LOCUS       sequence-19094-e         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf9
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             357..398
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             408..425
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(448..465)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    454..565
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggatccagta cccttcaccg aattccagct gaccaccatg actttagtaa agtcgtctat
       61 attaactctt ttattttcta gccatcgtaa taccatgttt aagatagtat attctctagt
      121 tactacgatc tcatcgttgt ctagaatatc acatactgaa tctacatcca attttagaaa
      181 ttggtctgtg ttacatatct cttctatatt attgttgatg tattgtcgta gaaaactatt
      241 acgtagacca ttttctttat aagcaattcc cggggatccg aataaagggt caagacaatt
      301 ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta
      361 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc
      421 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
      481 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
      541 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctgggg
//
