LOCUS       sequence-190916-        7147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express FtsZ tagged with pAmCherry1 in E coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Buss J, Coltharp C, Shtengel G, Yang X, Hess H, Xiao J
  TITLE     A multi-layered protein network stabilizes the Escherichia coli 
            FtsZ-ring and modulates constriction dynamics.
  JOURNAL   PLoS Genet. 2015 Apr 7;11(4):e1005128. doi: 
            10.1371/journal.pgen.1005128. eCollection 2015 Apr.
  PUBMED    25848771
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2015-04-7"; volume: "11(4):e1005128. doi"; pages: " 
            10.1371/journal.pgen.1005128. eCollection 2015 Apr"
COMMENT     Sequence Label: pJB066
FEATURES             Location/Qualifiers
     source          1..7147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             312..334
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1510..2220
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein"
                     /label=PAmCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1655..1673)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1923..1942
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(2492..3283)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(4535..5485)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5533..5755
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(6243..7121)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcaaaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagaaataa ttttgtttaa ctttaagaag gagatatacg ctagcatgtt tgaaccaatg
      361 gaacttacca atgacgcggt gattaaagtc atcggcgtcg gcggcggcgg cggtaatgct
      421 gttgaacaca tggtgcgcga gcgcattgaa ggtgttgaat tcttcgcggt aaataccgat
      481 gcacaagcgc tgcgtaaaac agcggttgga cagacgattc aaatcggtag cggtatcacc
      541 aaaggactgg gcgctggcgc taatccagaa gttggccgca atgcggctga tgaggatcgc
      601 gatgcattgc gtgcggcgct ggaaggtgca gacatggtct ttattgctgc gggtatgggt
      661 ggtggtaccg gtacaggtgc agcaccagtc gtcgctgaag tggcaaaaga tttgggtatc
      721 ctgaccgttg ctgtcgtcac taagcctttc aactttgaag gcaagaagcg tatggcattc
      781 gcggagcagg ggatcactga actgtccaag catgtggact ctctgatcac tatcccgaac
      841 gacaaactgc tgaaagttct gggccgcggt atctccctgc tggatgcgtt tggcgcagcg
      901 aacgatgtac tgaaaggcgc tgtgcaaggt atcgctgaac tgattactcg tccgggtttg
      961 atgaacgtgg actttgcaga cgtacgcacc gtaatgtctg agatgggcta cgcaatgatg
     1021 ggttctggcg tggcgagcgg tgaagaccgt gcggaagaag ctgctgaaat ggctatctct
     1081 tctccgctgc tggaagatat cgacctgtct ggcgcgcgcg gcgtgctggt taacatcacg
     1141 gcgggcttcg atctgcgtct ggatgagttc gaaacggtag gtaacaccat ccgtgcattt
     1201 gcttccgaca acgcgactgt ggttatcggt acttctcttg acccggatat gaatgacgag
     1261 ctgcgcgtaa ccgttgttgc gacaggtatc ggcatggaca aacgtcctga aatcactctg
     1321 gtgaccaata agcaggttca gcagccagtg atggatcgct accagcagca tgggatggct
     1381 ccgctgaccc aggagcagaa gccggttgct aaagtcgtga atgacaatgc gccgcaaact
     1441 gcgaaagagc cggattatct ggatatccca gcattcctgc gtaagcaagc tgatggccta
     1501 tgcggccgca tggtgagcaa gggcgaggag gataacatgg ccatcattaa ggagttcatg
     1561 cgcttcaagg tgcacatgga ggggtccgtg aacggccacg tgttcgagat cgagggcgag
     1621 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     1681 cccctgccct tcacctggga catcctgtcc cctcaattca tgtacggctc caatgcctac
     1741 gtgaagcacc ccgccgacat ccccgactac tttaagctgt ccttccccga gggcttcaag
     1801 tgggagcgcg tgatgaaatt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     1861 ctgcaggacg gtgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     1921 ggccccgtaa tgcagaagaa gaccatgggc tgggaggccc tctccgagcg gatgtacccc
     1981 gaggacggcg ccctgaaggg cgaggtcaag ccgagagtga agctgaagga cggcggccac
     2041 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     2101 tacaacgtca accgcaagtt ggacatcacc tcacacaacg aggactacac catcgtggaa
     2161 cagtacgaac gtgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     2221 gtcgacctgc aggcatgcaa gcttagtaaa gccctcgcta gattttaatg cggatgttgc
     2281 gattacttcg ccaactattg cgataacaag aaaaagccag cctttcatga tatatctccc
     2341 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag
     2401 tttggctgtg agcaattatg tgcttagtgc atctaacgct tgagttaagc cgcgccgcga
     2461 agcggcgtcg gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc
     2521 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     2581 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     2641 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     2701 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     2761 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     2821 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     2881 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     2941 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
     3001 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
     3061 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     3121 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
     3181 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
     3241 ctgatagttg agtcgatact tcggcgatca ccgcttccct catgatgttt aactttgttt
     3301 tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca
     3361 cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt
     3421 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     3481 tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt
     3541 atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg
     3601 gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct
     3661 ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg
     3721 atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc
     3781 tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg
     3841 cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg cgggtcaagg
     3901 atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc aaggatcggg
     3961 ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcaggggaat taattcccac
     4021 gggttttgct gcccgcaaac gggctgttct ggtgttgcta gtttgttatc agaatcgcag
     4081 atccggcttc agccggtttg ccggctgaaa gcgctatttc ttccagaatt gccatgattt
     4141 tttccccacg ggaggcgtca ctggctcccg tgttgtcggc agctttgatt cgataagcag
     4201 catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg
     4261 ttcaatttca tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac
     4321 atgctgttca tctgttacat tgtcgatctg ttcatggtga acagctttga atgcaccaaa
     4381 aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga
     4441 cagttttccc tttgatatgt aacggtgaac agttgttcta cttttgtttg ttagtcttga
     4501 tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta
     4561 tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat
     4621 acttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag
     4681 ttaaagcatc gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct gatgtaatgg
     4741 ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat
     4801 ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat
     4861 caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc
     4921 attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt
     4981 catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata
     5041 accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat
     5101 attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt
     5161 ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa
     5221 ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata
     5281 caccataagc attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg
     5341 ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc
     5401 ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg
     5461 ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt taatcactat
     5521 accaattgag atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta
     5581 tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa
     5641 attccgctag acctttgtgt gttttttttg tttatattca agtggttata atttatagaa
     5701 taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac
     5761 tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa
     5821 acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga
     5881 atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag
     5941 ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta
     6001 tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg
     6061 cgttttatgg cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg
     6121 ccctctgatt ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc
     6181 taatgcaccc agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtcgggaat
     6241 tcttatgaca acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg
     6301 ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa
     6361 ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc
     6421 tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa
     6481 agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa
     6541 ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc
     6601 ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga
     6661 tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca
     6721 aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca
     6781 aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac
     6841 tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg
     6901 aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc
     6961 tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa
     7021 aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac
     7081 gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca
     7141 ttcagag
//
