LOCUS       sequence-190875-        5225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the 
            farnesylated fluorescent protein mKate2.5 from the GFAP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Chan KY, Jang MJ, Yoo BB, Greenbaum A, Ravi N, Wu WL, 
            Sanchez-Guardado L, Lois C, Mazmanian SK, Deverman BE, Gradinaru V
  TITLE     Engineered AAVs for efficient noninvasive gene delivery to the 
            central and peripheral nervous systems.
  JOURNAL   Nat Neurosci. 2017 Aug;20(8):1172-1179. doi: 10.1038/nn.4593. Epub 
            2017 Jun 26.
  PUBMED    28671695
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.4593"; 
            journalName: "Nat Neurosci"; date: "2017-08"; volume: "20"; issue: 
            "8"; pages: "1172-1179"
COMMENT     Sequence Label: pAAV-GFAP-mKate2.5f
FEATURES             Location/Qualifiers
     source          1..5225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    836..854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    872..890
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             962..1657
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSKTFINHPQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPANGPVMQKKTLGWEASTETLYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPAKNLKMPGVYNVDRRLERIKEADNETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    1728..2316
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1781..1801)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2199..2210)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2879..3000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2890..2909)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2944..2963
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3103..3232
                     /label=AAV2 ITR
     primer_bind     complement(3297..3315)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3383..3487
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3488..3556,3557..4348)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4519..5107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5008..5027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg
       61 cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt
      121 cctactagta acatatcctg gtgtggagta ggggacgctg ctctgacaga ggctcggggg
      181 cctgagctgg ctctgtgagc tggggaggag gcagacagcc aggccttgtc tgcaagcaga
      241 cctggcagca ttgggctggc cgccccccag ggcctcctct tcatgcccag tgaatgactc
      301 accttggcac agacacaatg ttcggggtgg gcacagtgcc tgcttcccgc cgcaccccag
      361 cccccctcaa atgccttccg agaagcccat tgagcagggg gcttgcattg caccccagcc
      421 tgacagcctg gcatcttggg ataaaagcag cacagccccc taggggctgc ccttgctgtg
      481 tggcgccacc ggcggtggag aacaaggctc tattcagcct gtgcccagga aaggggatca
      541 ggggatgccc aggcatggac agtgggtggc agggggggag aggagggctg tctgcttccc
      601 agaagtccaa ggacacaaat gggtgagggg agagctctcc ccatagctgg gctgcggccc
      661 aaccccaccc cctcaggcta tgccaggggg tgttgccagg ggcacccggg catcgccagt
      721 ctagcccact ccttcataaa gccctcgtat cccaggagcg agcagagcca gagcaggttg
      781 gagaggagac gcatcacctc tgctgcttgc ctaaaacagg taagtcccat taatctccct
      841 atcagtgata gagaaggtct gaagagttta ctccctatca gtgatagaga ttaattcctc
      901 tactaacctt gttcatcttt tctttttttt tctacaggtc ctgggtgatt aacagcttaa
      961 gatggtgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     1021 gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     1081 gaccatgaga atcaaggtcg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     1141 taccagcttc atgtacggca gcaaaacctt catcaaccac cctcagggca tccccgactt
     1201 ctttaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
     1261 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     1321 caagattaga ggggtgaact tcccagccaa cggccctgtg atgcagaaga aaacactcgg
     1381 ctgggaggcc tccaccgaga cgctgtaccc cgctgacggc ggcctggaag gcgcatgtga
     1441 catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttggaga ccacatacag
     1501 atccaagaaa cccgctaaga acctcaagat gcccggcgtc tacaacgtgg acaggagact
     1561 ggaaagaatc aaggaggccg acaatgagac ctacgtcgag cagcacgagg tggctgtggc
     1621 cagatactct actggtggcg ctggtgatgg aggtaaaaag ctgaaccctc ctgatgagag
     1681 tggccccggc tgcatgagct gcaagtgtgt gctctcctga ggatcctaat caacctctgg
     1741 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     1801 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     1861 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     1921 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     1981 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     2041 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     2101 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     2161 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     2221 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     2281 agacgagtcg gatctccctt tgggccgcct ccccgcctga attctgcata cttctttaca
     2341 ttccagatat ctgcatcaaa caccattgtc acactccatc gcgatcacgc gagccgaacg
     2401 aacaaaccca acaacatgaa actacctagc gcttctattc agcatgatgt ctttcgtata
     2461 cacaaattcg gttctacagg gtaacctagg ctgcatgttt aaacatacat acttctttac
     2521 attccagata tctgcatcaa acaccattgt cacactccat cgcgatcacg cgagccgaac
     2581 gaacaaaccc aacaacatga aactacctag cgcttctatt cagcatgatg tctttcgtat
     2641 acacaaattc ggttctacag ggtaacctag gctgcatgtt taaacataca tacttcttta
     2701 cattccagat atctgcatca aacaccattg tcacactcca tcgcgatcac gcgagccgaa
     2761 cgaacaaacc caacaacatg aaactaccta gcgcttctat tcagcatgat gtctttcgta
     2821 tacacaaatt cggttctaca gggtaaccta ggctgcaggt accttcgagc agacatgata
     2881 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2941 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3001 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
     3061 taaagcaagt aaaacctcta caaatgtggt aaaatcaagc ttaggaaccc ctagtgatgg
     3121 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     3181 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agagagggag
     3241 tggccaagct agcgggcgat taaggaaagg gctagatcat tcttgaagac gaaagggcct
     3301 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     3361 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3421 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3481 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     3541 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     3601 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     3661 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     3721 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     3781 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     3841 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3901 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3961 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4021 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4081 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4141 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4201 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4261 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4321 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     4381 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4441 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4501 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4561 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4621 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4681 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4741 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4801 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4861 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4921 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4981 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5041 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5101 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5161 tcacatgtaa taaacacaca cacaccaaca accgtggttg gttgttgtgt tggtttattc
     5221 tcgag
//
