LOCUS       sequence-190840-       12067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  caCas9 integrating vector into the Neut5L locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12067)
  AUTHORS   Shapiro RS, Chavez A, Porter CBM, Hamblin M, Kaas CS, DiCarlo JE, 
            Zeng G, Xu X, Revtovich AV, Kirienko NV, Wang Y, Church GM, Collins 
            JJ
  TITLE     A CRISPR-Cas9-based gene drive platform for genetic interaction 
            analysis in Candida albicans.
  JOURNAL   Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. 
            Epub 2017 Oct 23.
  PUBMED    29062088
REFERENCE   2  (bases 1 to 12067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-017-0043-0"; journalName: "Nat Microbiol"; date: 
            "2018-01"; volume: "3"; issue: "1"; pages: "73-82"
COMMENT     Sequence Label: pJK-caCas9-NatMX-Neut5L
FEATURES             Location/Qualifiers
     source          1..12067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..4104
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1..4104
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4117..4137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     4142..4158
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4143..4159
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(5095..5292)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(5893..6236)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(7199..7218)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7200..7218)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7225..7242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7225..7241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7234..7256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7470..7489)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8685..8706
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8869..8973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8974..9042,9043..9834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9192..9211)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10005..10593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10494..10513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10747..10764
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10881..10902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10917..10922,10923..10940,10941..10947)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10955..10971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10960..10982
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10979..10995
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10979..10995
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        11016..11034
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atggataaaa aatactccat aggtttggac attggaacca attcagttgg atgggctgta
       61 atcactgatg aatataaggt tccttctaaa aagttcaaag ttttaggaaa tactgataga
      121 cactccatta aaaagaattt aattggtgcc ttgttgtttg acagtggtga aacagctgaa
      181 gcaactcgtt tgaaaagaac agctagaaga agatatacca gaagaaagaa cagaatttgt
      241 tacttgcaag aaatcttttc taatgaaatg gcaaaggttg atgattcttt ttttcataga
      301 ttggaagaaa gtttcttagt tgaagaagat aagaaacatg aaagacatcc aatctttggt
      361 aatattgtag atgaagtggc ttaccacgaa aaatatccta caatttatca tttgagaaaa
      421 aaattagttg actccactga taaagctgac ttaagactta tatatttggc attggctcac
      481 atgatcaagt tcagaggtca cttcttaatt gaaggtgatt tgaatccaga taattccgat
      541 gtcgacaagt tgtttattca attggttcaa acttacaacc aacttttcga ggaaaatcca
      601 atcaatgcct caggtgtgga tgctaaagct attttatctg ctagattgtc aaaaagtaga
      661 cgtttggaaa atcttattgc ccaattgcca ggtgaaaaaa aaaatggatt gtttggaaac
      721 ttaattgcat tatctttggg tttgacccca aatttcaaat caaactttga tttggccgag
      781 gatgctaaac ttcaattgtc taaggataca tacgatgacg atttagataa tcttcttgcc
      841 caaattggtg atcaatatgc agatttattc ttggcagcta agaacttgag tgatgcaatt
      901 ttgttaagtg acattttgag agtcaacaca gaaattacaa aagctccttt atccgcctct
      961 atgattaaaa gatatgatga acaccatcaa gatttaacct tgttgaaagc tcttgtacgt
     1021 caacaattac cagaaaagta taaagaaata ttctttgatc aatctaaaaa tggttacgct
     1081 ggttatattg atggtggtgc tagtcaagaa gaattttata agtttatcaa gccaattctt
     1141 gaaaaaatgg atggtactga ggaattgttg gttaagttga atcgagagga tttacttcgt
