LOCUS       sequence-190881-        5253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An AAV genome that expresses the fluorescent protein mRuby2 from the
            hSyn1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Chan KY, Jang MJ, Yoo BB, Greenbaum A, Ravi N, Wu WL, 
            Sanchez-Guardado L, Lois C, Mazmanian SK, Deverman BE, Gradinaru V
  TITLE     Engineered AAVs for efficient noninvasive gene delivery to the 
            central and peripheral nervous systems.
  JOURNAL   Nat Neurosci. 2017 Aug;20(8):1172-1179. doi: 10.1038/nn.4593. Epub 
            2017 Jun 26.
  PUBMED    28671695
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.4593"; 
            journalName: "Nat Neurosci"; date: "2017-08"; volume: "20"; issue: 
            "8"; pages: "1172-1179"
COMMENT     Sequence Label: pAAV-hSyn1-mRuby2
FEATURES             Location/Qualifiers
     source          1..5253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      636..645
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             642..1352
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     misc_feature    1380..1968
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1433..1453)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1851..1862)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2000..2476
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2199..2218)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2516..2656
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2516..2645
                     /label=AAV2 ITR
     rep_origin      2731..3186
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2818..2837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3028..3049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3203..3222)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3322..3344
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3382..3400)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3468..3572
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3573..3641,3642..4433)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3791..3810)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4604..5192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5093..5112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccgccac catggtgagc aagggagagg
      661 aactcatcaa ggagaacatg cgcatgaagg tagtcatgga agggtctgtg aatgggcatc
      721 agttcaagtg cacaggggaa ggtgaaggga atccctacat gggtactcag accatgagga
      781 tcaaggttat tgaaggtggc cctctcccgt ttgccttcga cattcttgcc actagcttca
      841 tgtatggcag tcggaccttc atcaagtacc ccaaaggcat tccagacttc ttcaaacagt
      901 cctttccaga gggcttcaca tgggaaaggg tgacccgtta cgaggatggt ggtgtggtca
      961 cagtcatgca ggacactagt ctggaggacg gatgtctggt ctatcacgtg caagtgagag
     1021 gggtgaactt tccctccaat gggcctgtga tgcagaagaa aaccaaaggg tgggagccaa
     1081 acacagagat gatgtatcct gcagatggag gactgagagg ctatacgcac atggctctga
     1141 aggttgacgg aggaggccac ttgtcatgct catttgtgac cacgtacagg agcaagaaaa
     1201 ctgtcggcaa catcaaaatg cctggcatac atgcggttga tcaccgcttg gaacgactgg
     1261 aggagtctga caacgagatg tttgtggtac aacgggaaca tgcagttgcc aagtttgctg
     1321 gccttggcgg aggaatggat gagctgtaca aatgagaatt cgatatcaag cttatcgata
     1381 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     1441 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     1501 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     1561 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     1621 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     1681 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     1741 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     1801 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     1861 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     1921 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag
     1981 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     2041 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     2101 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     2161 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     2221 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     2281 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     2341 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     2401 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     2461 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa
     2521 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     2581 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     2641 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2701 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     2761 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     2821 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2881 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2941 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3001 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3061 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     3121 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     3181 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     3241 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     3301 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3361 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3421 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3481 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3541 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3601 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3661 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3721 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3781 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3841 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3901 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3961 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4021 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4081 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4141 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4201 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4261 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4321 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4381 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4441 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4501 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4561 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4621 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4681 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4741 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4801 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4861 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4921 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4981 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5041 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5101 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5161 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5221 tcctggcctt ttgctggcct tttgctcaca tgt
//
