LOCUS       sequence-190871-        3726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the construction of DNA-protein hybrid nanostructures. 
            Template for the three-armed star shown in figure 3D of associated 
            article..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Praetorius F, Dietz H
  TITLE     Self-assembly of genetically encoded DNA-protein hybrid nanoscale 
            shapes.
  JOURNAL   Science. 2017 Mar 24;355(6331). pii: eaam5488. doi: 
            10.1126/science.aam5488.
  PUBMED    28336611
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-03-24"; volume: "355(6331). pii: eaam5488. doi"; pages: 
            " 10.1126/science.aam5488"
COMMENT     Sequence Label: pexK4_wm_ruleB
FEATURES             Location/Qualifiers
     source          1..3726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..30)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(184..772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(264..283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1074..1868
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1128..1147)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1738..1757
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2033..2051
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2089..2111)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2211..2230
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3521..3543)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3532..3548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3556..3562,3563..3580,3581..3586))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3601..3622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
       61 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      601 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      901 taaatcaatc taaagtatat atgagtaaaa atattccgga attgccagct ggggcgccct
      961 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct
     1021 gatggcgcag gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     1081 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     1141 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     1201 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     1261 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     1321 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     1381 tgtcatccca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     1441 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     1501 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     1561 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     1621 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     1681 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     1741 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     1801 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     1861 tcttctgaac cggtaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1921 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1981 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     2041 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     2101 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     2161 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     2221 gcagattgta ctgagagtgc accaattggt cgacctcgag ttaattaacg taagatcgca
     2281 caaattatca cgaatactgt ggaaagtagc tagtatttca tccaatagca gttacacgtc
     2341 agttacgtcg agcagaattc ctatactcat tcatgttata acggacgggt atagatagtg
     2401 agggtctcta atgtcccaag gaaaaagagc aattcttcca tcctggatag agtttgggca
     2461 tgtaagattt tatcactgta aactggttgc accattataa atgtcggttg ccgtcttcag
     2521 taaattcgag atcaatcgat agtaattgtc catgccttta tgcttgcgtt agaggaccta
     2581 tcacaagtac aagtcttact gtatatccgg actaacaccg gctctgaggt tgacgccaca
     2641 gagggtaccg cagccctcct tagcatagct agccctaagt gagcttcagg ccatgagaca
     2701 gccaaggggt cagtcgcaat agtcggatga gcgtcagggg gtagtaggta ttagaggtgc
     2761 gctgtaggta tcgactgtat catcagcgga tcatctcgat cggagccagg taaatgtgga
     2821 ttcaccggag atctgagccc tgtcaatacg cgagcattct agttggctca ttgctcagtg
     2881 tactgctaaa ctcacccgat aggacttcag tagtaccgat agtatcgata gttagcctgc
     2941 attagtgagt gacaacgaga caaaaacgtt cgacgaaacg ttacgcctcc acaagaattc
     3001 gagatctatg aattatgagg tcgttgtacc gggaattcgg cgcccacgcc taagccgaca
     3061 tgggtcggcg aggcactccc ggccagcacc gccggagact ttacagcctg actttttgaa
     3121 gtccatttag gaccgcggcc acacagtggg gcgtacatgt tttcaccagg ctaccaaata
     3181 acgaaagtga cgcgcgaaca atgtggggta cgatacaggc tggaccgcgg cgcttaataa
     3241 tggcgggatg tttcaatatc gattcatctg aattgaggca ggcatcgcta acacggaccg
     3301 gagtgatata taaaattaca agtggctcat tggcgaacga ggctgagaat acccgtgggg
     3361 acaagggact gatcccaatc caagagcgct gattacatta cgtcctgtca agagttgtcg
     3421 gttcacgagc taagagttca attcagatga tcgctgtcgt tctagcctaa ttaccagtac
     3481 atgtataact agtgggcgat cgctctagag ctagcgaatt cctgtgtgaa attgttatcc
     3541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     3601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3661 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3721 tgggcg
//