     1201 aagcaaagaa catttgataa cggttctatc cctcatcaaa tccacttagg tgagttacac
     1261 gctatcttga gacgtcagga agatttttac ccttttttga aagacaatcg tgaaaaaatt
     1321 gaaaaaattt tgactttcag aattccttat tacgtcgggc cattggctag aggtaattct
     1381 agatttgcat ggatgactcg taaatctgaa gagactatca ccccatggaa ttttgaggaa
     1441 gttgttgata agggagcctc cgctcaatca ttcatcgaga gaatgaccaa tttcgacaaa
     1501 aacttgccaa atgagaaggt gttacctaaa cattctttgt tatatgaata tttcactgtt
     1561 tataatgaat taacaaaagt caaatacgtc actgaaggta tgagaaaacc agcattctta
     1621 agtggtgaac aaaagaaagc tatcgttgac ttgttattca aaactaatcg taaagtcact
     1681 gtcaaacaac ttaaagagga ctattttaag aaaatagagt gtttcgattc cgttgaaatc
     1741 agtggggttg aggatagatt taatgctagt ttaggaactt atcacgattt gttgaaaatt
     1801 atcaaagaca aagatttttt ggataatgaa gaaaacgaag atatcttgga ggacattgta
     1861 ttgactttga ctttgtttga ggatagagaa atgatcgaag aacgtttaaa aacttacgct
     1921 cacttattcg acgataaagt catgaaacaa ttaaaaagaa gaagatatac cggttgggga
     1981 agattatcta gaaagttgat taatggtatt agagataagc aaagtggtaa aaccatctta
     2041 gatttcctta aaagtgatgg ttttgcaaac agaaacttta tgcagttgat tcatgatgat
     2101 agtttgacct ttaaggaaga tattcaaaaa gctcaggttt ctggacaggg tgattcattg
     2161 catgaacata ttgccaactt agcagggtct ccagctatca aaaagggaat tttacaaact
     2221 gttaaagttg ttgatgagtt ggtgaaagtt atgggacgtc ataagcctga aaatatagtt
     2281 atagaaatgg ctagagaaaa tcaaacaact cagaaaggac aaaagaattc tcgtgaaaga
     2341 atgaaaagaa tagaagaagg aattaaagag ttgggttctc aaatcttaaa agaacaccca
     2401 gtcgaaaaca cccagttaca gaacgagaag ctttacttgt actatttgca aaacgggcgt
     2461 gacatgtatg tagatcaaga gttggatatc aatagacttt cagattacga tgtcgatcac
     2521 atagttcctc agtcattttt aaaagatgat tccattgaca acaaagtttt aacaagatct
     2581 gataagaata gaggtaaatc agacaatgtg ccatcagaag aagtggttaa gaaaatgaag
     2641 aactattgga gacaattgtt aaatgcaaag ttgattactc agagaaaatt tgacaactta
     2701 actaaagctg aaagaggagg attatccgaa ttagataaag ctggattcat aaaacgtcag
     2761 ttggttgaaa caagacaaat tactaaacat gtagcccaaa ttttagattc ccgaatgaat
     2821 actaaatatg atgaaaatga caaattaatc agagaggtca aagtgatcac attaaagtca
     2881 aagttggtgt cagatttccg aaaggatttt caattctaca aggtgagaga gatcaacaat
     2941 tatcatcatg ctcacgacgc atatttgaat gcagtggtag gaactgcctt aataaagaag
     3001 tatcctaagt tagaatccga attcgtgtat ggtgattata aagtctacga tgtcagaaag
     3061 atgatagcaa aatcagaaca agaaattggg aaagccaccg caaagtattt cttttactcc
     3121 aacatcatga atttctttaa gaccgaaatc accttggcta acggggaaat cagaaaacgt
     3181 cctttgattg aaacaaatgg agaaaccggt gaaattgtgt gggataaggg gagagatttt
     3241 gctactgtta gaaaagtttt atcaatgcct caagttaata ttgtaaagaa gactgaagtt
     3301 caaactggtg gtttctctaa ggaatccatt cttccaaaac gaaactcaga caagttgatc
     3361 gcccgaaaaa aagattggga tcctaagaaa tacggaggtt ttgattctcc tactgtcgca
     3421 tattctgtct tggtcgtggc taaagttgaa aagggtaaat ccaaaaaatt gaaatctgtg
     3481 aaagaattgt taggtattac catcatggag cgttcatcct ttgagaaaaa cccaattgac
     3541 tttctcgaag cgaagggcta taaagaagtg aagaaagatt tgattattaa gttgccaaag
     3601 tattctctct ttgaacttga aaatgggaga aagagaatgt tggcttcagc tggagaattg
     3661 caaaaaggaa acgagttagc tttgccttca aagtacgtta atttcttata cttagcatct
     3721 cattatgaaa agttgaaggg gtcccctgaa gacaatgagc aaaagcaatt attcgtagaa
     3781 caacataaac actatttgga tgagataatt gaacaaatct ccgagttttc taaaagagtt
     3841 attttggctg atgcaaattt ggataaagtt ttatctgctt ataataagca tcgtgacaaa
     3901 ccaattcgag aacaagctga aaatattatc cacttgttca cattgactaa cttgggagct
     3961 ccagccgcgt ttaagtactt tgacaccacc attgaccgta agagatatac ttcaacaaaa
     4021 gaagtgttag acgccacatt gattcatcag tccataactg gtttatatga aactagaatc
     4081 gatttgtcac aattgggggg tgatagccga gcggatccta agaaaaaacg aaaagtctga
     4141 ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccgag
     4201 tgaaattctg gaaatctgga aatctggttt tgtattcttg ttattcttct ttttgttatt
     4261 acatatataa cttgttactt ttttaaaaaa atctttgttt attttataaa tatataaaac
     4321 taaatttaag aaaaagagaa aaatgtttta tttgagagat tgaaatttta cttgaattta
     4381 gcttagcttt tataaagtat tattatgtaa aaaaacaaaa caaatataca ttaaaaagtt
     4441 aagactataa aatagccacc caaggcattt ctatatcttg ttgttgttgt tttcatcttc
     4501 tgtatcagag gaacttattt tattattttc gtcacgggta ttttctcttg tttgatgatt
     4561 catcccattc attccatcat aaaattctag aaggaccacc tttgattgta aatagtaata
