LOCUS       sequence-19089-e         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf4
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             284..325
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             335..352
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(375..392)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    381..492
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cattctacat tttttaatcg tctatatagt atttttctga tattttctat aatatcagaa
       61 ttgtcttcca tcggaagttg tatactatcg gaatcagtta catgtttaaa taattctctg
      121 atgtcattcc ttatacaatc aaattcatta ttaaacagtt taatagtctg tagaccttta
      181 tcgtcgtaag caattcccgg ggatccgaat aaagggtcaa gacaattctg cagatatcca
      241 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
      301 ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta
      361 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
      421 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
      481 ggaaattgca tcgcattgtc tgagtaggtg tcattc
//
