LOCUS       sequence-190807-        7530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutant CARD14/CARMA2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Scudiero I, Mazzone P, D'Andrea LE, Ferravante A, Zotti T, Telesio 
            G, De Rubis G, Reale C, Pizzulo M, Muralitharan S, Vito P, Stilo R
  TITLE     CARMA2sh and ULK2 control pathogen-associated molecular patterns 
            recognition in human keratinocytes: psoriasis-linked CARMA2sh 
            mutants escape ULK2 censorship.
  JOURNAL   Cell Death Dis. 2017 Feb 23;8(2):e2627. doi: 10.1038/cddis.2017.51.
  PUBMED    28230860
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2017-02-23"; volume: "8(2):e2627. doi"; pages: " 
            10.1038/cddis.2017.51"
COMMENT     Sequence Label: pcDNA3-CARMA2sh-delta458-500
FEATURES             Location/Qualifiers
     source          1..7530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             692..718
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2887..2904)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2893..3117
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3163..3591
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3250..3269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3460..3481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3600..3620)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3605..3934
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3785..3920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3847..3866
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4001..4795
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4055..4074)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4665..4684
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4969..5090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5006..5025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5060..5079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5139..5155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5139..5155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5152..5174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5163..5179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5187..5193,5194..5211,5212..5217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5232..5253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5370..5387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5541..6129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5621..5640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6300..7091,7092..7160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6923..6942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7161..7265)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7340..7359)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttaccatggg atacccatac gatgttccag attacgctgt
      721 ggtaccagga tccagtgtgg tggaattcat gggggaactg tgccgcaggg actccgcact
      781 cacggcactg gacgaggaga cactgtggga gatgatggag agccaccgcc acaggatcgt
      841 acgctgcatc tgccccagcc gcctcacccc atacctgcgc caggccaagg tgctgtgcca
      901 gctggacgag gaggaggtgc tgcacagccc ccggctcacc aacagcgcca tgcgggccgg
      961 gcacttgctg gatttgctga agactcgagg gaagaacggg gccatcgcct tcctggagag
     1021 cctgaagttc cacaaccctg acgtctacac cctggtcacc gggctgcagc ctgatgttga
     1081 cttcagtaac tttagcggtc tcatggagac atccaagctg accgagtgcc tggctggggc
     1141 catcggcagc ctgcaggagg agctgaacca ggaaaagggg cagaaggagg tgctgctgcg
     1201 gcggtgccag cagctgcagg agcacctggg cctggccgag acccgtgccg agggcctgca
     1261 ccagctggag gctgaccaca gccgcatgaa gcgtgaggtt agcgcacact tccatgaggt
     1321 gctgaggctg aaggacgaga tgctcagcct ctcgctgcac tatagcaatg cgctgcagga
     1381 aaaggagctg gccgcctcac gctgccgcag cctgcaggag gagctgtatc tactgaagca
     1441 ggagctgcag cgagccaaca tggtttcctc ctgtgagctg gaattgcaag agcagtccct
     1501 gaggacagcc agcgaccagg agtccgggga tgaggagctg aaccgcctga aggaggagaa
     1561 tgagaaactg cgctcgctga ctttcagcct ggcggagaag gacattctgg agcagagcct
     1621 ggacgaggcg cgggggagcc gacaggagct ggtggagcgc atccactcgc tgcgggagcg
     1681 ggccgtggct gccgagaggc agcgagagca gtactgggaa gagaaggaac agaccctgct
     1741 gcagttccag aagagtaaga tggcctgcca actctacagg gagaaggtga atgcgctgca
     1801 ggcccaggtg tgcgagctgc agaaggagcg agaccaggcg tactccgcga gggacagtgc
     1861 tcagagggag atttcccaga gcctggtgga gaaggactcc ctccgcaggc aggtgttcga
     1921 gctgacggac caggtctgcg agctgcgcac acagcttcgc cagctgcagg cagagcctcc
     1981 gggtgtgctc aagcaggaag ccaggaccag ggagccctgt ccacgggaga agcagcggct
     2041 ggtgcggatg catgccatct gccccagaga cgacagcgac tgcagcctcg tcagctccac
     2101 agagtctcag ctcttgtcga gctgcctgga gatcccggag ggagacccgg gagccctgcc
     2161 gggagctaag gcaggcgacc cacacctgga ttatgagctc ctagacacgg cagaccttcc
     2221 gcagctggaa agcagcctgc agccagtctc ccctggaagg cttgatgtct cggagagcgg
     2281 cgtcctcatg cggcggaggc cagcccgcag gatcctgagc caggtcacca tgctggcgtt
     2341 ccagggggat gcattgctgg agcagatcag cgtcatcggc gggaacctca cgggcatctt
     2401 catccaccgg gtcaccccgg gctcggcggc ggaccagatg gccttgcgcc cgggcaccca
     2461 gattgtgatg gttgattacg aagcctcaga gcccttgttc aaggcagtcc tggaggacac
     2521 gaccctggag gaggccgtgg ggcttctcag gagggtggac ggcttctgct gcctgtctgt
     2581 gaaggtcaac acggacggtt ataagaggct actccaggac ctggaggcca aagtggcgac
     2641 ctcgggggac tcattctaca tccgggtcaa cctggccatg gagggcaggg ccaaagggga
     2701 gctgcaggtg cattgcaacg aggtcctgca cgtcaccgac accatgttcc agggctgcgg
     2761 ctgctggcat gcccaccgcg tgaactctta caccatgaag gatactgccg cgcacggcac
     2821 catccccaac tactccaggt gagcggccgc tcgagtctag agggcccgtt taaacccgct
     2881 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     2941 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3001 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3061 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3121 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3181 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3241 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3301 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3361 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3421 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3481 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3541 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     3601 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3661 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3721 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3781 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3841 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     3901 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     3961 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4021 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4081 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4141 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     4201 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4261 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4321 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4381 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4441 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4501 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     4561 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4621 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4681 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     4741 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     4801 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     4861 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     4921 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     4981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     5101 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     5161 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5221 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5281 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     6841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     6901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7261 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     7321 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     7381 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     7441 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     7501 ttcgcgatgt acgggccaga tatacgcgtt
//