     4621 attaccaccc ttatttaatt atttatttaa cttatttatt tatttattat acatatatac
     4681 aaatctaata aagtgaaaat ctcccccttc acatttataa ttggccagtt tttttcaaat
     4741 aagcattcca accagcatct ctataccttt taccttcaat atctggatct cttcctttac
     4801 caaacaatcc tctaccaaca atgataatat cagttccagt gctaacaact tcatcaacag
     4861 ttctatattg ttgtcctaat ccatcacctt tatcatctaa tccaactcca ggtgtcataa
     4921 taagccaatc aaatccttct tcttgtccac ccatatcacg ttgggcaata aatccaataa
     4981 caaattcctt atcggattta gcaatttcaa cagttttttg agaatattct ccatacgaat
     5041 tcgagctcgt ttaaacgtcg acactggatg gcggcgttag tatcgaatcg acagcagtat
     5101 agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac ttaacttcgc
     5161 atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa
     5221 tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa caagaatctt
     5281 tttattgtca gtactgactc gagttattat ggacatggca tagacatata caaagcttgt
     5341 tcaccatcgg aagcagtacc atcgtataaa gcagtatcca aaccacacaa agtgaaaccc
     5401 attcttctat aagcatgaat agctggagca ttaacattgg taacttccaa ccacaaatga
     5461 ccagcacctc tttctctggc gaattcagta gccaaaccca tcaaagctct accaacacca
     5521 tgacctctat gttctggagc aacttcaata tcttcaacag tcaatcttct gttccaacca
     5581 gaataagaaa caacaacgaa accagccaaa tcaccatcat caccataagc aacgaaagtt
     5641 ctagaatctg gatcaccatc ttcaccagca tcggattcat catcggattc atcatctggg
     5701 aaaaccttag tcaatggtgg atcaactgga acttctctca aagtgaaacc atcaccagta
     5761 gcagtaactc taaaaacagt atcggtagtg aaagaaccat ccaaagcttc aatagcttca
     5821 gcatcacctg gaacagaagt tctgtatcta taagcagtat catccaaagt agtagacata
     5881 attgtaggat ccggttgttt atgttcggat gtgatgtgag aactgtatcc tagcaagatt
     5941 ttaaaaggaa gtatatgaaa gaagaacctc agtggcaaat cctaaccttt tatatttctc
     6001 tacaggggcg cggcgtgggg acaattcaac gcgtctgtga ggggagcgtt tccctgctcg
     6061 caggtctgca gcgaggagcc gtaatttttg cttcgcgccg tgcggccatc aaaatgtatg
     6121 gatgcaaatg attatacatg gggatgtatg ggctaaatgt acgggcgaca gtcacatcat
     6181 gcccctgagc tgcgcacgtc aagactgtca aggagggtat tctgggcctc catgtcgctg
     6241 gccgggtgac ccggcgggga cgaggcaagc ttgatatgct aatgatccca ctgatgataa
     6301 ttcagctaac atcaataacc ctcttggctc ttggttggtg gtggtttctt tagctccctg
     6361 ttttaatcct tcaactactc cattcccagt gacaccatga gcattagtaa tatctgccca
     6421 actactaatt ttataaactc caccaatata ttgtttcttc acggtattac caatatcagc
     6481 aaattttcta tcttcaaaaa tcataaattg atgtttacgt gaaagttcta ataatggttc
     6541 aatagtggat tcataggaaa aatcattgat tatatcaata tgagtcttga ttaagcatac
     6601 ataaggaccc aatttatcaa ttaattcaag gaattcctta gtggtatcaa catcaattga
     6661 tgcacataaa ttggttttct tcagttccat taatcgaaat aatcgttgtg ctactggtga
     6721 ggcatgagtt tctgctctct cactataggt cttagtgttg actgtcatat cttttgtttg
     6781 ctatttctat ttctatttct ttcttcaact gtaaaagggg cgatatataa accagtgtgt
     6841 atggggttgt tgctcaggaa ttgatcaatg tttaaaagaa tggttgaaat catcattgaa
     6901 gaacaaaaaa aaagattttt tctttcatta tgttatctgt ataatatgca ggaaataaga
     6961 ttgcaaaaaa atagccgttt ttttggatac tatcaaacaa gaggaatgac taatacagaa
     7021 ttagtgttac gaatcaatgg cactacagca actttcaaca aaataatcta cgttcaaaat
     7081 ataatatata ctattaaata aaatcaaata ataaaataat tttaaagaaa gtagtaccag
     7141 ctcttttttt tgtttccgtt tataccatcc aaatcaattc ctattagtct ccaattcgcc
     7201 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7261 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7321 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7381 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7441 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7501 cacgttcgcc ggctaaacaa gtggtattca agcacaattc agttttctgg atctctaata
     7561 attgcaattg caattgcttc acatactctg tttataattc agtttctttc tcttgatgtt
     7621 actgatatat taagttaata aataccaata actttttttc aaaaatcttc tattttcctt
     7681 tcaattgatt atttaaacac gaatcgttaa taagctgtga ttgctttttc tctttctttt
     7741 tcttttcttt gttcaagttt gaaaaaaaaa aaaaaaggtt tctcttgttc gatgtagaat
     7801 tcagtgggtt atagttgtaa tgatatatgg aataaaaaga aagaaagaga agagaaagag
     7861 aagaagagaa gaattaagta aacgtgtatt agaatttaaa aattaaaatt catgataatc
     7921 tttaataata attaactaag aatcacaaaa ttgaaaattt gaaagaaaaa agatcactcc
     7981 aaggtcgtgt caagtgacta gaaattctta attaaaatag ttataacaaa aaaagaaatt
     8041 tgtatataac aattaagtaa aagaaaaaaa aaagcaattc gctttgtata ataataccca
