ctgtgtcctt gtaccagatc caggagagtg tggtggtgga
     3361 ccagatcagt cagctgagca aagactgggg gcgggtggag cagctggtgt tgtacatgaa
     3421 agcagcacag ctgcttgcgg cttctctgca tcttgccaaa gcccagatca agtccgggaa
     3481 actgagccca tccacagctg tgaaacaagt tgtcaagaat ctgaacgaac gatataaatt
     3541 ctgcatcacc atgtgcaaga aacttacaga aaagctgaat cgattcttct ctgacaaaca
     3601 gaggtttatt gatgaaatca acagtgtgac tgcagagaaa ctcatctata attgtgctgt
     3661 agaaatggtt cagtctgcag ccctggatga gatgtttcag cagaccgaag atattgttta
     3721 tcgctatcat aaggcagccc ttcttttgga aggcctaagt aggattctac aggaccctgc
     3781 agatattgaa aatgtgcata aatataaatg tagtattgag agaagactgt cggcgctctg
     3841 ccatagcacc gcaaccgtgt gactcgagtc tagagggccc gtttaaaccc gctgatcagc
     3901 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     3961 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4021 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4081 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4141 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4201 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4261 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4321 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4381 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4441 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4501 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4561 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4621 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4681 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4741 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4801 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4861 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     4921 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     4981 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5041 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5101 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5161 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5221 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5281 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5341 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5401 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5461 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5521 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5581 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5641 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5701 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5761 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5821 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5881 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5941 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6001 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6061 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6121 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6181 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6241 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6301 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6361 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6421 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6481 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6541 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6721 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6901 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6961 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7021 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7081 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7201 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7321 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7381 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7441 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7501 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7561 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7621 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7681 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7741 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7801 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7861 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7921 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7981 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8041 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8101 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8161 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8221 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8281 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     8341 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     8401 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     8461 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     8521 tgtacgggcc agatatacgc gtt
//
