LOCUS       sequence-190862-        7362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A FRET sensor for Gq activation, encoded on a single plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Mastop M, Bindels DS, Shaner NC, Postma M, Gadella TWJ Jr, Goedhart 
            J
  TITLE     Characterization of a spectrally diverse set of fluorescent proteins
            as FRET acceptors for mTurquoise2.
  JOURNAL   Sci Rep. 2017 Sep 20;7(1):11999. doi: 10.1038/s41598-017-12212-x.
  PUBMED    28931898
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-09-20"; volume: "7(1):11999. doi"; pages: " 
            10.1038/s41598-017-12212-x"
COMMENT     Sequence Label: Gq-sensor[mTq2-mNG]
FEATURES             Location/Qualifiers
     source          1..7362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     71..88
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     267..284
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(569..1273)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELY"
     regulatory      1009..1018
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1270..1279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1318..1521)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1347..1367)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1522..1825
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2000..2588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2080..2099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2917..2964
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2989..3008
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3196..3990)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3196..3990)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3307..3326)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3917..3936
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4025..4382)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4039..4174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4191..4382
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4339..4359
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(4384..4488)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4515..4970
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4602..4621)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4812..4833
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4977..5098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4988..5007)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5042..5061
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5957..5978)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(6113..6625,6626..6628,6629..6631))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     6306..6325
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6565..6586
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(7031..7039,7040..7042,7043..7362,1..255)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7204..7223)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gctacagacg ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt cacgacattc
       61 aacagacctt gcattccttt ggcgagaggg gaaagacccc taggaatgct cgtcaagaag
      121 acagggccag gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac
      181 atatagacaa acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg
      241 ggggagggag aggggggggc ggaattattt aggtgacact ataggggccc caattgagat
      301 ctgtcgactt cgaacctgca ggtccggagc atgcaggcct cgtacgccgc ggttaaagga
      361 tggcacagaa aaacttcttc tccctaaacg ggttttctga agccggaaca ggggtcagga
      421 gggggtcttc cttggcatgt gcttcacagt aggccatcaa atctgcagct gccttggaca
      481 cctttatcct gtcgatattg gcttccatct taagctgctc taccagcttc ctggcttgtg
      541 ctatgctggc ggtgttgttg ctggccatgt acagctcgtc catgcccatc acatcggtaa
      601 aggccttttg ccactccttg aagttgagct cggtcttgga gtgcttgagc tccgtcttac
      661 ggaacacgta catcggctgg ttcttcagat agttagccgc cattggcttg gcaaaggtgt
      721 aggtggtccg cgcagtgctc cggtagcgct tgccatttcc agtggtgtaa ctccacttaa
      781 aggtactgat gatggttttg tcgttggggt aagtcttctt cgacctgcac cagtccgcag
      841 cggtcagcga gttggtcatc acaggaccgt cagcagggaa accagtcccc ttcacctggg
      901 cctctccttt gatgtggctt ccctcgtagg tgtagcggta gttaacagta agggaggcac
      961 catcttcaaa ctgcattgtg cgatggactt ggtagccgga gccatctacc atggcggcct
     1021 ggaaaggcga catcccgtca gggtagggca ggtactgatg gaagccatac ccgatatgag
     1081 ggaccagaat ccagggggag aactggaggt cacccttggt ggacttcagg tttaactcct
     1141 cataaccatc atttggattg ccggtgccct gacccaccat gtcaaagtcc acaccgttga
     1201 tggagccaaa gatgtgtaac tcatgtgtcg ctgggagaga ggccatgtta tcctcctcgc
     1261 ccttgctcac catggtggcg accggtagcg ctagcggatc tgacggttca ctaaaccagc
     1321 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
     1381 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
     1441 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
     1501 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
     1561 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     1621 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     1681 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     1741 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     1801 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
     1861 cgtaattgat tactattaat aactaatgca tggcggtaat acggttatcc acagaatcag
     1921 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1981 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2041 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2101 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2161 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2221 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2281 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2341 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2401 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2461 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2521 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2581 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2641 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2701 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaacctgag gctatggcag
     2761 ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg
     2821 gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga
     2881 cagagtgcca gccctgggac cgaaccccgc gtttatgaac aaacgaccca acaccgtgcg
     2941 ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc
     3001 gtgtttcagt tagcctcccc ctagggtggg cgaagaactc cagcatgaga tccccgcgct
     3061 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
     3121 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
     3181 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     3241 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     3301 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     3361 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     3421 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa
     3481 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     3541 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     3601 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     3661 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     3721 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     3781 cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg acaggtcggt
     3841 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     3901 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     3961 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
     4021 gatctttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     4081 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     4141 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     4201 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     4261 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     4321 agcctgggga ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc
     4381 aggactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4441 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4501 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4561 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4621 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4681 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4741 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4801 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4861 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4921 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt acgccttaag
     4981 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     5041 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     5101 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     5161 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccctc
     5221 tagactcgag ttagaccaga ttgtactcct tcaggttcaa ctggaggatg gtgtccttga
     5281 cggcagcaaa gacaaagcgg atattctcgg tgtctgtggc gcacgtgaag tgggagtaga
     5341 taattttgtc actgtctggg ttcaggtcca cgaacatctt cagaatgaat tctcgggctg
     5401 cctgggcatc tctctggggt ccatcatatt ctgggaagta gtcgactaga tgggaataca
     5461 tgattttctc ctctagaaga tctttcttgt ttaagaacag aataaccgag gagttctgga
     5521 accaggggta tgtgataatt gttctaaaga gagccttgct ttcctccatt cggttctcat
     5581 tgtctgactc cacgagaact tgatcatatt cactaagcgc tactagaaac atgatagagg
     5641 tgacattttc aaagcagtgt atccattttc ttctctctga cctttggccc cctacatcga
     5701 ccattctgaa aatgacactt tgtaagtcaa aggggtattc gatgatccct gtggtgggga
     5761 ctcgaactct aagcacatct tgttgcgtag gcaggtaggc agggtcagct acgcggtcca
     5821 agtcattaag atagtatttg gtagagtcag ataattgata ttctcgtcgt ctatcatagc
     5881 attcctggat tccaggatca ttccataaac tctttattgc atctacgtat atgccgagag
     5941 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     6001 ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     6061 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt
     6121 tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga
     6181 cgttgtcgct aaagtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     6241 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     6301 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     6361 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc
     6421 actgcacgcc ccaggacagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     6481 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     6541 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     6601 cggtgaacag ctcctcgccc ttgctcacca tgctgcctcc gcctccgctt ggatcctcaa
     6661 aagcagacac cttctccaca tcaacttctc gaactaattg tgcatgagcc ttattgtgct
     6721 catacttgta tgggatcttg agtgtgtcca tggctctgat catggcctgc atggccgtga
     6781 agatgttctg atacaccagc ttggtgaagc cccttttatc ttcatcagag tatcctgacc
     6841 catggatgat tctcatctgc ttgataaacg tactcttgcc actctctcct gtcccgagca
     6901 gcagcagctt gagctcccgg cgggcgtccc gcttgtccct gcggagctgc cgctcgatct
     6961 cgtcgttgat ccgccgggct tccttggcct cctcgctcag gcagcacgcc atgatggact
     7021 ccagagtcat ggttgtggcc atattatcat cgtgtttttc aaaggaaaac cacgtccccg
     7081 tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac
     7141 cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg
     7201 ttgaatacgc ttgaggagag ccatttgact ctttccacaa ctatccaact cacaacgtgg
     7261 cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg
     7321 cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tc
//
