LOCUS       sequence-19088-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf3
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             542..583
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             593..610
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(633..650)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    639..750
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcaccgaatt ccagctgacc accatggtcg atgaaatatc tgatgttgtt gttcgctgct
       61 atgataatca gatcattcgc cgatagtggt aacgctatcg aaacgacatc gccagaaatt
      121 acaaacgcca caacagatat tccagctatc agattatgcg gtccagaggg agatggatat
      181 tgtttacacg gtgactgtat ccacgctaga aatattgacg gtatgtattg tagatgctct
      241 catggttata caggcattag atgtcagcat gtagtattag tagactatca acgttcagaa
      301 aacccaaaca ctacaacgtc atatatccca tctcccggta ttatgcttgt attagtaggc
      361 attattatta ttacgtgttg tctattatct gtttataggt tcactcgacg aactaaacta
      421 cctatacaag atatggttgt gccataagca attcccgggg atccgaataa agggtcaaga
      481 caattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
      541 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
      601 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
      661 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
      721 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agt
//