     8101 gaaaaatata ttcatatatt cataaatcaa acatgaaatg aagaatgctg aatcacttga
     8161 taggatttag ttccattatg gtactaagaa gtaatattac ttctatgaaa aggttgaatc
     8221 cgagatcaaa attcatcttt tcatatactc tggagaaaaa aaaaaagtaa ttgtagtaag
     8281 aatgacaagt atcagttgag aaaaaaagta acaaagagaa agctcggagg aggctcccca
     8341 aaggttttat caccaatggt ggtaccacta acccaagaac agaaaaagca ttgatatttt
     8401 gaaaaataca atactcatct tttaacccaa accttttctt gtcctttaaa acttttatct
     8461 catgtctgtc caaagtcata ttgtcttact actttctctc cttcatcgtc cttccggctt
     8521 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8581 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8641 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8701 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8761 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8821 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     8881 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8941 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9001 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9061 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9121 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9181 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9241 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9301 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9361 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9421 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9481 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9541 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9601 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9661 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9721 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9781 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9841 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9901 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9961 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10021 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10081 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10141 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10201 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10261 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10321 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10381 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10441 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10501 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10561 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10621 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10681 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10741 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10801 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    10861 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    10921 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    10981 ggaaacagct atgaccatga ttacgccaag ctcgaaatta accctcacta aagggaacaa
    11041 aagctggtac cagagctatt aagatcacca gcctcgttta taataaactt agtctaatat
    11101 ttcttttgga ccaaaataga aactgttctc aggtggcaaa aaacagctta aagtctgtgt
    11161 taaaaaaaga tctcggctat gccaatcaaa aaggagtgac attattatgt aaaagaagaa
    11221 atgttgtaat tccgcgaaac aacaatttta atgcttactg tgccgccacc aatatcctat
    11281 ggccaagggg cctttaaaca tgccacggaa atatacagtc aaagtaaacc tactatgtct
    11341 ttagagcctt caggatcaat tgtttttaaa tgaatgactg caaaactaga aaagtagcct
    11401 cttacataag caagtcattt gtacaccgag agtttagtaa ccagtgaagc aagtatatct
    11461 ggcaaacctg aaacttatct gtgaattata aagctcataa gtaaaaagtg tagaaaagaa
    11521 gataaggcag accaaataca aatttgcaac agtacaacga atatttcagc gtcaaaacta
    11581 gagaataata aagaaaacga tcttttcaaa aagaaaaaac cttttagttt tcctttgttg
    11641 ttgttgtggg tgtgtgctat ttatattata tagtttactc ataataccat aaaatattcg
    11701 gtttgattag gttattttaa taagctaatt tgtttctaat cgtgtaattt atgctgtgta
    11761 tattaagtag tgtgtgcact gcccaaaaat gtttgttgtt tatagtcggt taaagagaaa
    11821 aaagaaaaaa agatccatac acacacgtta attagttgtt caacgtaata cactcatatt
    11881 ttgttcttat ttgctttcgg tcgctgttct caccaagatt tattgccaac gaaacaattt
    11941 ttttttatat attttcagat ttttcttttt ttcctttcct ttccttttct aattttcact
    12001 cctggttttc tttctttctt agaaacatta tctcgatatt aatattaaaa aaatataatc
    12061 attcaaa
//
