LOCUS       sequence-190845-        3446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low background cloning vector for conventional or homologous 
            recombination cloning with sticky blunt or TA compatible ends, 
            ampicillin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3446)
  TITLE     Jamorn Somana Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBS2ndd
FEATURES             Location/Qualifiers
     source          1..3446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..52
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     286..303
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    420..441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(456..461,462..479,480..486)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    494..510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     499..521
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     518..534
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     518..534
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     553..573
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        555..573
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     610..626
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     610..626
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1145..1161)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1146..1162)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1186..1205)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1187..1205)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1215..1232)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1215..1231)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1224..1246)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1373..1828
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1460..1479)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1670..1691
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1854..1958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1959..2027,2028..2819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2177..2196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2990..3446,1..132)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
       61 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      181 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      241 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      301 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      361 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      421 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      481 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      541 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt
      601 ggcggccgct ctagaactag tggatccccc gggctgcagg aattccagat ctcatatgga
      661 tatcaaatat atgactgtta ctgatctgaa tgatgcaggc gctactgtta ttggtacaat
      721 caagggtggt gaatggtttt taggaactcc gcataaagat attttatcta aacctggatt
      781 ttacttttta gtgagtaaac tcggtggtcc atttagtaat ccatgcgtat ctgcacgatt
      841 ttatgtaggt aatcagcgtt ctaagcaagg attcagtgcg gttttaagtc atattcgtca
      901 acgccggtct caacttgcgc gtactattgc aaataacaat gttccataca cagtgtttta
      961 tctgcctgct tctaagatga aacctctaac gacgggattt ggaaaaggtc agctagcttt
     1021 ggcgtttact cgtaatcatc attctgagta tcaaacactc gaagaaatga accgtatgtt
     1081 ggctgataac tttaaatttg ttttacaggc atattagata tccacgagat ctatggaagc
     1141 ttatcgatac cgtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg
     1201 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     1261 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     1321 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     1381 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     1441 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1501 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     1561 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     1621 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     1681 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     1741 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     1801 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     1861 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1921 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1981 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2041 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2101 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2161 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2221 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2281 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2341 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2401 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2461 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2521 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2581 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2641 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2701 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2761 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2821 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2881 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3121 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3421 acaggtatcc ggtaagcggc agggtc
//
