LOCUS       sequence-19090-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf5
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             675..716
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             726..743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accgaatttc cagctgacca ccatgggaat ttcgattcct gccaaaatca atacatcatc
       61 tatagaccat gtaacaatat tacaatacat agatgaacca aatgatataa gactaacagt
      121 atgcattatc cgaaatatta ataacattac atattatatc aatatcacaa aaataaatac
      181 acatttggct aatcaatttc gggcttggaa aaaacgtatc gccggaaggg actatatgac
      241 taacttatct agagatacag gaatacaaca atcaaaactt actgaaacta tacgtaactg
      301 tcaaaaaaat agaaacatat atggtctata tatacactac aatttagtta ttaatgtggt
      361 tattgattgg ataaccgatg tgattgttca atcaatatta agagggttgg taaattggta
      421 catagctaat aatacctata ctccaaatac acccaataat acaacaacca tttctgagtt
      481 ggatatcatc aaaatactgg ataaatacga ggacgtgtat agagtaagta aagaaaaaga
      541 atgtggaatt tgctatgaag ttgtttactc aaaacgatag gcaattcccg gggatccgaa
      601 taaagggtca agacaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
      661 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
      721 ccggtcatca tcaccatcac cattga
//
