LOCUS       sequence-190853-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector with dox inducible expression of shRNA targeting 
            human FOXM1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  TITLE     FOXM1 toolbox
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Tet-pLKO-FOXM1-shRNA-84
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     165..185
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             396..629
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    701..822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(738..757)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     792..811
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      862..997
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1018..1036)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1046..1063)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1046..1062)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1055..1077)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1204..1659
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1291..1310)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1501..1522
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1685..1789
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1790..1858,1859..2650)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2008..2027)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2821..3409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3310..3329
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3563..3580
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3697..3718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3733..3738,3739..3756,3757..3763)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3771..3787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3776..3798
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3795..3811
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3795..3811
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3830..3850
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3832..3850
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3878..4104
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4105..4285
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4332..4457
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4950..5183
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5368..5412
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5701..5795
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     5712..5731
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    5749..5767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5777..5795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    5896..6013
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6062..6572
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6491..6509
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          6581..7153
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     7161..7180
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        7208..7226
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7237..7857
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(7310..7329)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    7892..8465
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8059..8076)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8286..8305
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8485..8816,1..268)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8485..8504)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
       61 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
      121 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
      181 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
      241 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
      301 aaggagcgca cgaccccatg catcggtacc tttaagacca atgacttaca aggcagctgt
      361 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
      421 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
      481 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      541 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      601 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
      661 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
      721 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      781 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
      841 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      901 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      961 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     1021 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1081 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1141 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1201 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1261 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1321 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1381 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     1441 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1501 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     1561 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     1621 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     1681 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1741 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1801 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1861 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1921 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1981 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2041 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2101 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2161 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2221 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2281 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2341 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2401 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2461 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2521 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2581 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2641 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2701 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2761 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2821 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2881 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2941 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3001 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3061 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3121 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3181 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3241 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3301 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3361 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3421 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3481 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3541 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     3601 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     3661 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     3721 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     3781 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     3841 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     3901 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     3961 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     4021 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     4081 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     4141 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4201 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4261 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     4321 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     4381 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     4441 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     4501 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     4561 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     4621 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     4681 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     4741 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     4801 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     4861 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     4921 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     4981 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     5041 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     5101 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     5161 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     5221 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     5281 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     5341 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     5401 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     5461 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     5521 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     5581 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     5641 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgca
     5701 atatttgcat gtcgctatgt gttctgggaa atcaccataa acgtgaaatc cctatcagtg
     5761 atagagactt ataagttccc tatcagtgat agagacaccg gttgcagggt ggtccgtgta
     5821 aactcgagtt tacacggacc accctgcaat ttttgaattc tcgacctcga gacaaatggc
     5881 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     5941 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     6001 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga
     6061 gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     6121 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     6181 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     6241 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     6301 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     6361 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     6421 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     6481 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     6541 tcgttgaccg aatcaccgac ctctctcccc agggggatct gtgagtttgg ggacccttga
     6601 ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag
     6661 ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg
     6721 tttctttcac tttctactct gttgacaacc attgtctcct cttattttct tttcattttc
     6781 tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt
     6841 ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat
     6901 attatattgt acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag
     6961 aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta
     7021 catcctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat
     7081 gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt
     7141 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     7201 agaattgtaa tacgactcac tatagggcga attgatatgt ctagattaga taaaagtaaa
     7261 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
     7321 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
     7381 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
     7441 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
     7501 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
     7561 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
     7621 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
     7681 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
     7741 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
     7801 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgcg
     7861 tacagcggct cccgggagtt ctagggatct gcccctctcc ctcccccccc cctaacgtta
     7921 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     7981 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     8041 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     8101 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     8161 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     8221 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     8281 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     8341 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     8401 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     8461 gataaggatc caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     8521 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     8581 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     8641 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     8701 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     8761 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaagg
//
