LOCUS       sequence-190855-       11303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cry2olig(E490G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Shin Y, Berry J, Pannucci N, Haataja MP, Toettcher JE, Brangwynne CP
  TITLE     Spatiotemporal Control of Intracellular Phase Transitions Using 
            Light-Activated optoDroplets.
  JOURNAL   Cell. 2017 Jan 12;168(1-2):159-171.e14. doi: 
            10.1016/j.cell.2016.11.054. Epub 2016 Dec 29.
  PUBMED    28041848
REFERENCE   2  (bases 1 to 11303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2017-01-12"; volume: "168(1-2):159-171.e14. doi"; pages: " 
            10.1016/j.cell.2016.11.054. Epub 2016 Dec 29"
COMMENT     Sequence Label: pHR-mCh-Cry2olig
FEATURES             Location/Qualifiers
     source          1..11303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1515..3008
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     misc_feature    3160..3748
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3213..3233)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3631..3642)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3751..3767)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3752..3768)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3858..4091
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4116..4134)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4117..4134)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4424..4443)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4657..4676)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4776..4798
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4836..4854)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4922..5026
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5027..5095,5096..5887)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5245..5264)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6058..6646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6547..6566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6800..6817
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6887..6907)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6892..7221
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7072..7207
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8355..8420
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8550..8570
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8995..9129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9032..9051)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9086..9105
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9297..9930
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9977..10102
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10599..10832
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11017..11061
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccatggtg tctaaaggcg aggaggataa catggctata atcaaggaat
      841 ttatgaggtt caaggtgcat atggagggat ctgtgaacgg tcacgagttc gaaatcgagg
      901 gcgaaggcga ggggcgaccc tatgaaggga cacagacggc caaactgaag gtgaccaagg
      961 gtggccccct gcctttcgcc tgggacatcc tgtcacccca gtttatgtac ggctctaagg
     1021 cttacgttaa gcaccctgcc gatattccgg actacttgaa actgagcttc cctgaagggt
     1081 tcaagtggga acgggtaatg aattttgagg atggcggtgt agtcacagtt acccaggaca
     1141 gctctcttca ggacggagaa tttatctata aggttaaact tcggggcact aacttcccat
     1201 cagacggccc cgtgatgcag aagaaaacta tgggctggga agccagctca gaacgcatgt
     1261 atcccgagga cggagcactg aagggagaaa tcaagcagcg actcaagctg aaggatgggg
     1321 gacattatga tgctgaggtc aaaaccacct ataaggccaa gaaacccgtt cagcttcctg
     1381 gggcatacaa cgtgaatatc aaactggata ttacatccca taacgaagac tataccatcg
     1441 tggaacagta cgagcgggcc gagggcagac atagcacagg gggcatggat gaattgtaca
     1501 aggcggccac gcgtatgaag atggacaaaa agactatagt ttggtttaga agagacctaa
     1561 ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt tttcctgtct
     1621 tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca agatggtgga
     1681 tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct gacctcactt
     1741 taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt accggtgcta
     1801 caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac cataccgtaa
     1861 aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat ctattgtatg
     1921 aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat tcttactgga
     1981 agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg cggttgatgc
     2041 caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta gggctggaga
     2101 atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca ggatggagca
     2161 atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat gcaaagaaca
     2221 gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat ttcggggaaa
     2281 taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg gcaagagata
     2341 agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt ttaagagagt
     2401 attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg ttgagtcatc
     2461 ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga caaggcagga
     2521 ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga tggatgcata
     2581 acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat
     2641 ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt gacatccttg
     2701 gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc ttggacaatc
     2761 ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa tggcttcccg
     2821 agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct ttaaccgtac
     2881 tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta gacatcgaca
     2941 cagctcgtga gctactagct aaagctattt caagaacccg tggagcacag atcatgatcg
     3001 gagcagcagc ccgggatcca ccggtcgcca ccggttcagg gagcggatcc ggctcaggga
     3061 gtgcggccgc aactcccacc tgcaacatgc gtgactgact gaggccgcga ctctagagtc
     3121 gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa
     3181 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3241 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3301 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3361 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3421 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3481 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3541 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3601 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3661 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3721 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc
     3781 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     3841 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     3901 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     3961 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4021 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4081 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
     4141 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
     4201 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
     4261 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
     4321 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
     4381 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     4441 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     4501 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     4561 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     4621 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     4681 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4741 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4801 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     4861 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     4921 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4981 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5041 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5101 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5161 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5221 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5281 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5341 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5401 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5461 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5521 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5581 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5641 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5701 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5761 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5821 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5881 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5941 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6001 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6061 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6121 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6181 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6241 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6301 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6361 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6421 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6481 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6541 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6601 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6661 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6721 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6781 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6841 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     6901 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6961 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7021 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7081 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7141 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7201 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     7261 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     7321 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     7381 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     7441 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     7501 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
     7561 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     7621 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     7681 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     7741 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     7801 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
     7861 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
     7921 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     7981 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     8041 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
     8101 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
     8161 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
     8221 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
     8281 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     8341 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     8401 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     8461 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     8521 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     8581 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     8641 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     8701 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
     8761 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
     8821 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
     8881 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     8941 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     9001 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9061 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9121 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     9181 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
     9241 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     9301 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     9361 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     9421 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     9481 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     9541 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     9601 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     9661 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     9721 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     9781 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9841 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9901 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     9961 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
    10021 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
    10081 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
    10141 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
    10201 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
    10261 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
    10321 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
    10381 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
    10441 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    10501 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    10561 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    10621 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    10681 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    10741 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    10801 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    10861 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    10921 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    10981 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    11041 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    11101 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    11161 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    11221 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    11281 gacagatcca ttcgattagt gaa
//
